Detection and quantification of <i>Aeromonas schubertii</i> in <i>Channa maculata</i> by TaqMan <scp>MGB</scp> probe fluorescence real‐time quantitative <scp>PCR</scp>

Lei, Chun; Guo, Yanming M.; Cao, Jizhen; Zhang, Defeng; Chang, Ouqin; Li, Kaibin; Fang, Wang; Shi, Chao; Jiang, Lan; Wang, Qing; Lin, Li

doi:10.1111/jfd.12911

Cited by 13 publications

(6 citation statements)

References 47 publications

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“…(Figure 3 A). 9,15,16 Using samples from fur swabs, the MC28S qPCR assay correctly identified all 10 mice in groups 1 and 3 as positive for M. musculinus and all 5 group 2 samples as negative (Figure 3 B, Table 3).…”

Section: Resultsmentioning

confidence: 98%

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Detection of Myocoptes musculinus in Fur Swab and Fecal Samples by Using PCR Analysis

Lee

Shen

Holcombe

et al. 2019

j am assoc lab anim sci

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Current methods for detecting mites in mouse colonies have limitations in terms of cost, accuracy, and throughput. To address these limitations, we developed PCR assays to detect Myocoptes musculinus in fecal samples. Using a newly generated ribosomal RNA sequence of M. musculinus (MC28S), we developed PCR and qPCR assays capable of detecting M. musculinus mites or eggs ingested during grooming. To determine our ability to detect mites, we tested fur swabs and feces from mouse colonies experimentally infested with M. musculinus and Demodex musculi, 2) Myobia musculi and Radfordia affinis, 3) M. musculinus and M. musculi, and 4) no mites (negative control). The MC28S PCR and qPCR assays positively identified M. musculinus in groups 1 and 3. The MC28S PCR assay detected M. musculinus in 9 of 10 fecal samples from known-positive animals, whereas the qPCR assay correctly identified M. musculinus in all 10 fecal samples. To our knowledge, this report is the first description of PCR-based detection of murine mites in feces. By eliminating the need for pelt examinations, mite detection from fecal samples can facilitate mite detection in sentinel or quarantine programs.

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Section: Resultsmentioning

confidence: 98%

“…To establish the limit of detection, the lowest dilution level at which all 8 replicates were positive was determined. 9,15,16 Linear regression analysis was performed by using Excel 2016 (Microsoft, Redmond, WA); the correlation coefficient was calculated by using the CORREL function, and the amplification efficiency was computed as 10 (-1/slope) -1.…”

mentioning

confidence: 99%

Detection of Myocoptes musculinus in Fur Swab and Fecal Samples by Using PCR Analysis

Lee

Shen

Holcombe

et al. 2019

j am assoc lab anim sci

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“…agalactiae (TFJ‐ery), from the natural low‐virulence S . agalactiae strain TFJ0901 as basis, gave almost 100% protection of tilapia 40 …”

Section: Reviewmentioning

confidence: 99%

“…gyrB , rpoB , ela and dnaJ ) has been used for bacterial identification 261,262 . Recently, Liu et al 40 reported a highly sensitive TaqMan MGB probe fluorescence real‐time quantitative PCR for detecting and quantifying A . schubertii from snakehead fish.…”

Section: Reviewmentioning

confidence: 99%

Bacterial diseases of tilapia, their zoonotic potential and risk of antimicrobial resistance

Haenen

Dong

Hoai

et al. 2023

Reviews in Aquaculture

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Tilapia culture is an important source of income and nutrition to many rural families. Since 2000, the production of tilapia increased and reached domestic and global markets. Major farmed species is Nile tilapia (Oreochromis niloticus), in earthen ponds and cage cultures. Intensification contributed to global tilapia disease outbreaks, with bacterial infections causing mortalities and morbidities, threatening sustainable production. At tilapia farms, high nutrient concentrations, water temperature and fish densities enhance bacterial growth including virulent bacterial clones and potential zoonotic bacteria. Global warming favours this. This review respectively provides a comprehensive overview of the most common and emerging bacterial pathogens, diseases, clinical presentations and diagnostics of tilapia, including bacteria and diseases with zoonotic potential. First, common bacterial disease outbreaks, including streptococcosis, motile Aeromonas septicaemia, francisellosis, columnaris disease and vibriosis are described. Then, information on emerging bacterial infections of concern for tilapia, like edwardsiellosis through Edwardsiella ictaluri and E. tarda, as well as Aeromonas schubertii is provided. Reports of infectious bacterial tilapia disease outbreaks from other bacteria, including Lactococcus garvieae, Aerococcus viridans, Pseudomonas spp., Mycobacterium marinum and Chlamydia spp., and others are reviewed. Furthermore, bacteria with zoonotic potential, like Streptococcus agalactiae ST283, S. iniae, Aeromonas sp., E. tarda, Vibrio vulnificus pathovar (pv) piscis and M. marinum are included in the review, to provide the most current overview of the disease risks affecting production and post‐harvest stages. Additionally, the status and risks of antimicrobial resistance in bacteria from tilapia and other cultured fish through imprudent use of antibiotics, and its future at a global level are provided.

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“…Transcriptome profiling analysis has also been used for Epinephelus coioides [ 15 ], Cynoglossus semilaevis [ 16 ], and Ctenopharyngodon idella [ 17 ]. Although white spots disease, caused by A. schubertii , is one of the most serious diseases in snakehead fish [ 18 ], few reports have considered the host immune response to this pathogen during infection.…”

Section: Introductionmentioning

confidence: 99%

Immune Response and Apoptosis-Related Pathways Induced by Aeromonas schubertii Infection of Hybrid Snakehead (Channa maculata♀ × Channa argus♂)

Liu

Zhang

et al. 2021

Pathogens

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Aeromonas schubertii is the etiological pathogen of internal organ nodules in snakehead fish. Infections with A. schubertii produce a significant economic loss in aquaculture. Therefore, it is important to examine the immune mechanisms by which snakeheads defend against A. schubertii infection. In this study, we established a hybrid snakehead infection model by intraperitoneal injection of A. schubertii that produced internal organ nodules. The splenic immune response of infected fish was examined at the transcriptome level by Illumina-seq analysis. Results showed 14,796 differentially expressed genes (DEGs) following A. schubertii infection, including 4441 up-regulated unigenes and 10,355 down-regulated unigenes. KEGG analysis showed 2084 DEGs to be involved in 192 pathways, 14 of which were immune-related. Twelve DEGs were used to validate quantitative real-time PCR results with RNA-seq data. Time-course expression analysis of six genes demonstrated modulation of the snakehead immune response by A. schubertii. Furthermore, transcriptome analysis identified a substantial number of DEGs that were involved in the apoptosis signaling pathway. TUNEL analysis of infected spleens confirmed the presence of apoptotic cells. This study provided new information for a further understanding of the pathogenesis of A. schubertii in snakeheads, which can be used to prevent and possibly treat A. schubertii infections.

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Detection and quantification of Aeromonas schubertii in Channa maculata by TaqMan MGB probe fluorescence real‐time quantitative PCR

Cited by 13 publications

References 47 publications

Detection of Myocoptes musculinus in Fur Swab and Fecal Samples by Using PCR Analysis

Detection of Myocoptes musculinus in Fur Swab and Fecal Samples by Using PCR Analysis

Bacterial diseases of tilapia, their zoonotic potential and risk of antimicrobial resistance

Immune Response and Apoptosis-Related Pathways Induced by Aeromonas schubertii Infection of Hybrid Snakehead (Channa maculata♀ × Channa argus♂)

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