Detection and partial characterization of an isolate of Groundnut ringspot virus in Solanum sessiliflorum

Abstract

The cubiu (Solanum sessiliflorum) fruit, originating in the Amazon basin, is commonly used in that region for food, medicine, and cosmetics. In an experimental culture of cubiu, in order to evaluate its adaptation to conditions in the Northern region of the state of Rio de Janeiro, it was observed plants with mosaic symptoms. A cubiu plant was collected and analyzed to identify the etiological agent. After mechanical passage through a local lesion host, a host range test was performed. The virus induced chlorotic local lesions in Chenopodium quinoa, necrotic local lesions in Gomphrena globosa, mosaic in S. sessiliflorum, leaf and stem necrosis in tomato (Lycopersicon esculentum) 'Rutgers', mosaic and leaf distortion in Datura stramonium and Physalis floridana, and necrotic local lesions followed by systemic necrosis and plant death in four Nicotiana species. Electron microscopic observations of ultra thin sections from infected cubiu leaves showed the presence of spheroidal, membrane-bound particles typical of tospovirus species. Analysis of the nucleocapsid protein from concentrated virus particles indicated the presence of a 28 kDa protein. RT-PCR was performed after total RNA extraction from infected IPA-6 tomato leaves. A fragment of approximately 0,8 kbp corresponding to the N gene was amplified, cloned and sequenced. The N protein from the cubiu isolate was 95% homologous to the Groundnut ringspot virus (GRSV) protein, and no more than 85% homologous to those from Zucchini lethal chlorosis virus (ZLCV) and Chrysanthemun stem necrosis virus (CSNV), Tomato spotted wilt virus (TSWV), and Tomato chlorotic spot virus (TCSV). This is the first report of the occurrence of GRSV (or any other plant virus) in cubiu.

Plant Viruses Transmitted by Thrips

“…GRSV was also detected in stunted sweet pepper plants with foliar mosaics, foliar necrosis and ringspots in fruit in Sa˜o Paulo state (Colariccio et al, 2001b). In 2002, GRSV was identified in cubiu (Solanum sessiliflorum) with mosaic symptoms growing in the state of Rio de Janeiro (Boari et al, 2002).…”

Citrullus lanatus: un nuevo hospedante natural del Groundnut ringspot orthotospovirus en Argentina

“…Las modificaciones realizadas fueron: en la primera extracción, la mezcla cloroformo:alcohol isoamílico se agitó vigorosamente durante 10 min hasta obtener una emulsión. En la segunda extracción con cloroformo:alcohol isoamílico se mezcló la muestra durante 5 min vigorosamente hasta obtener una emulsión y luego del agregado de ClLi se incubó la muestra a -20º C. Se usaron cebadores específicos para GRSV reportados por Boari et al (2002). Se purificaron los productos de PCR de dos muestras con el kit Zymo (DNA clean & concentrator, Zymo Research Corp., Irvine, California, Estados Unidos) y fueron enviados para secuenciar a la Unidad de Genómica del Instituto de Biotecnología -INTA (Argentina).…”

A global invasion by the thrip, Frankliniella occidentalis: Current virus vector status and its management

“…It was subsequently reported from Argentina, the USA, the Caribbean basin and Ghana from a relatively narrow host range compared to TSWV (Webster et al ., ; Webster et al ., ; Camelo‐Garca et al ., ; Spadotti et al ., ; Leão et al ., ; Webster et al ., ; Appiah et al ., ). In Brazil, GRSV was identified from several hosts including sweet peppers, coriander, cocona, cucumbers, cubiu, peanuts and watermelons (Lima et al ., ; Boari et al ., ; Camelo‐Garca et al ., ; Spadotti et al ., ; Leão et al ., ). In North America, GRSV was initially reported from tomatoes in south Florida in 2009, subsequently from peppers, tomatilloes and eggplants in peninsular Florida, and later in South Carolina and New York (Webster et al ., , , ).…”