Detection and partial sequence analysis ofHelicobacter pylori DNA in the bile samples

Lin, Tin-Tsan; Yeh, Chih-Ting; Wu, Cheng‐Shyong; Liaw, Yun–Fan

doi:10.1007/bf02209009

Cited by 77 publications

(55 citation statements)

References 24 publications

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“…Although Helicobacter pylori is recognized as a human pathogen associated with gastric lesions, recent studies have revealed convincingly the presence of several Helicobacter species in the hepatobiliary system. [7][8][9][10][11][12][13][14][15][16][17][18] A microorganism resembling H. pylori was firstly detected in resected gallbladder (GB) mucosa of patient with gallstone by Kawaguchi et al in 1996 7 and bile-resistant hepatic Helicobacter species such as H. bilis, H. pullorum, and F. rappini were extracted from GB mucosa and bile juice of patients with chronic cholecystitis, suggesting that these agents may be key elements in the development of various GB-related diseases, especially GB cancer. 9 The pres-ence of H. pylori DNA in gallstones was established by PCR in several reports.…”

Section: Introductionmentioning

confidence: 99%

“…9 The pres-ence of H. pylori DNA in gallstones was established by PCR in several reports. [12][13][14] Together with the discovery of H. pylori in bile juice, [16][17][18] this has led to the suggestion that Helicobacter species are etiological agents in gallstone formation. However, another study from Germany reported no association of Helicobacter species with gallstone formation suggesting possible ethical and regional differences.…”

Section: Introductionmentioning

confidence: 99%

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Identification of Helicobacter pylori in Gallstone, Bile, and Other Hepatobiliary Tissues of Patients with Cholecystitis

Lee

et al. 2010

Gut Liver

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Background/Aims: Bacterial infection is accepted as a precipitating factor in cholesterol gallstone formation, and recent studies have revealed the presence of Helicobacter species in the hepatobiliary system. We utilized the polymerase chain reaction (PCR) to establish the presence of bacterial DNA, including from Helicobacter species, in gallstones, bile juice, and gallbladder mucosa from patients with gallstones. Methods: At cholecystectomy, 58 gallstones, 48 bile samples, and 46 gallbladder mucosa specimens were obtained and subjected to nested PCR using specific 16S rRNA primers of H. pylori and other bacteria. Bacterial species were identified by DNA sequencing analysis. Bacterial 16S rRNA was detected in 25 out of 36 mixed-cholesterol gallstones, 1 out of 10 purecholesterol gallstones, and 9 out of 12 pigmented stones. Furthermore, 16S rDNA sequencing identified Escherichia coli, Pseudomonas, Citrobacter, Klebsiella, and Helicobacter species. Results: Helicobacter DNA was detected in 4 out of 58 gallstones, 6 out of 48 bile samples, and 5 out of 46 gallbladder specimens. Direct sequencing of Helicobacter amplicons confirmed strains of H. pylori in all four gallstones, five out of six bile samples, and three out of five gallbladder specimens. Almost all mixed-cholesterol gallstones appear to harbor bacterial DNA, predominantly E. coli. Conclusions: H. pylori was also found in the biliary system, suggesting that these bacteria are of etiological importance in gallstone formation. (Gut Liver 2010; 4:60-67)

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of Helicobacter pylori in Gallstone, Bile, and Other Hepatobiliary Tissues of Patients with Cholecystitis

Lee

et al. 2010

Gut Liver

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“…Whether H. pylori participates in the pathogenesis of biliary diseases is a question that has been addressed by several investigators. The DNA of H. pylori has been identified in the bile, liver, and biliary epithelium of patients with hepatobiliary diseases [1,2,3]. In regard to the biliary diseases, in one study, H. pylori specific ureB DNA was detected in the gallbladder tissue of a Japanese patient with gallstone and cholecystitis [4].…”

Section: Introductionmentioning

confidence: 99%

“…Similarly Chen et al [5] found H. pylori ureA and ureB DNA in 13.55% of the gallbladders of patients with cholecystitis. However, in a study evaluating the presence of H. pylori ureA DNA in the bile by nested PCR, Lin et al [1] observed a negative result in four patients with biliary diseases. Queiroz et al [6] were able to isolate H. pylori strain from the liver of a Brazilian patient with cirrhosis, which indicates that H. pylori may be viable in the human liver.…”

