Detection and partial purification of a potent mitogenic factor for human thyroid follicular cells

Bond, Jane A.; Graham, Gerard J.; Freshney, M; Dawson, Timothy; Sawhney, N.; Williams, E. D.; Wynford-Thomas, David

doi:10.1016/0303-7207(92)90086-l

Cited by 6 publications

(1 citation statement)

References 13 publications

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“…SGHTL-189 cell cultures and bovine pitu¬ itary cell cultures produced simi¬ lar conditioned medium ir-bFGF concentrations, and higher concentrations than capillary endothelial cells . Thyroid-associated irbFGF could be derived from endothelial cells and fibroblasts (Kawakami et al 1991 ;Bond et al 1992) that often contaminate primary thyrocyte cultures (Ollis et al 1982). However, the data from transfected human thyroid epithelial cell cultures support thyro¬ cytes as the source of ir-bFGF.…”

Section: Effect Of Bfgf On Thyroid Cell Growthmentioning

confidence: 91%

The effect of basic fibroblast growth factor on the growth and function of human thyrocytes

Taylor

Millatt²,

Whitley³

et al. 1993

Journal of Endocrinology

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Basic fibroblast growth factor (bFGF) was quantitated in human primary thyrocyte cultures and thyroid cell lines produced by transfection with pSV3neo. Immunoreactive-bFGF (ir-bFGF) bound to heparin-Sepharose affinity columns eluted with 1.8-2.0 mol NaCl/l and had a molecular weight of approximately 17,000. Recombinant human bFGF in the presence of 5% serum increased the growth of transfected human thyrocytes but not the growth of primary human thyrocytes. Preincubation of cells with up to 100 micrograms bFGF/l potentiated TSH-stimulated cAMP release from the transfected cells but inhibited release from primary human thyroid cultures. bFGF may be an important modulator of thyroid cell function and growth.

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Section: Effect Of Bfgf On Thyroid Cell Growthmentioning

confidence: 91%