Detection and Quantification of Cytokines and Other Biomarkers

Chiswick, Evan L.; Duffy, Elizabeth; Japp, B.; Remick, Daniel G.

doi:10.1007/978-1-61779-527-5_2

Cited by 75 publications

(51 citation statements)

References 12 publications

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“…Images were captured using Quansys Imager, driven by an 8.4 megapixel Canon 20D digital SLR camera, and analyzed using Quansys Software. In order to assure compatibility with measurements of cytokines in previously published studies in the field (Chiswick et al, 2012, Trune et al, 2011, Wong et al, 2008), the antigen standard concentrations used by Quansys (R&D) were referenced to “gold standard” for each cytokine represented on the multiplex plate as previously described (Lattie, Antoni, 2012). …”

Section: Methodsmentioning

confidence: 99%

Poor sleep quality is associated with greater circulating pro-inflammatory cytokines and severity and frequency of chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME) symptoms in women

Milrad

Hall

Lattie

et al. 2017

Journal of Neuroimmunology

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Objective Poor sleep quality has been linked to inflammatory processes and worse disease outcomes in the context of many chronic illnesses, but less is known in conditions such as chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME). This study examines the relationships between sleep quality, pro-inflammatory cytokines, and CFS/ME symptoms. Methods Sixty women diagnosed with CFS/ME were assessed using the Pittsburgh Sleep Quality Index (PSQI), Fatigue Symptom Inventory (FSI) and Center for Disease Control and Prevention (CDC)-based CFS/ME symptom questionnaires. Circulating plasma pro-inflammatory cytokine levels were measured by ELISA. Multiple regression analyses examined associations between sleep, cytokines and symptoms, controlling for age, education, and body mass index. Results Poor sleep quality (PSQI global score) was associated with greater pro-inflammatory cytokine levels: interleukin-1β (IL-1β) (β=0.258, p=0.043), IL-6 (β=0.281, p=0.033), and tumor necrosis factor-alpha (TNF-α) (β=0.263, p=0.044). Worse sleep quality related to greater fatigue severity (β=0.395, p=0.003) and fatigue-related interference with daily activities (β=0.464, p<0.001), and more severe and frequent CDC-defined core CFS/ME symptoms (β=0.499, p<0.001, and β=0.556, p<0.001, respectively). Conclusions Results underscore the importance of managing sleep-related difficulties in this patient population. Further research is needed to identify the etiology of sleep disruptions in CFS/ME and mechanistic factors linking sleep quality to symptom severity and inflammatory processes.

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Section: Methodsmentioning

confidence: 99%

Poor sleep quality is associated with greater circulating pro-inflammatory cytokines and severity and frequency of chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME) symptoms in women

Milrad

Hall

Lattie

et al. 2017

Journal of Neuroimmunology

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“…The most common approach for cytokine quantification is based on the idea of an immunoassay. Specific techniques include traditional ELISA assays, (Chiswick et al 2012) enzyme-linked immunosorbent spot (ELIspot) assays, (Cox et al 2006) antibody array assays (Schröder et al 2010) and bead-based assays (Won et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Recent advances in cytokine detection by immunosensing

Liu

Meng

Hutchinson

et al. 2016

Biosensors and Bioelectronics

116

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The detection of cytokines in body fluids, cells, tissues and organisms continues to attract considerable attention due to the importance of these key cell signaling molecules in biology and medicine. In this review, we describe recent advances in cytokine detection in the course of ongoing pursuit of new analytical approaches for these trace analytes with specific focus on immunosensing. We discuss recent elegant designs of sensing interface with improved performance with respect to sensitivity, selectivity, stability, simplicity, and the absence of sample matrix effects. Various immunosensing approaches based on multifunctional nanomaterials open novel opportunities for ultrasensitive detection of cytokines in body fluids in vitro and in vivo. Methodologies such as suspension arrays also known as bead assays together with optical fiber-based sensors, on their own or in combination with microfluidic devices will continue to have an important role to address the grand challenge of real-time in vivo multiplex cytokine detection.

