Detection and Quantification of Macrolide Resistance Mutations at Positions 2058 and 2059 of the 23S rRNA Gene by Pyrosequencing

Haanperä, Marjo; Huovinen, Pentti; Jalava, Jari

doi:10.1128/aac.49.1.457-460.2005

Cited by 60 publications

(42 citation statements)

References 15 publications

(15 reference statements)

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“…Since its development in the late 1990s, pyrosequencing has been used extensively in several fields, including chromatin research and plant biology (228,246). In microbiology, pyrosequencing has allowed for rapid sequencing of whole genomes, comparisons of multiple strains of bacteria, and quick detection of point mutations responsible for antibiotic resistance, among other advances (59,121,227,322,333).…”

Section: Microbial Ecology: a Brief Historymentioning

confidence: 99%

From Structure to Function: the Ecology of Host-Associated Microbial Communities

Robinson

Bohannan

Young

2010

Microbiol Mol Biol Rev

356

310

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SUMMARY In the past several years, we have witnessed an increased interest in understanding the structure and function of the indigenous microbiota that inhabits the human body. It is hoped that this will yield novel insight into the role of these complex microbial communities in human health and disease. What is less appreciated is that this recent activity owes a great deal to the pioneering efforts of microbial ecologists who have been studying communities in non-host-associated environments. Interactions between environmental microbiologists and human microbiota researchers have already contributed to advances in our understanding of the human microbiome. We review the work that has led to these recent advances and illustrate some of the possible future directions for continued collaboration between these groups of researchers. We discuss how the application of ecological theory to the human-associated microbiota can lead us past descriptions of community structure and toward an understanding of the functions of the human microbiota. Such an approach may lead to a shift in the prevention and treatment of human diseases that involves conservation or restoration of the normal community structure and function of the host-associated microbiota.

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Section: Microbial Ecology: a Brief Historymentioning

confidence: 99%

From Structure to Function: the Ecology of Host-Associated Microbial Communities

Robinson

Bohannan

Young

2010

Microbiol Mol Biol Rev

356

310

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“…[3][4][5]. However, there is little information available on the characteristics of the resistance mechanism of EM-resistant C. coli isolates from food-producing animals in Japan.…”

mentioning

confidence: 99%

Characterization of Macrolide-Resistant Campylobacter coli Isolates from Food-Producing Animals on Farms Across Japan during 2004

Harada

Asai

Kojima

et al. 2006

The Journal of Veterinary Medical Science

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ABSTRACT. We investigated the susceptibilities against 7 antimicrobial agents in Campylobacter jejuni and C. coli isolates from food-producing animals in 2004. In comparison with the results of past surveillance, no significant difference was observed in resistance rates against all of the antimicrobials tested in Campylobacter isolates. However, slight increase of erythromycin (EM) resistance was found in C. coli isolates from pigs. We examined the mutation of the 23S rRNA gene and their susceptibilities against azithromycin, tylosin, and lincomycin in 44 EM-resistant isolates and 28 susceptible isolates of porcine origin. All the EM-resistant isolates contained A2075G in the 23S rRNA gene and showed cross-resistance to azithromycin, tylosin, and lyncomycin.

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“…Pyrosequencing is a reliable technique that allows fast identification of short DNA sequences. It has already been used as a rapid and convenient approach for detection of antibiotic resistances, such as ciprofloxacin resistance in Neisseria gonorrhoeae, rifampin resistance in Mycobacterium tuberculosis, macrolide resistance in Streptococcus pneumoniae, Streptococcus pyogenes, Mycobacterium avium, Campylobacter jejuni, and Haemophilus influenzae, and linezolid resistance in enterococci (4,6,15,16,23).…”

mentioning

confidence: 99%

Pyrosequencing as a Rapid Tool for Identification of GES-Type Extended-Spectrum β-Lactamases

2006

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A pyrosequencing technique was used for identification of extended-spectrum ␤-lactamases (ESBLs) of GES type. These ␤-lactamases are isolated increasingly emerging in gram-negative bacteria worldwide. This rapid and reliable identification method is interesting, since GES variants, including not only expanded-spectrum cephalosporins but also carbapenems, cephamycins, and monobactams, are the only ESBLs that possess different hydrolysis profiles.

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Detection and Quantification of Macrolide Resistance Mutations at Positions 2058 and 2059 of the 23S rRNA Gene by Pyrosequencing

Cited by 60 publications

References 15 publications

From Structure to Function: the Ecology of Host-Associated Microbial Communities

From Structure to Function: the Ecology of Host-Associated Microbial Communities

Characterization of Macrolide-Resistant Campylobacter coli Isolates from Food-Producing Animals on Farms Across Japan during 2004

Pyrosequencing as a Rapid Tool for Identification of GES-Type Extended-Spectrum β-Lactamases

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