Detection and phylogenetic analysis of carp edema virus in common carp (Cyprinus Carpio) in Iran; 2020–2021

Kafi, Zahra Ziafati; Najafi, Hamideh; Alishahi, Mojtaba; Rahmati‐Holasoo, Hooman; Mouloki, Aidin; Ghalyanchilangeroudi, Arash

doi:10.1016/j.aquaculture.2022.738381

Cited by 7 publications

(4 citation statements)

References 37 publications

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“…The virus strains used here were CyHV-1, CyHV-3, and CEV, known as carp pox, koi herpesvirus, and carp edema virus, respectively. Positive samples were obtained from the veterinary medicine, university of Tehran, from previously con rmed positive specimens (Rahmati-Holasoo et al 2020; Ka et al 2022).…”

Section: Pathogen Collection and Dna Extractionmentioning

confidence: 99%

Rapid cyprinid herpesvirus 3 detection using loop-mediated isothermal amplification (LAMP) combined with gold nanoprobes and SYBR safe

Bavarsad,

Abed-Elmdoost,

Tabandeh

et al. 2025

Aquaculture

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Section: Pathogen Collection and Dna Extractionmentioning

confidence: 99%

Rapid cyprinid herpesvirus 3 detection using loop-mediated isothermal amplification (LAMP) combined with gold nanoprobes and SYBR safe

Bavarsad,

Abed-Elmdoost,

Tabandeh

et al. 2025

Aquaculture

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Section: Pathogen Collection and Dna Extractionmentioning

confidence: 99%

Rapid Cyprinid herpesvirus 3 detection using loop-mediated isothermal amplification (LAMP) combined with gold nanoprobes and SYBR safe

Bavarsad,

Abed-Elmdoost,

Tabandeh

et al. 2023

Preprint

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As an etiological agent of a notifiable and emerging disease, Cyprinid herpesvirus 3 (CyHV-3 or koi herpesvirus, KHV) is a highly risky factor that affects koi and common carp yield through increasing the mortality rates. In the current study, a highly rapid and sensitive technique was established to detect CyHV-3 with the help of one step loop-mediated isothermal amplification (LAMP) method with dual visualizing indicators (SYBR safe and gold nanoparticle probes; AuNP-probes). Six specific primers were used to amplify the thymidine kinase (TK) of CyHV-3 using a LAMP reaction of 45 min at 65°C along with detecting the products through visual inspection via hybridization at 65°C for 5 min with a functionalized thiol-AuNP probe. A closed-tube LAMP-SYBR safe assay was also developed under the same condition without AuNP probe. The amplification of KHV-LAMP products were evaluated by the naked eye, through fluorescent emission of LAMP-SYBR safe dye-complex and colorimetric aggregate of LAMP product in the presence of AuNP probe. Furthermore, quantitative measurement was applied to the LAMP-AuNPs assay, using the spectral shift assay. The detection limit was 1.25 fg DNA which can compete the latest techniques available. The proposed technique yielded negative outcomes with DNA template from other viruses such as CyHV-1 and CEV. The LAMP-SYBR safe and LAMP-AuNPs assays introduced in this article was very sensitive, fast, and reliable diagnostic tools for CyHV-3 detection.

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“…Fisheries and Aquatic Sciences of alloherpesviruses such as cyprinid herpesvirus-2 (CyHV-2), -3 (CyHV-3, commonly known as koi herpesvirus; KHV), and poxvirus including carp edema virus (CEV) (Ahmadivand et al, 2020;Badhusha et al, 2022;Kafi et al, 2022). CyHV-2 and KHV were first reported in the Japanese goldfish (Carassius auratus) in 1995 (Jung & Miyazaki, 1995), and common carp (Cyprinus carpio) in Israel and USA in 1998 (Perelberg et al, 2003), respectively.…”

Section: Introductionmentioning

confidence: 99%

Genetic characterization of alloherpesvirus (cyprinid herpesvirus-2 and koi herpesvirus) and poxvirus (carp edema virus) identified from domestic and imported cyprinids in Korea

Jeong¹,

Jeon²,

Choi³

et al. 2023

Fish Aquat Sci

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Cyprinids are popular species for aquaculture worldwide, with Asia being a significant contributor to their production. In Korea, common carp (Cyprinus carpio), koi carp (Cyprinus rubrofuscus), and goldfish (Carassius auratus) are cultivated domestically and imported for ornamental or human consumption purposes. Among the viruses that infect cyprinids, cyprinid herpesvirus-2 (CyHV-2), koi herpesvirus (KHV, also known as cyprinid herpesvirus-3), and carp edema virus (CEV) are of particular concern as they cause substantial economic losses to the aquaculture industry. In this study, we investigated these viruses in both of domestic and imported cyprinids. Our results revealed that CyHV-2 was only detected in imported goldfish from Thailand. To further investigate the genetic characteristics of them, the marker A region was analyzed. Despite belonging to the same cluster with isolates from China, France, Poland, and Israel, CyHV-2 detected in this study showed distinct differences in their repetitive sequence sizes. Furthermore, two different forms of KHV/CEV coinfection were identified from domestic koi carp, both of which exhibited typical symptoms. Phylogenetic analysis showed that one KHV isolate (ScKc-2105-K) was of the Asian type and closely related to isolates from Japan, Indonesia, Belgium, Taiwan, and China. Two CEV isolates (ScKc-2105-CE and GhKc-2207-CE) belonged to the IIa type and showed high similarity with isolates from the USA, France, and Korea. Notably, koi carp injected with cultured KHV (ScKc-2105-K) showed 78.0% cumulative mortality within 14 days post-injection (dpi). Our findings support the importance of regular surveillance of viral diseases in cyprinids.

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Detection and phylogenetic analysis of carp edema virus in common carp (Cyprinus Carpio) in Iran; 2020–2021

Cited by 7 publications

References 37 publications

Rapid cyprinid herpesvirus 3 detection using loop-mediated isothermal amplification (LAMP) combined with gold nanoprobes and SYBR safe

Rapid cyprinid herpesvirus 3 detection using loop-mediated isothermal amplification (LAMP) combined with gold nanoprobes and SYBR safe

Rapid Cyprinid herpesvirus 3 detection using loop-mediated isothermal amplification (LAMP) combined with gold nanoprobes and SYBR safe

Genetic characterization of alloherpesvirus (cyprinid herpesvirus-2 and koi herpesvirus) and poxvirus (carp edema virus) identified from domestic and imported cyprinids in Korea

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