Detection and molecular characterization of Torque Teno Virus (TTV) in Uruguay

Cancela, Florencia; Ramos, Natália; Mirazo, Santiago; Mainardi, Victoria; Gerona, Solange

doi:10.1016/j.meegid.2016.08.007

Cited by 13 publications

(12 citation statements)

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“…It has been noted in several studies that restoration of the immune response may cause a reduction in TTV viral load/positivity detected in patient sera [ 27 , 48 , 49 ]. Overall, TTV infection was detected in 79.3% of patients, in agreement with previously reported data [ 14 , 41 , 50 , 51 , 52 ], and no correlation was observed between TTV positivity and achievement of SVR since TTV DNA prevalence was similar between HIV/HCV coinfected groups B and C and HCV monoinfected groups D and E ( Table 3 ). Analysis of gender relationships with TTV was non-conclusive since the majority of patients were males (88%).…”

Section: Discussionsupporting

confidence: 91%

“…Eluted nucleic acids (10 μL) were used for TTV-DNA detection by semi-nested PCR using primers designed against the untranslated genome B (UTRB) region, as previously described [ 41 ]. Primers were designed using the TTV TA278 isolate genome fragment spanning nucleotides 3362–3739 (Genbank accession number AB008394).…”

Section: Methodsmentioning

confidence: 99%

“… Phylogenetic analysis of TTV UTR B (3′UTR) region from patient-derived samples. Thirty-three patient-derived nucleotide sequences and representative TTV genogroup sequences, chosen following the original publications [ 8 , 14 , 41 , 45 , 46 ] and ICVT [ 47 ] were used for the construction of a Maximum-likelihood method tree that was visualized with Fig Tree (Available online: (accessed on 22 December 2020)). Bootstrap values > 80% are indicated on respective branches.…”

Section: Figurementioning

confidence: 99%

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Clinical Relevance of Torque Teno Virus (TTV) in HIV/HCV Coinfected and HCV Monoinfected Patients Treated with Direct-Acting Antiviral Therapy

Lapa

Porto

Minosse

et al. 2021

JCM

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Torque Teno virus (TTV) is a ubiquitous virus that causes chronic infection in humans with unknown clinical consequences. Here, we investigated the influence of TTV infection on HCV direct-acting antiviral (DAA) efficacy in HIV/HCV coinfected and HCV monoinfected patients as controls. Of 92 study patients, 79.3% were TTV DNA positive; untreated patients exhibited a significantly higher proportion of TTV DNA-positivity vs. sustained virological response (SVR) patients (100.0% vs. 65.2%, p < 0.001), while TTV positivity was not significant in DAA failure patients vs. SVR patients despite HIV/HCV coinfection. TTV DNA viral load was higher among HCV monoinfected patients vs. HIV/HCV coinfected, although marginally significant (p = 0.074) and no significant viral load difference was detected between DAA failures and SVR patients, while untreated vs. SVR patients had a significantly higher viral load (19,884, IQR 5977–333,534, vs. 469, IQR 10–4124, p = 0.004). Alpha-genogroup 3 TTV was the most prevalent genetic group, and no specific strain or genogroup was observed in relapser patients. Among HIV/HCV patients with HCV RNA detectable at end of treatment (EOT), TTV DNA was detected in 9/17 treatment responder patients and 3/5 relapser patients, thus, TTV infection does not appear to influence the control HCV viremia after EOT. Levels of IL-6 IL-4, and CD14 were not significantly different between TTV PCR-positive and -negative patients. These results suggest no association between TTV DNA positivity or viral load and HCV DAA failure whether patients were HIV/HCV coinfected or HCV monoinfected.

