Detection and quantification of lipid membrane binding on silica micro-tube resonator sensor

Ling, Tao; Majd, Sheereen; Mayer, Michael; Guo, Lufang

doi:10.1117/12.763091

Cited by 5 publications

(2 citation statements)

References 9 publications

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“…Due to this limitation, most biophysical and biochemical studies on giant liposomes so far have been carried out in aqueous solutions with an ionic strength significantly below the physiologic range. , At least six important properties of giant liposomes can, however, be affected by ionic strength; these include: (i) electrostatic interactions of lipid membranes with proteins, − with adjacent lipid membranes, , or with other molecules or ions in solution; − (ii) osmotic properties; ,,, (iii) packing of lipid headgroups in the membrane; , (iv) curvature, bending elasticity, and mechanical stability of lipid bilayers; , (v) activity of membrane proteins; and (vi) ion channel conductance. − Consequently, it would be desirable to prepare giant liposomes in solutions that make it possible to extend these studies to physiologically relevant salt concentrations …”

Section: Introductionmentioning

confidence: 99%

Films of Agarose Enable Rapid Formation of Giant Liposomes in Solutions of Physiologic Ionic Strength

et al. 2009

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This paper describes a method to form giant liposomes in solutions of physiologic ionic strength, such as phosphate buffered saline (PBS) or 150 mM KCl. Formation of these cell-sized liposomes proceeded from hybrid films of partially dried agarose and lipids. Hydrating the films of agarose and lipids in aqueous salt solutions resulted in swelling and partial dissolution of the hybrid films and in concomitant rapid formation of giant liposomes in high yield. This method did not require the presence of an electric field or specialized lipids; it generated giant liposomes from pure phosphatidylcholine lipids or from lipid mixtures that contained cholesterol or negatively charged lipids. Hybrid films of agarose and lipids even enabled the formation of giant liposomes in PBS from lipid compositions that are typically problematic for liposome formation, such as pure phosphatidylserine, pure phosphatidylglycerol, and asolectin. This paper discusses biophysical aspects of the formation of giant liposomes from hybrid films of agarose and lipids in comparison to established methods and shows that gentle hydration of hybrid films of agarose and lipids is a simple, rapid, and reproducible procedure to generate giant liposomes of various lipid compositions in solutions of physiologic ionic strength without the need for specialized equipment.

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Section: Introductionmentioning

confidence: 99%

Films of Agarose Enable Rapid Formation of Giant Liposomes in Solutions of Physiologic Ionic Strength

et al. 2009

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“…These optical microresonators have been manufactured with different morphologies in the form of capillaries [1][2][3][4], discs [5], rings [6 -8], toroids [9], spheres [10], bubbles [11], bottles [12], etc, using different materials such as silicon, silica, polymers, with applications in areas such as biology [13], medicine [4], physics [14], chemistry [10] and specifically in the area of sensors for the measurement of temperature [15], humidity [12], refractive index [16] and some other physical variables of interest [6]. Specifically, the cylindrical optical microcavities that have been made using different types of materials, experience a series of resonances commonly known as Whispering Gallery modes WGMs, which are characteristic of the cavities that have rotational symmetry and can be explained through the phenomenon of total internal reflection occurring within these cavities when they are excited through an external source.…”

Section: Introductionmentioning

confidence: 99%

Sensitivity of an optical sensor based on capillary microresonator for the measurement the hydrostatic pressure in microfluidics

Padilla¹,

Piñeres²,

Torres³

2018

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Abstract. In this investigation, we report the study of an optical device for the measurement of hydrostatic pressure in fluids. The device studied is a sensor based on a dielectric optical resonator in the form of a capillary that confines the light in its interior through the phenomenon of total internal reflection. In the analytical study of the sensor, the excitation of the azimuthal modes WGMs inside the resonant cavity is considered, so that their sensitivity to changes in the hydrostatic pressure was analyzed as a function of the displacement of wavelengths of resonances in the cavity.

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Capillary resonator as optical sensor for the measurement of the hydrostatic pressure. A theoretical study of its sensitivity

Avila

Horta

Torres

2019

J. Phys.: Conf. Ser.

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We report the study of an optical device for the measurement of hydrostatic pressure in fluids. The device studied is a sensor based on a dielectric optical resonator in the form of a capillary that confines the light in its interior through the phenomenon of total internal reflection. For the analytical study of sensitivity, we have considered the solution of the Helmholtz scalar equation for the case of a resonant cavity in form of cylinder composed of three layers with different refractive index. During the study, the excitation of the Whispering Gallery Modes WGMs on a transverse plane along the axial axis of the cavity is studied and the equation of eigenvalues that has information of the wavelengths of resonances for the TE and TM modes were obtained from the resonance conditions. To determine the sensitivity of the device, the shift of the wavelengths of resonances as a function of the internal pressure in the cavity were analyzed. The results show that it is possible to increase the sensitivity of the microresonator when the wall thickness of the cavity is thin, and the maximum diameter is increased.

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Detection and quantification of lipid membrane binding on silica micro-tube resonator sensor

Cited by 5 publications

References 9 publications

Films of Agarose Enable Rapid Formation of Giant Liposomes in Solutions of Physiologic Ionic Strength

Films of Agarose Enable Rapid Formation of Giant Liposomes in Solutions of Physiologic Ionic Strength

Sensitivity of an optical sensor based on capillary microresonator for the measurement the hydrostatic pressure in microfluidics

Capillary resonator as optical sensor for the measurement of the hydrostatic pressure. A theoretical study of its sensitivity

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