Detection and quantification of vitamins in microliter volumes of biological samples by LC‐MS for clinical screening

Plants are regularly colonised by fungi and bacteria, but plant‐inhabiting microbes are rarely considered in studies on plant–herbivore interactions. Here we show that young gypsy moth (Lymantria dispar) caterpillars prefer to feed on black poplar (Populus nigra) foliage infected by the rust fungus Melampsora larici‐populina instead of uninfected control foliage, and selectively consume fungal spores. This consumption, also observed in a related lepidopteran species, is stimulated by the sugar alcohol mannitol, found in much higher concentration in fungal tissue and infected leaves than uninfected plant foliage. Gypsy moth larvae developed more rapidly on rust‐infected leaves, which cannot be attributed to mannitol but rather to greater levels of total nitrogen, essential amino acids and B vitamins in fungal tissue and fungus‐infected leaves. Herbivore consumption of fungi and other microbes may be much more widespread than commonly believed with important consequences for the ecology and evolution of plant–herbivore interactions.

show abstract

“…B vitamins were analysed from the extract by LC‐MS/MS using a method described by Khaksari et.al. (2018) with modifications (Table S3).…”

Section: Methodsmentioning

confidence: 99%

Herbivory meets fungivory: insect herbivores feed on plant pathogenic fungi for their own benefit

et al. 2020

View full text Add to dashboard Cite

show abstract

“…However, all of these methods are not confirmatory approaches, because they cannot provide direct proof of the analytes structures. Undoubtedly, the introduction of liquid chromatography-mass spectrometry (LC-MS) [16] and gas chromatography-mass spectrometry (GC-MS) [17] was a major step towards analysis of multivitamins in different matrices. The obvious advantage of MS is that it is a global quantum detector of any compound.…”

Section: Introductionmentioning

confidence: 99%

Dilute-and-shoot-based direct nano-electrospray ionization tandem mass spectrometry as screening methodology for multivitamins in dietary supplement and human urine

Amer

Zarad

El-Gendy

et al. 2020

Journal of Advanced Research

View full text Add to dashboard Cite

show abstract

Biological Properties of Vitamins of the B-Complex, Part 1: Vitamins B1, B2, B3, and B5

et al. 2022

View full text Add to dashboard Cite

This review summarizes the current knowledge on essential vitamins B1, B2, B3, and B5. These B-complex vitamins must be taken from diet, with the exception of vitamin B3, that can also be synthetized from amino acid tryptophan. All of these vitamins are water soluble, which determines their main properties, namely: they are partly lost when food is washed or boiled since they migrate to the water; the requirement of membrane transporters for their permeation into the cells; and their safety since any excess is rapidly eliminated via the kidney. The therapeutic use of B-complex vitamins is mostly limited to hypovitaminoses or similar conditions, but, as they are generally very safe, they have also been examined in other pathological conditions. Nicotinic acid, a form of vitamin B3, is the only exception because it is a known hypolipidemic agent in gram doses. The article also sums up: (i) the current methods for detection of the vitamins of the B-complex in biological fluids; (ii) the food and other sources of these vitamins including the effect of common processing and storage methods on their content; and (iii) their physiological function.

show abstract

Detection and quantification of vitamins in microliter volumes of biological samples by LC‐MS for clinical screening

Cited by 3 publications

References 42 publications

Herbivory meets fungivory: insect herbivores feed on plant pathogenic fungi for their own benefit

Herbivory meets fungivory: insect herbivores feed on plant pathogenic fungi for their own benefit

Dilute-and-shoot-based direct nano-electrospray ionization tandem mass spectrometry as screening methodology for multivitamins in dietary supplement and human urine

Biological Properties of Vitamins of the B-Complex, Part 1: Vitamins B1, B2, B3, and B5

Contact Info

Product

Resources

About