Detection and molecular identification of Blastocystis isolates from humans and cattle in northern Egypt

Abdo, Sarah; El-Adawy, Hosny; Farag, H.A.; El-Taweel, Hend A; Elhadad, Heba; El-Badry, Ayman A.

doi:10.1007/s12639-021-01354-5

Cited by 14 publications

(10 citation statements)

References 40 publications

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“…Elsewhere, strong molecular evidence has confirmed zoonotic transmission between animals and their human caregivers 37,38 . Notably, humans in direct contact with animals were found to have the same Blastocystis STs in two studies conducted in Northern and Eastern Egypt (ST1, ST2, ST3, and ST4) 37,39 . Additionally, poultry can harbor the human-transmissible Blastocystis ST6 and ST7 40 , although this has only been reported in a recent Egyptian study of colorectal cancer patients 41 .…”

Section: Discussionmentioning

confidence: 82%

“…Other subtypes (ST1, ST2, ST4 and ST7) have been detected in the Egyptian community, with varying frequencies depending on the sample size and testing technique used. ST1 and ST2 have been identified as relevant STs in a smaller number www.nature.com/scientificreports/ of Egyptian studies 28,39 . Notably, STs and their relative frequencies appear to vary significantly within a single country (Table 6).…”

Section: Discussionmentioning

confidence: 99%

“…Several Egyptian studies have subtyped and sequenced Blastocystis sp. 39 , 41 , 50 , with five STs (ST1, ST2, ST3, ST4 and ST7) identified at varying frequencies in distinct Egyptian groups using PCR sequenced-tagged sites, PCR restriction fragment length polymorphisms, and PCR sequencing (barcoding) (Table 6 ). The high prevalence of Blastocystis sp.…”

Section: Discussionmentioning

confidence: 99%

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Subtypes and phylogenetic analysis of Blastocystis sp. isolates from West Ismailia, Egypt

Ahmed

El-Mahallawy

Mohamed

et al. 2022

Sci Rep

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In Egypt, Blastocystis sp. is not yet on the diagnostic list of parasitology reports, and information about its subtypes (STs) is scarce. This study investigated its prevalence and its STs/alleles, performed phylogenetic analysis, and considered the distribution of risk factors associated with Blastocystis sp. infections in West Ismailia, Ismailia governorate. Sociodemographic data, exposure factors, and previous parasitic infection status were recorded for symptomatic and asymptomatic individuals. Microscopy, polymerase chain reaction, sequencing, and phylogenetic analysis for Blastocystis sp. isolated from fecal samples were performed. Eighty Blastocystis sp.-infected individuals (15.3%) were examined. The age of the individuals ranged between 0.60 and 85.0 (mean 17.10 ± 15.70), the male/female ratio was 33/47, and the asymptomatic/symptomatic ratio was 55/25. The findings demonstrate clear evidence of direct contact with animals, poor water quality, and previous parasitic infections. Eleven samples yielded three Blastocystis STs (ST1: allele 4, ST2: alleles 9 and 12, and ST3: allele 34), with ST3 (45.5%) representing the most common subtype. Phylogenetic analysis with a robust bootstrap revealed three distinct clades for isolates of each subtype. This study updates the epidemiological knowledge of the distribution of Blastocystis sp. STs in Egypt and expands the current understanding of the prevalence, risk factor frequencies, and genetic diversity of this protist in the studied area.

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Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Subtypes and phylogenetic analysis of Blastocystis sp. isolates from West Ismailia, Egypt

Ahmed

El-Mahallawy

Mohamed

et al. 2022

Sci Rep

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“…Blastocystis sp. and trichomonad species are newly emerging in both animals and human Abdo et al 2021). A previous study in in China reported that the prevalence of Blastocystis sp.…”

Section: Declarations Con Ict Of Interestmentioning

confidence: 99%

“…The reporting of P. hominis and Blastocystis sp. isolated from different animal species and their close phylogenetic relationship to human isolates indicates that both parasites may be zoonotically transmitted between humans and other animal hosts Abdo et al 2021).…”

Section: Declarations Con Ict Of Interestmentioning

confidence: 99%

Pentatrichomonas hominis and other intestinal parasites in school-aged children: Clinical and coproscopic survey

Abdo

Ghallab

Elhawary

et al. 2022

Preprint

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Pentatrichomonas hominis (P.hominis) is a large intestinal flagellated protozoan infecting humans. Little is known about the epidemiology of P.hominis in Egypt, its association with gastrointestinal symptoms and the co-infection with other parasites. Demographic and clinical data were collected from 180 school-aged children. Parasitological examination of fecal samples was done using direct wet mount, formalin ethyl-acetate, Kato-Katz and cultivation on Jones' medium to detect P.hominis and associated parasitic infection. The diagnosis of P.hominis was confirmed using Giemsa stain and scanning electron microscopy. The prevalence of P. hominis was 13.8% (25 out of 180 children). The prevalence of parasitic co-infection was significantly higher in P.hominis infected (84%, 21 participants) than in non-infected children (56%, 87 participants).The presence of abdominal pain and diarrhea in P.hominis infected children was higher than in non-infected children (84% and 32% vs. 76% and 18%), respectively. The difference was not statistically significant. This is the first report of P.hominis in Egypt. The significant association between P.hominis and other intestinal parasites may highlight the pathogenicity of P.hominis. Further studies are needed to understand the epidemiology and pathogenicity of P.hominis.

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Identification and genetic characterization of Blastocystis subtypes in Père David's deer (Elaphurus davidianus) from Shishou, China

Yang

et al. 2022

Vet Res Commun

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Detection and molecular identification of Blastocystis isolates from humans and cattle in northern Egypt

Cited by 14 publications

References 40 publications

Subtypes and phylogenetic analysis of Blastocystis sp. isolates from West Ismailia, Egypt

Subtypes and phylogenetic analysis of Blastocystis sp. isolates from West Ismailia, Egypt

Pentatrichomonas hominis and other intestinal parasites in school-aged children: Clinical and coproscopic survey

Identification and genetic characterization of Blastocystis subtypes in Père David's deer (Elaphurus davidianus) from Shishou, China

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