Detection and phylogenetic analysis of Orf virus from sheep in Brazil: a case report

Abrahão, Jônatas Santos; Campos, Rafael K.; Trindade, Giliane S.; Guedes, M.I.M.C.; Lobato, Zélia Inês Portela; Mazur, Carlos; Ferreira, P. C. P.; Bonjardim, Cláudio Antônio; Kroon, Erna Geessien

doi:10.1186/1743-422x-6-47

Cited by 42 publications

(42 citation statements)

References 14 publications

(19 reference statements)

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“…ORFVs from the same outbreak have been reported [1,5,6,12,14,24]. Guo et al reported an outbreak of ORFV infection in a zoo, in which a single ORFV likely infected musk ox (Ovibos moschatus), domestic Shetland sheep (Ovis aries) and Sichuan takin (Budorcas taxicolor tibetana), but the amino acid sequence of the viral envelope gene contained one or two substitutions in each animal [12].…”

Section: Discussionmentioning

confidence: 99%

“…Sequence analyses of the viral envelope gene indicate that contagious pustular dermatitis in Japanese serows is mainly caused by ORFV [18,19]. However, only a partial sequence of the envelope gene was analyzed in these studies, and in other countries, sequence variations occur in the envelope gene of ORFVs isolated from the same outbreaks [1,5,6,12,14,24]. In general, the amino acid sequences of the genes encoding viral structural proteins, such as the envelope, are relatively well conserved, but the detailed genetic relationship between the PPVs of the recent and earlier outbreaks in Japanese serows is unclear.…”

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confidence: 99%

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Spatial and Temporal Genetic Homogeneity of Orf Viruses Infecting Japanese Serows (Capricornis crispus)

Inoshima

Ito

Ishiguro

2010

The Journal of Veterinary Medical Science

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ABSTRACT. This study genetically characterized orf viruses (ORFVs) isolated from recent outbreaks in Japanese serows (Capricornis crispus) in 2007 and 2008, and from earlier outbreaks from 1985 to 2001. Nucleotide sequences of genes for the viral envelope, vascular endothelial growth factor (VEGF), and virus interferon resistance (VIR) were determined in two ORFVs isolated from recent outbreaks and in eight from earlier outbreaks. No deletions or insertions were observed in these genes. Surprisingly, the amino acid sequences of the envelope and VIR genes were identical among the 10 ORFVs, and only one amino acid substitution in the VEGF gene was found in one of the two recent ORFVs (2007). Genotyping by differential PCR for the A32L gene, which encodes an ATPase, classified all of the 10 ORFVs into the same genotype. In an ORFV isolated from a sheep in 1970, the three sequenced genes were almost the same as in the ORFVs isolated from Japanese serows, and the ORFV in 1970 was classified into the same genotype as the ORFVs from Japanese serows. These results suggest that recent outbreaks in Japanese serows are caused by ORFVs genetically closely related to the viruses in earlier outbreaks and that these genetically stable ORFVs have circulated among Japanese serows over a long period of time.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Spatial and Temporal Genetic Homogeneity of Orf Viruses Infecting Japanese Serows (Capricornis crispus)

Inoshima

Ito

Ishiguro

2010

The Journal of Veterinary Medical Science

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“…PCR was evaluated as a fast, cheap and valid technique for virus detection. The most common genes used are B2L gene; a highly immunogenic envelope gene [19,26,28] and Orf F1L gene; an immunodominant gene [1,9,12,14,15,18,19,26,28].…”

Section: Introductionmentioning

confidence: 99%

Isolation and phylogenetic analysis of caprine Orf virus in Malaysia

et al. 2015

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Orf virus is a DNA virus that causes contiguous ecthyma in goat and sheep. Infection of animals with this virus cause high mortality in young animals resulting in huge economic losses. In this study, we investigated an outbreak of Orf in a goat farm in Malaysia. Samples were collected from infected animals and viral isolation was done using both LT and MDCK cell lines. Molecular detection was done by conventional PCR for specific primers; B2L and F1L genes and phylogenetic analysis was done on the sequence data obtained. Cytopathic effects (CPE) were observed in both cell lines after 3 days of inoculation and were 50 % by the sixth day. PCR showed positive bands for both B2L and F1L genes and phylogenetic analysis showed that the Malaysian strain had close homology to the Chinese and Indian Orf virus isolates. This study gives more insight into the existing Orf viral strains in Malaysia and their relationship with other strains globally.

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“…The introduction of new ORFV strains in Brazil seems to occur through the import of animals in order to improve herd genetics (Abrahão et al, 2009). …”

Section: Figure 10: Ultrathin Section Of the Crusts Fragments Observmentioning

confidence: 99%

Detection of Parapoxvirus in goats during contagious ecthyma outbreak in Ceará State, Brazil by transmission electron microscopy techniques

Catroxo¹,

Martins²,

Souza³

2017

IJOEAR

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Abstract-Contagious ecthyma or contagious pustular dermatitis, is a viral skin disease that occurs in sheep, goats and wild ruminants and is characterized by the formation of papules, nodules or vesicles that progress into thick crusts showing two morphological forms, a mulberry (M) form with a distinctive crisscross filament pattern derived from the superimposition of upper and lower virion surfaces and a capsular (C) form caused by stain penetration and distention of the virion core, measuring 300 x 180 nm was observed. Antigen antibody reaction was increased by the colloidal gold particles. In the ultrathin sections of crusts, we verified the presence of three types of intracytoplasmic inclusion bodies, type A or Bollinger inclusion bodies, outlined by membrane, presented in it

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Detection and phylogenetic analysis of Orf virus from sheep in Brazil: a case report

Cited by 42 publications

References 14 publications

Spatial and Temporal Genetic Homogeneity of Orf Viruses Infecting Japanese Serows (Capricornis crispus)

Spatial and Temporal Genetic Homogeneity of Orf Viruses Infecting Japanese Serows (Capricornis crispus)

Isolation and phylogenetic analysis of caprine Orf virus in Malaysia

Detection of Parapoxvirus in goats during contagious ecthyma outbreak in Ceará State, Brazil by transmission electron microscopy techniques

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