Section: Introductionmentioning

confidence: 99%

Helicobacter pylori DNA in gallbladder tissue of patients with cholelithiasis and cholecystitis

Yucebilgili

Mehmetoğlu

Güçin

et al. 2009

J Infect Dev Ctries

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Background: Earlier reports on the detection of H. pylori DNA in gallbladder tissue of patients with cholelithiasis and cholecystitis gave discordant results. Our aim was to detect the presence of H. pylori DNA and to determine whether a correlation can be established with the biliary diseases. Methodology: The study included a total of 68 patients 20 to 79 years of age. Fifty-three of the participants were females, of whom 33 had cholelithiasis and 20 did not. Out of the 15 male patients, 8 were had cholelithiasis and 7 did not. Gallbladder tissue specimens were taken from all patients undergoing cholecystectomy and processed immediately for histology, culture and PCR. Results: Histological examination revealed that 36 (68%) of 53 females and 9 (60%) of 15 males had cholecystitis. PCR results detected H. pylori DNA in 15 (22%) of 68 samples but no bacteria were isolated in culture. The presence of few bacteria, the geographic distribution of H. pylori strains, and the bile milieu inhibitory effect might be some of the reasons for growth failure. Conclusions: In this study although H. pylori DNA was detected by PCR in gallbladder tissue of patients with cholecystitis, a clinical correlation with biliary disease could not be established because several conditions were difficult to meet as discussed in the text.

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“…This knowledge led to investigations into a potential relationship between Helicobacter species and hepatobiliary diseases. The relationship between H. pylori and liver diseases has been assessed in humans, and several reports have demonstrated the prevalence of H. pylori in hepatobiliary diseases [20][21][22]. Previous study reported that H. pylori or related similar H. pylori could be detected in liver tissues from patients with cholestatic liver diseases [21].…”

Section: Discussionmentioning

confidence: 99%

Genetic characterization of Helicobacter pylori vacA and cagA genes in Thai gastro-duodenal and hepatobiliary patients

Boonyanugomol

Khuntikeo

Pugkhem

et al. 2017

J Infect Dev Ctries

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Introduction: H. pylori has been detected in patients with hepatobiliary diseases. It is currently unclear whether the H. pylori detected in hepatobiliary patients are genetically similar to those in gastro-duodenal patients. The aim of this study was to determine H. pylori vacA and cagA genotypes in Thai patients with gastro-duodenal and hepatobiliary diseases. Methodology: H. pylori DNA was extracted from samples from gastric biopsies of gastro-duodenal patients (n=100) and from bile samples of hepatobiliary patients (n=80). The vacA and cagA genotypes were performed via polymerase chain reaction (PCR) followed by DNA sequencing. Results: The vacA m1 was found in Thai hepatobiliary patients (90%) at a higher rate compared with gastro-duodenal patients (50%).The combined vacA s1a+c/m1 were mostly found in Thai gastro-duodenal and hepatobiliary patients. The cagA gene was detected in 94% of patients with gastro-duodenal diseases compared with 28.8% in those with hepatobiliary diseases (p<0.05). On the other hand, the Western type cagA was more prominent among hepatobiliary patients (100%) than gastro-duodenal patients (57.4%), and this type was grouped into same cluster with Thai gastro-duodenal patients via phylogenetic analysis. Conclusions: Based on vacA and cagA analysis, we conclude that infection with H. pylori in gastro-duodenal and hepatobiliary patients may be caused by the different H. pylori strains.

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Detection and partial sequence analysis ofHelicobacter pylori DNA in the bile samples

Cited by 77 publications

References 24 publications

Identification of Helicobacter pylori in Gallstone, Bile, and Other Hepatobiliary Tissues of Patients with Cholecystitis

Identification of Helicobacter pylori in Gallstone, Bile, and Other Hepatobiliary Tissues of Patients with Cholecystitis

Helicobacter pylori DNA in gallbladder tissue of patients with cholelithiasis and cholecystitis

Genetic characterization of Helicobacter pylori vacA and cagA genes in Thai gastro-duodenal and hepatobiliary patients

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