Detection and phylogeny of Lymphocystivirus in sea bream Sparus aurata based on the DNA polymerase gene and major capsid protein sequences

Kvitt, Hagit; Heinisch, Gilad; Diamant, A.

doi:10.1016/j.aquaculture.2008.01.007

Cited by 43 publications

(40 citation statements)

References 13 publications

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“…Genotype VII is a new genotype. These results are consistent with the results of Yan et al [3] and Hossain et al [15], but do not support the conclusions of Kitamura et al [14] and Kvitt et al [16]. From an evolutionary point of view, LCDV1 differentiated first, followed by LCDV-rf, LCDV-tl, LCDV-cb and LCDV-sa, and then by LCDV-rc and LCDV-sb, LCDV-cn, LCDV-C, and LCDV-jf.…”

Section: Discussionsupporting

confidence: 85%

“…The mcp gene is one of the most important molecular markers for analyzing the genetic relationships among iridoviruses because its nucleotide sequence is relatively conserved within the Iridoviridae family [12,13]. Recently, several LCDV genotypes were reported, based on the mcp gene and the pathogenicity of the virus [3,[14][15][16][17]. According to the results of these studies, we speculated that the genetic similarity of LCDVs may be related to their host fish species and studies into the evolution and genetic similarities of LCDVs should take into consideration the evolutionary history of their host fish species.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of the genetic diversity of the lymphocystis virus and its evolutionary relationship with its hosts

Yan

Jian

et al. 2011

Virus Genes

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Lymphocystis disease virus (LCDV) is the causative agent of lymphocystis disease. In this study, the mcp gene of LCDV and the cyt b gene of the host fish were selected as molecular markers, and the phylogenetic relationships between LCDV and its host were analyzed. The 25 LCDV isolates examined in this study were attributed to seven LCDV genotypes: genotype I (LCDV-1), genotype II (LCDV-cn, etc.), genotype III (LCDV-rf), genotype IV (LCDV-rc and LCDV-sb), genotype V (LCDV-cb), genotype VI (LCDV-tl), and genotype VII (LCDV-sa). Genotype VII is a new genotype. LCDV1 was found to have differentiated first, followed by LCDV-rf; then LCDV-tl; LCDV-cb; and then LCDV-sa; and by LCDV-rc and LCDV-sb; and finally by LCDV-cn, LCDV-C, and LCDV-jf. From the host evolutionary perspective, Rachycentron canadum was found to have differentiated first, followed by Trichogaster leeri, Chanda baculis, and Sebastes schlegeli, Lateolabrax sp., Sparus aurata, Platichthys flesus, and Paralichthys olivaceus. Comparison of the phylogenies of the host fish species and LCDVs revealed no significant evidence of cospeciation between LCDVs and their host fish. In-depth studies of the genetic variation in LCDVs can enhance our understanding of the mechanism of LCDV infection, which may provide important insights into the prevention and treatment of lymphocystis disease.

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Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Analysis of the genetic diversity of the lymphocystis virus and its evolutionary relationship with its hosts

Yan

Jian

et al. 2011

Virus Genes

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“…Gill, stomach, intestine, skin, kidney, spleen and liver are known to be the target tissues of LCDV in fish (Colorni & Diamant 1995, Kvitt et al 2008, Zhan et al 2010. In order to investigate the distribution of LCDV receptor-27.8 kDa in flounder, we extracted proteins from the target tissues of LCDV and performed western blotting.…”

Section: Discussionmentioning

confidence: 99%

Monoclonal antibodies against 27.8 kDa protein receptor efficiently block lymphocystis disease virus infection in flounder Paralichthys olivaceus gill cells

Sheng

Wang²,

Xing³

et al. 2012

Dis. Aquat. Org.

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“…; Kvitt et al . ). In addition, water contamination, oxygen depletion and nutrition deficiency in fish culture may activate virus latent in asymptomatic carriers and lead to a disease occurrence (Cano et al .…”

Section: Introductionmentioning

confidence: 97%

Development of a colloidal gold immunochromatographic test strip for detection of lymphocystis disease virus in fish

2012

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Aims: To develop a gold immunochromatographic test strip for on-site rapid detection of lymphocystis disease virus (LCDV). Methods and Results: Monodispersional colloidal gold and gold-labelled anti-LCDV monoclonal antibody (McAb) 2D11 were prepared and characterized by UV-visible spectroscopy and transmission electron microscopy. Gold-labelled probe was used as the detection antibody, and goat anti-mouse IgG at the control line and anti-LCDV McAb 1A8 at the test line of the test strip served as the capture antibody. The positive results could be easily judged by the presence of a red test line with naked eye within 10 min. The test strip, in good agreement with enzyme-linked immunosorbent assay and dot-blotting in sensitivity and LCDV detection, gave a detection limit of 1 lg ml À1 of LCDV and was stable for 6 months at room temperature and 12 months at 4°C. Conclusions: The test strip was specific, simple and convenient for rapid detection of LCDV presenting good stability and reproducibility. Significance and Impact of the Study: This ready-to-use test strip allows on-site rapid detection of LCDV in fish without the requirement of specialized equipments and professional personnel, which could augment the practical application for diagnosis of LCDV even in disadvantage areas.

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Detection and phylogeny of Lymphocystivirus in sea bream Sparus aurata based on the DNA polymerase gene and major capsid protein sequences

Cited by 43 publications

References 13 publications

Analysis of the genetic diversity of the lymphocystis virus and its evolutionary relationship with its hosts

Analysis of the genetic diversity of the lymphocystis virus and its evolutionary relationship with its hosts

Monoclonal antibodies against 27.8 kDa protein receptor efficiently block lymphocystis disease virus infection in flounder Paralichthys olivaceus gill cells

Development of a colloidal gold immunochromatographic test strip for detection of lymphocystis disease virus in fish

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