Detection and quantitation of Epstein-Barr virus (EBV) DNA in EDTA whole blood samples using automated sample preparation and real time PCR

Abstract

The QIAsymphony sample preparation and artus EBV RG PCR test system is suitable for the detection and quantitation of EBV DNA in EDTA whole blood in the routine diagnostic laboratory.

Comparison of QIAsymphony Automated and QIAamp Manual DNA Extraction Systems for Measuring Epstein-Barr Virus DNA Load in Whole Blood Using Real-Time PCR

“…In our testing, both extraction systems provided lower limits of detection of approximately 300 to 600 copies/mL, consistent with results from a previous study using QIAsymphony and a commercial real-time EBV PCR assay. 8 Although the reproducibility using QIAsymphony is somewhat higher than with QIAamp in samples containing relatively high viral loads (eg, Ն10,000 copies/ mL), the differences are not dramatic (CV ϭ 6.8% to 10.0% with QIAsymphony and CV ϭ 15.2% to 18.6% with QIAamp). The two systems also agree strongly in detecting EBV DNA in clinical whole blood samples (97.1% agreement in the present study), with differences occurring only in samples having EBV DNA loads near the detection limits.…”

“…8,14 Differences in viral loads determined with the two extraction systems (versus no differences) were analyzed by the one-sample t-test. Bivariate correlations were assessed with Spearman's r statistic and a two-tailed test of significance.…”

“…In another study, viral loads measured in whole blood using the QIAsymphony extraction system and the commercial Artus EBV PCR assay were more than 10-fold higher than values determined with the easy-Mag extraction system and the commercial EBV R-gene assay. 8 Because many variables can affect EBV DNA load determinations, and because interlaboratory agreement is poor, 17,18 each laboratory should evaluate the comparability of its primary and backup extraction systems.…”

Multicenter comparative study of Epstein–Barr virus DNA quantification for virological monitoring in transplanted patients

“…15 Presently, the most widely used method for EBV DNA quantification is real-time PCR. [16][17][18][19] However, due to the lack of an international EBV DNA standard, and variability in laboratory methods (including DNA extraction and amplification) standardization remains a key issue to be addressed. Comparison of results obtained in different laboratories is, in turn, a pre-requisite to define cut-off values with clinical relevance.…”

Comparative evaluation of the QIAGEN QIAsymphony® SP system and bioMérieux NucliSens easyMAG automated extraction platforms in a clinical virology laboratory

“…[5][6][7][8][9] The QIAsymphony ® SP (QIAGEN) is a recently developed fully automated nucleic extraction system and to date there is only limited evaluation data available. [10][11][12] …”