Detection and Species Identification of<i>Leishmania</i>DNA from Filter Paper Lesion Impressions for Patients with American Cutaneous Leishmaniasis

Boggild, Andrea K.; Valencia, Braulio M.; Espinosa, Diego A.; Veland, Nicolás; Ramos, Ana Pilar; Arévalo, Jorge; Llanos-Cuentas, Alejandro; Low, Donald E.

doi:10.1086/648730

Cited by 63 publications

(115 citation statements)

References 21 publications

Supporting

Mentioning

110

Contrasting

Unclassified

Order By: Relevance

“…Data were collected as a sub-study of a comparative diagnostic evaluation at the Leishmania Clinic of the Instituto de Medicina Tropical "Alexander Von Humboldt," Hospital Nacional Cayetano Heredia, in Lima, Peru, between January and April of 2009 and 2010, the results of which are reported elsewhere. 15 The study was approved by the Institutional Review Board of the Universidad Peruana Cayetano Heredia, Lima, Peru, and the University of Toronto, before initiation. Consecutive patients presenting to the Leishmania Clinic for the evaluation of skin lesions between January and April 2009 and 2010 were enrolled as described elsewhere.…”

Section: Methodsmentioning

confidence: 99%

“…For the comparative diagnostic evaluation, skin scraping, aspirate specimens, and filter paper lesion impressions were obtained for Giemsa-stained smear, culture, and PCR as described elsewhere. [15][16][17] kDNA PCR of lesion scrapings and aspirates was performed as described using primers specific for Leishmania ( Viannia ) kDNA. [15][16][17][18][19] A single, mid-stream urine specimen was collected from each participant on enrollment before the diagnostic evaluations.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Polymerase Chain Reaction Detection of Leishmania kDNA from the Urine of Peruvian Patients with Cutaneous and Mucocutaneous Leishmaniasis

Veland¹,

Espinosa²,

Valencia³

et al. 2011

The American Society of Tropical Medicine and Hygiene

Self Cite

View full text Add to dashboard Cite

Abstract. We hypothesized that Leishmania kDNA may be present in urine of patients with cutaneous leishmaniasis (CL). Urine samples and standard diagnostic specimens were collected from patients with skin lesions. kDNA polymerase chain reaction (PCR) was performed on samples from patients and 10 healthy volunteers from non-endemic areas. Eighty-six of 108 patients were diagnosed with CL and 18 (21%) had detectable Leishmania Viannia kDNA in the urine. Sensitivity and specificity were 20.9% (95% confidence interval [CI] 12.3-29.5%) and 100%. Six of 8 patients with mucocutaneous involvement had detectable kDNA in urine versus 12 of 78 patients with isolated cutaneous diseaseNo healthy volunteer or patient with an alternate diagnosis had detectable kDNA in urine. Sensitivity of urine PCR is sub-optimal for diagnosis. On the basis of these preliminary data in a small number of patients, detectable kDNA in urine may identify less localized forms of infection and inform treatment decisions.

show abstract

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Polymerase Chain Reaction Detection of Leishmania kDNA from the Urine of Peruvian Patients with Cutaneous and Mucocutaneous Leishmaniasis

Veland¹,

Espinosa²,

Valencia³

et al. 2011

The American Society of Tropical Medicine and Hygiene

Self Cite

View full text Add to dashboard Cite

show abstract

“…Material from lesion scrapings was smeared on glass slides for Giemsa-stained microscopy and assayed for detection of Leishmania (Viannia) kinetoplast DNA (kDNA) by polymerase chain reaction (PCR) as described ( Figure 1A). 15 Tissue fluid from lesion aspirates was inoculated into traditional Leishmania NovyMacNeal-Nicolle culture medium as described, 16 and assayed by kDNA PCR. Filter paper lesion impressions (FPLIs) were assayed by kDNA PCR as described.…”

Section: Case Reportmentioning

confidence: 99%

“…Filter paper lesion impressions (FPLIs) were assayed by kDNA PCR as described. 15,16 Identification of Leishmania (Viannia) causative species was performed by using mannose phosphate isomerase (mpi), cysteine proteinase B, and heat shock protein 70 PCR and PCR-restriction fragment length polymorphism (PCR-RFLP) analysis according to the algorithm described elsewhere. 17 Specimens from lesions 1 and 2 on the arms amplified product in the mpi PCR when mpi Leishmania (V.) braziliensis primer Figure 1B).…”

Section: Case Reportmentioning

confidence: 99%

Simultaneous Infection with Leishmania (Viannia) braziliensis and L. (V.) lainsoni in a Peruvian Patient with Cutaneous Leishmaniasis

Veland

Valencia²,

Alba³

et al. 2013

The American Society of Tropical Medicine and Hygiene

Self Cite

View full text Add to dashboard Cite

show abstract

“…5 Cutaneous leishmaniasis (CL) is frequently diagnosed by classic clinical investigation, or via laboratorial diagnosis under microscopic examination of smears or biopsies of skin lesions. 9,10 Culturing of replicative Leishmania specimens from the skin lesion border may also be performed. 4,10,11 These methods, however, often lead to misdiagnosis; the culture assays are positive in only ca.…”

mentioning

confidence: 99%

Biosensors for Efficient Diagnosis of Leishmaniasis: Innovations in Bioanalytics for a Neglected Disease

et al. 2010

View full text Add to dashboard Cite

The need for reliable, fast diagnostics is closely linked to the need for safe, effective treatment of the so-called “neglected” diseases. The list of diseases with no field-adapted diagnostic tools includes leishmaniasis, shigella, typhoid, and bacterial meningitis. Leishmaniasis, in particular, is a parasitic disease caused by Leishmania spp. transmitted by infected phlebotomine sandfly, which remains a public health concern in developing countries with ca. 12 million people infected and 350 million at risk of infection. Despite several attempts, methods for diagnosis are still noneffective, especially with regard to specificity due to false positives with Chagas’ disease caused by Trypanosoma cruzi. Accepted golden standards for detecting leishmaniasis involve isolation of parasites either microscopically, or by culture, and in both methods specimens are obtained by invasive means. Here, we show that efficient distinction between cutaneous leishmaniasis and Chagas’ disease can be obtained with a low-cost biosensor system made with nanostructured films containing specific Leishmania amazonensis and T. cruzi antigens and employing impedance spectroscopy as the detection method. This unprecedented selectivity was afforded by antigen−antibody molecular recognition processes inherent in the detection with the immobilized antigens, and by statistically correlating the electrical impedance data, which allowed distinction between real samples that tested positive for Chagas’ disease and leishmaniasis. Distinction could be made of blood serum samples containing 10−5 mg/mL of the antibody solution in a few minutes. The methods used here are generic and can be extended to any type of biosensor, which is important for an effective diagnosis of many other diseases.

show abstract

Detection and Species Identification ofLeishmaniaDNA from Filter Paper Lesion Impressions for Patients with American Cutaneous Leishmaniasis

Cited by 63 publications

References 21 publications

Polymerase Chain Reaction Detection of Leishmania kDNA from the Urine of Peruvian Patients with Cutaneous and Mucocutaneous Leishmaniasis

Polymerase Chain Reaction Detection of Leishmania kDNA from the Urine of Peruvian Patients with Cutaneous and Mucocutaneous Leishmaniasis

Simultaneous Infection with Leishmania (Viannia) braziliensis and L. (V.) lainsoni in a Peruvian Patient with Cutaneous Leishmaniasis

Biosensors for Efficient Diagnosis of Leishmaniasis: Innovations in Bioanalytics for a Neglected Disease

Contact Info

Product

Resources

About