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“…[30] Proinflammatory cytokines, particularly interleukin 1 (IL-1) and tumor necrosis factor alpha (TNF-b) upregulated during the inflammatory process. [31] The standard enzyme-linked immunosorbent assay method was used for analyzing quantification of FGF, TGF-b1, IL-1 alpha (IL-1a), VEGF, TNF-a, plateletderived growth factor (PDGF) and caspase 3 (CASP3) cytokines [32] to evaluate the effect of MSCs in the infected tissue samples.…”

Section: Methodsmentioning

confidence: 99%

Mesenchymal stem cells have significant anti-infective effect on methicillin-resistant Staphylococcus epidermidis vascular graft infections

Canbeyli

Kabalcı

Çirpar

et al. 2019

Eklem Hastalik Cerrahisi

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ÖZ Amaç: Bu çalışmada, mezenkimal kök hücre (MKH) implantasyonunun metisiline dirençli Stafilokokus epidermidis (MDSE)'in neden olduğu vasküler greft enfeksiyonları üzerindeki etkileri değerlendirildi ve antibiyotik tedavisi ile karşılaştırıldı. Gereç ve yöntemler: Sağlıklı erişkin 56 Wistar sıçanı (yaş, 5 ay üzeri; ağırlık, 300-350 g) sekiz gruba ayrıldı. Grup 1 kontrol grubu, grup 2 enfekte kontrol grubu olarak tanımlandı. Grup 3 ve 4 Dakron grefti uygulanan, MDSE ile enfekte olan, sırasıyla tigesiklin ve MKH tedavisi uygulanan gruplar olarak tanımlandı. Grup 5 ve 6 politetrafloroetilen (PTFE) greft uygulanarak MDSE ile enfekte edildi. Bu gruplara da sırasıyla tigesiklin ve MKH tedavisi uygulandı. Grup 7 ve 8 greft uygulanmadan, MDSE ile enfekte edildi ve sırasıyla bu gruplara da tigesiklin ve MKH tedavisi uygulandı. Greftler ve yumuşak doku örnekleri ameliyattan 13 gün sonra alındı. Greft çevresi dokuda koloni sayımı yapıldı. Tüm numuneler, kök hücre aktivitesini gösteren belirteçler açısından enzime bağlı bağışıklık deneyi (ELISA) ile değerlendirildi. Bulgular: Tedavilerin genel başarısı, kullanılan greftten bağımsız olarak, MDSE rekürrensi olan sıçanların sayısı ile değerlendirildi. Tedavi edilmeyen grup 2, tigesiklin grupları (3, 5 ve 7) ile MKH grupları (4, 6 ve 8) arasındaki farklılık istatistiksel olarak anlamlıydı. Mezenkimal kök hücre ve tigesiklin tedavilerinin başarısı Dakron, PTFE ve greft uygulanmayan gruplarda benzerdi. Dakron gruplarında MDSE enfeksiyonunun hem MKH hem de tigesiklin tedavisine karşı bir direnci vardı. Bu, Dakron greftlerinin enfeksiyona duyarlılığının bir göstergesi olarak kabul edildi. Ancak, grup 2 ve Dakron grupları arasında bakteriyel kolonizasyon açısından anlamlı farklılık yoktu. ELISA sonuçları üç sitokinde anlamlıydı. Sonuç: Mezenkimal kök hücreler, tek başına alternatif bir tedavi seçeneği veya vasküler greft enfeksiyonlarının kontrolü için kombine terapinin bir parçası olarak kabul edilebilir. Anahtar sözcükler: Dakron, mezenkimal kök hücreler, politetrafloroetilen, tigesiklin, vasküler greft enfeksiyonları. ABSTRACTObjectives: This study aims to evaluate the effects of mesenchymal stem cell (MSC) implantation on vascular graft infections caused by methicillinresistant Staphylococcus epidermidis (MRSE) and compare with antibiotic treatment. Materials and methods:Healthy adult 56 Wistar rats (age, over 5 months; weighing, 300-350 g) were divided into eight groups. Group 1 was defined as the control group and group 2 was defined as the infected control group. Groups 3 and 4 were defined as Dacron grafted and MRSE infected groups, treated with tigecycline and MSCs, respectively. Groups 5 and 6 were performed polytetrafluoroethylene (PTFE) graft and infected with MRSE. These groups were also administered tigecycline and MSC treatment, respectively. Groups 7 and 8 were infected with MRSE without graft administration and were also performed tigecycline and MSC treatment, respectively. Grafts and soft tissue specimens were collected at 13 days postoperatively. Colony counts of...

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Detection and Quantification of Cytokines and Other Biomarkers

Cited by 75 publications

References 12 publications

Poor sleep quality is associated with greater circulating pro-inflammatory cytokines and severity and frequency of chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME) symptoms in women

Poor sleep quality is associated with greater circulating pro-inflammatory cytokines and severity and frequency of chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME) symptoms in women

Recent advances in cytokine detection by immunosensing

Mesenchymal stem cells have significant anti-infective effect on methicillin-resistant Staphylococcus epidermidis vascular graft infections

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