Detection and serotype distribution of <i>Actinobacillus actinomycetemcomitans</i> in cardiovascular specimens from Japanese patients

Nakano, Kazuhiko; Inaba, Hiroaki; Nomura, Ryota; Nemoto, Hirotoshi; Tamura, Kiyoko; Miyamoto, Eriko; Yoshioka, Hideo; Taniguchi, Koichi; Amano, Atsuo; Ooshima, Takashi

doi:10.1111/j.1399-302x.2007.00332.x

Cited by 41 publications

(37 citation statements)

References 26 publications

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“…Viable A. actinomycetemcomitans has also been isolated from atherosclerotic lesions, suggesting the periodontal pathogen is associated with the pathogenesis of this leading cause of death in the United States (20). Notably, a recent report indicated that A. actinomycetemcomitans was detected in 31% of cardiovascular specimens of endocarditis or aneurysm, using a molecular approach (24). This finding suggests that A. actinomycetemcomitans contributes to cardiovascular diseases more than was previously suggested by the blood culture data (4).…”

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confidence: 38%

“…on May 11, 2018 by guest http://iai.asm.org/ vascular diseases, including infective endocarditis (4,10,26), atherosclerosis (20), and aneurysm (24). These findings are not surprising, since periodontal pathogens can enter the bloodstream through daily activities, including tooth brushing and chewing food, especially in individuals with poor oral hygiene (12,37).…”

Section: Vol 76 2008mentioning

confidence: 93%

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EmaA, a Potential Virulence Determinant of Aggregatibacter actinomycetemcomitans in Infective Endocarditis

Tang¹,

Kitten

Munro

et al. 2008

Infect Immun

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The gram-negative fastidious human oropharyngeal Aggregatibacter actinomycetemcomitans is implicated in the etiology of infective endocarditis. EmaA, an oligomeric coiled-coil adhesin homologous to YadA of Yersinia enterocolitica, was hypothesized to mediate the interaction of A. actinomycetemcomitans with collagen. Collagen, the most abundant protein in human bodies and the main component of extracellular matrix (ECM), predominates in the supporting tissue of cardiac valves. To extend our earlier studies using purified collagen to determine bacterial binding activities, we developed a tissue model using rabbit cardiac valves to investigate the interaction of A. actinomycetemcomitans with native collagen. The resected mitral valves, with or without removal of the endothelium, were incubated with equivalent numbers of the wild type and the isogenic emaA mutant defective in collagen binding. There was no difference in binding between the wild-type and the mutant strains when the endothelium remained intact. However, the emaA mutant was fivefold less effective than the wild-type strain in colonizing the exposed ECM. A 10-fold increase in the binding of the wild-type strain to ECM was observed compared with the intact endothelium. Similar observations were replicated in an in vivo endocarditis rabbit model; the emaA mutant was 10-fold less effective in the initial infection of the traumatized aortic valve. Colocalization studies indicated that A. actinomycetemcomitans bound to type I collagen. A. actinomycetemcomitans preferentially colonized the ECM and, together with the evidence that EmaA interacts with the native collagen, suggested that the adhesin is likely a potential virulence determinant of the bacterium in the initiation of infective endocarditis.

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confidence: 38%

Section: Vol 76 2008mentioning

confidence: 93%

EmaA, a Potential Virulence Determinant of Aggregatibacter actinomycetemcomitans in Infective Endocarditis

Tang¹,

Kitten

Munro

et al. 2008

Infect Immun

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“…Esto puede aumentar la gravedad de la enfermedad y mantener el estado proinflamatorio. Sin embargo, la mayoría de estudios sobre la presencia de microorganismos periodontales patógenos en muestras cardiovasculares, se ha realizado en ateromas (9,11,(31)(32)(33),y en los estudios relacionados con válvulas cardiacas, se han reportado principalmente patógenos como Streptococcus mutans (34).…”

Section: Discussionunclassified

Periodontal microbiota and microorganisms isolated from heart valves in patients undergoing valve replacement surgery in a clinic in Cali, Colombia

Moreno¹,

Parra²,

Botero³

et al. 2017

biomedica

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Introducción. La periodontitis es una enfermedad infecciosa que afecta los tejidos de soporte del diente y se asocia con diferentes enfermedades sistémicas, incluida la enfermedad cardiovascular. Los estudios microbiológicos permiten detectar microorganismos a partir de muestras subgingivales y cardiovasculares.Objetivo. Describir la microbiota periodontal cultivable y la presencia de microorganismos en válvulas cardiacas de pacientes sometidos a cirugía de reemplazo valvular en una clínica de Cali.Materiales y métodos. Se analizaron 30 muestras subgingivales y de tejidos valvulares mediante cultivo en medio bifásico, agar de sangre con suplemento y agar tripticasa de soya con antibiótico. Las muestras de las válvulas se analizaron mediante reacción en cadena de la polimerasa (PCR) convencional.Resultados. Los patógenos periodontales aislados de bolsas periodontales fueron Fusobacterium (50 %), Prevotella intermedia/nigrescens (40 %), Campilobacter rectus (40 %), Eikenella corrodens (36,7 %), bacilos entéricos Gram negativos (36,7 %), Porphyromonas gingivalis (33,3 %) y Eubacterium (33,3 %). Los agentes patógenos aislados de la válvula aórtica fueron Propionibacterium acnes (12 %), bacilos entéricos Gram negativos (8 %), Bacteroides merdae (4 %) y Clostridium bifermentans (4 %), y de la válvula mitral, P. acnes y Clostridium beijerinckii. La PCR convencional no arrojó resultados positivos para agentes patógenos orales y solo se detectó ADN bacteriano en dos muestras.Conclusiones. La microbiota periodontal de pacientes sometidos a cirugía de reemplazo valvular estaba conformada por especies Gram negativas que han sido relacionadas con infecciones en tejidos extraorales; sin embargo, no se encontraron agentes patógenos periodontales en los tejidos de las válvulas. Aunque hubo muestras de estos tejidos y subgingivales, positivas para bacilos entéricos Gram negativos, no es posible asegurar que tuvieran el mismo origen filogenético.

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“…The frequency and severity of bacteremia correlates with the state of the periodontal tissues. Numerous studies confirm the significance of periopathogens in atherosclerotic plaque [4]. Chronic inflammation plays an important role in the mechanisms of the development of atherosclerosis [2,5,6].…”

mentioning

confidence: 91%

Prospective Analysis of the Relationship Between the State of Periodontal Tissues and Changes in Selected Cardiovascular Parameters in Patients with Type 2 Diabetes

2016

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Detection and serotype distribution of Actinobacillus actinomycetemcomitans in cardiovascular specimens from Japanese patients

Cited by 41 publications

References 26 publications

EmaA, a Potential Virulence Determinant of Aggregatibacter actinomycetemcomitans in Infective Endocarditis

EmaA, a Potential Virulence Determinant of Aggregatibacter actinomycetemcomitans in Infective Endocarditis

Periodontal microbiota and microorganisms isolated from heart valves in patients undergoing valve replacement surgery in a clinic in Cali, Colombia

Prospective Analysis of the Relationship Between the State of Periodontal Tissues and Changes in Selected Cardiovascular Parameters in Patients with Type 2 Diabetes

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