Detection and quantification of toxins in cultures of microcystis aeruginosa (pcc 7820) by hplc and protein phosphatase inhibition assayffect of blending various collectors at bulk

Abstract

ABSTRACT:-Increasing anthropogenic eutrophication in lakes, drinking water reservoirs and coastal waters is a world-wide phenomenon leading to the formation of blooms of toxic cyanobacteria. These pose a significant threat to human and animal health hence the need for sensitive methods for their detection, identification and quantification. This report presents two methods: analytical high power liquid chromatography coupled with photo-diode array detection and protein phosphatase inhibition assay for the analysis of the most frequently encountered cyanobacterial hepatotoxinsthe microcystins. Four microcystin variants: microcystin -LR, -LY, -LW and -LF were identified and quantified by HPLC in cells and growth media of cultured Microcystis aeruginosa PCC 7820. The protein phosphatase inhibition assay was used to estimate potential toxicity of cyanobacterial extracts and both methods showed good correlation (R2 = 0.91). Although HPLC provides accurate and specific information on the identity and quantity of each microcystin variant, it is quite expensive.The assay method on the other hand is relatively cheaper and can be modified to measure milligramme quantities of sample on a benchtop spectrophotometer but individual microcystin variants cannot be identified

Evaluation of phytotoxicity and ecotoxicity potentials of a cyanobacterial extract containing microcystins under realistic environmental concentrations and in a soil–plant system

“…Therefore, the toxic potential and the microcystin patterns of this strain are in accordance with those reported by several previous studies on the same strain (Akin-Oriola and Lawton, 2005;Ríos et al, 2013), where 4 major MC variants (MC-LR, -LF, -LW, and -LY) and 2-6 minor variants were identified.…”

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“…Silver Nanoparticles: To prepare silver nanoparticles, 5 ml of microcystin containing algal extract was mixed with 45 ml of 1 mM AgNO 3 solution and incubated at 24°C with constant stirring at 120 rpm 15 . The formation of nanoparticles was detected by monitoring the change in color of the solution using a UV-visible spectrophotometer (Shimadzu UV-1800).…”