Detection and quantification ofFusarium culmorumandFusarium graminearumin cereals using PCR assays

Nicholson, P.; Simpson, D. R.; Weston, G.; Rezanoor, H. N.; Lees, A. K.; Parry, D. W.; Joyce, D. C.

doi:10.1006/pmpp.1998.0170

Cited by 475 publications

(267 citation statements)

References 23 publications

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“…Competitive PCR and real-time PCR (qPCR) are the two methods currently applied as quantitative molecular diagnostics. In the case of F. graminearum, several TaqMan probes have been developed and used to monitor pathogen dynamics in planta, grains or stubble (Nicholson et al 1998;Waalwijk et al 2003Waalwijk et al , 2004Reischer et al 2004). …”

Section: Introductionmentioning

confidence: 99%

Correlation betweenFusarium graminearumand deoxynivalenol during the 2012/13 wheat Fusarium head blight outbreak in Argentina

Palazzini¹,

Fumero²,

Yerkovich³

et al. 2015

Cereal Research Communications

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Fusarium graminearum (Schwabe) is reported as the main causal agent of Fusarium head blight in Argentina. The disease causes great losses in humid and semi-humid regions of the world, reducing grain yield and quality. During 2012/13 harvest season, a severe epidemic occurred in Argentina. The aims of this work were to determine the F. graminearum incidence and deoxynivalenol accumulation in wheat grain and flour samples obtained from two of the main wheat growing regions from Argentina. Levels of the pathogen and deoxynivalenol content were correlated in heads, grains and flour. Out of 69 wheat grain samples, 55 (79.7%) showed deoxynivalenol levels between 0.4 and 8.5 µg/kg. Fusarium graminearum was the main species isolated, the isolation frequency ranged from 30 to 52% of the total grains analyzed. Correlations were observed between deoxynivalenol content, % of F. grami nearum infection, presence of the pathogen in heads, grain and flour.

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Section: Introductionmentioning

confidence: 99%

Correlation betweenFusarium graminearumand deoxynivalenol during the 2012/13 wheat Fusarium head blight outbreak in Argentina

Palazzini¹,

Fumero²,

Yerkovich³

et al. 2015

Cereal Research Communications

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“…Column (5) shows levels of DMSP-dependent DMS production, in nmol DMS h À1 mg À1 A. sydowii mycelial dry weight, with standard errors from two samples. Fungi were grown on solid PDA (Nicholson et al, 1998) at 28 1C for 48 h. A plug of B25 mm 2 from the growing edge of each mycelium was removed and placed in a sealed vial containing 5 mM DMSP in Vogel's minimal media. Levels of DMS were assayed after 6 h by gas chromatography in a flame photometric detector as in Todd et al (2009).…”

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confidence: 99%

The opportunistic coral pathogen Aspergillus sydowii contains dddP and makes dimethyl sulfide from dimethylsulfoniopropionate

et al. 2009

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The ascomycete Aspergillus sydowii is associated with a serious epizootic of sea fan corals in the Caribbean. Corals are rich in the compatible solute, dimethylsulfoniopropionate (DMSP), produced by their symbionts, the dinoflagellate Symbiodinium. As other Aspergillus species can catabolize DMSP, liberating dimethyl sulfide (DMS) in the process, we tested A. sydowii strains, obtained from diseased corals and other environments, for this Ddd þ phenotype. All the strains, irrespective of their geographical or environmental origins, made DMS from DMSP, and all of them contained homologs (487% identical) of the dddP gene, which encodes an enzyme that releases DMS from DMSP and which occurs in other Ddd þ fungi and in some marine bacteria. The dddP gene was likely acquired by the Aspergillus fungi by inter-domain horizontal gene transfer from a-proteobacteria.

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“…The reaction mixtures of a total volume of 25 μl contained 10×PCR buffer, 1.5 mM of MgCl 2 , 0.3 μM of each primer, 0.2 mM of dNTPs, 1 U of Taq DNA Polymerase (Thermo Scientific -Fermentas, Canada) and approximately 25 ng of fungal template DNA. PCR amplification was carried out in the Veriti 96-Well Thermal Cycler (Applied Biosystems, Carlsbad, CA, USA) using temperature profiles described by Demeke et al (2005), Koncz et al (2008), Mishra et al (2003), Mulè et al (2004a) Nicholson et al (1998), and Rahjoo et al (2008). The PCR products were visualized by 1×TBE electrophoresis in ethidiumbromide-stained 1% agarose gel.…”

Section: Methodsmentioning

confidence: 99%

Morphological and molecular identification and PCR amplification to determine the toxigenic potential of Fusarium spp. isolated from maize ears in southern Poland

Lenart

Klimek-Kopyra²,

Boroń

2013

Phytoparasitica

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The average amount of precipitation in spring and summer 2010 and 2011 coupled with relatively high temperatures caused massive Fusarium spp. infection of maize and yield losses in southern Poland. In order to examine the cause of this disease outbreak, Fusarium spp. were isolated and fungal strains were identified based on morphological characters and species-specific PCR assays. A total of 200 maize samples were processed, resulting in the obtention of 71 strains, which belonged to five Fusarium species, F. poae being the predominant one (74.56%). Other isolates were identified as F. graminearum, F. oxysporum, F. verticillioides and F. proliferatum. PCR-based detection of mycotoxinsynthesis-pathway genes was also used to determine the potential of the analyzed strains to produce trichothecenes (DON and NIV) and fumonisins (FUM).Only 14 isolates revealed the potential to produce DON (11 strains) and FUM (3 strains). HPLC analyses of grain samples revealed the presence of DON onlyother mycotoxins were not detected. Moreover, 57.1% of potentially mycotoxin-producing isolates indicated the toxicity in a biological test.

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Detection and quantification ofFusarium culmorumandFusarium graminearumin cereals using PCR assays

Cited by 475 publications

References 23 publications

Correlation betweenFusarium graminearumand deoxynivalenol during the 2012/13 wheat Fusarium head blight outbreak in Argentina

Correlation betweenFusarium graminearumand deoxynivalenol during the 2012/13 wheat Fusarium head blight outbreak in Argentina

The opportunistic coral pathogen Aspergillus sydowii contains dddP and makes dimethyl sulfide from dimethylsulfoniopropionate

Morphological and molecular identification and PCR amplification to determine the toxigenic potential of Fusarium spp. isolated from maize ears in southern Poland

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