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Section: Discussionsupporting

confidence: 91%

Section: Methodsmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

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Clinical Relevance of Torque Teno Virus (TTV) in HIV/HCV Coinfected and HCV Monoinfected Patients Treated with Direct-Acting Antiviral Therapy

Lapa

Porto

Minosse

et al. 2021

JCM

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“…A blank containing nuclease-free water instead of DNA was included in each run of PCR. The thermal cycling conditions were set to as those reported by Cancela [27]. PCR products (110 bp) were detected in 3% agarose gel and were purified for DNA sequencing using Monarch PCR and DNA Cleanup Kit (New England Biolabs, Ipswich, MA, USA), as recommended by the manufacturer.…”

Section: Ttv Detection By Nested-utr-pcrmentioning

confidence: 99%

The Molecular Epidemiology and Phylogeny of Torque Teno Virus (TTV) in Jordan

Sarairah

Bdour

Gharaibeh

2020

Viruses

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Torque teno virus (TTV) is the most common component of the human blood virobiota. Little is known, however, about the prevalence of TTV in humans and the most common farm domesticates in Jordan, or the history and modality of TTV transmission across species lines. We therefore tested sera from 396 Jordanians and 171 farm animals for the presence of TTV DNA using nested 5′-UTR-PCR. We then performed phylogenetic, ordination and evolutionary diversity analyses on detected DNA sequences. We detected a very high prevalence of TTV in Jordanians (~96%); much higher than in farm animal domesticates (~29% pooled over species). TTV prevalence in the human participants is not associated with geography, demography or physical attributes. Phylogenetic, ordination and evolutionary diversity analyses indicated that TTV is transmitted readily between humans across the geography of the country and between various species of animal domesticates. However, the majority of animal TTV isolates seem to derive from a single human-to-animal transmission event in the past, and current human-animal transmission in either direction is relatively rare. In conclusion, animal TTV in Jordan is historically derived from human variants; however, ongoing human-animal TTV exchange is minimal and zoonotic infection seems to be of limited importance.

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“…В Венгрии, на Ближнем Востоке доминирует третий (65,5 %), в меньшей степени распространены 5-й (24 %), 2-й (5,8 %) и 1-й (4,7 %) генотипы [7,16,[18][19][20][21][22][23][24][25]. Для Южной Америки характерны в порядке убывания 1-, 2-и 3-й генотипы [26]. Генотипы 1 и 2 TTV являются доминирующими в Корее [27].…”

Section: генетическая организация Torque Teno Virusunclassified

Current Views of Torque Teno Virus (TTV) in Liver Diseases

Маев

Карлович

Burmistrov

et al. 2020

Rossijskij žurnal gastroènterologii, gepatologii, koloproktolog

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Цель обзора. Обзор посвящен анализу данных литературы по torque teno virus (вирус TT, TTV), открытому в конце XX столетия. Основные положения: TTV часто обнаруживают у пациентов с различными вирусными гепатитами, гепатитами без явного вирусного агента, а также у здорового населения. Имеются отдельные данные о биохимических и гистологических изменениях в ткани печени и эпителии желчных протоков при моноинфицировании TTV, подтверждающие возможность прохождения вирусом репликативного цикла в гепатоцитах. Наряду с этим при проведении гибридизации в клетках, пораженных вирусом ТТ, не было отмечено существенных цитопатических (повреждающих клетки) эффектов, характерных для патогенных гепатотропных вирусов. По мере изучения TTV происходила эволюция взглядов на роль вируса для человека. Первые представления о гепатотропности вируса постепенно изменялись по мере получения новых данных о распространенности вируса и его коинфекции с другими вирусами, в том числе вирусами известных гепатитов. Данные о политропизме TTV опровергают возможность его отнесения исключительно к вирусам гепатита. Высокая распространенность вируса ТТ в человеческой популяции указывает на его персистенцию в организме в составе вирома и свидетельствует в пользу его непатогенности. Предлагается использовать уровень ДНК TTV в крови пациентов перед трансплантацией органов в качестве эндогенного маркера иммунного статуса организма. Выводы: Высокая распространенность вируса ТТ в человеческой популяции указывает на его персистенцию в организме в составе вирома. Редкое выявление повреждающего действия TTV на клетки печени и эпителио циты желчных протоков может косвенно свидетельствовать о его условно-патогенных свойствах. Ключевые слова: torque teno virus, виром, гепатит, вирусы гепатита, иммуносупрессия, трансплантация Конфликт интересов: Авторы заявляют об отсутствии конфликтов интересов.

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Detection and molecular characterization of Torque Teno Virus (TTV) in Uruguay

Cited by 13 publications

References 83 publications

Clinical Relevance of Torque Teno Virus (TTV) in HIV/HCV Coinfected and HCV Monoinfected Patients Treated with Direct-Acting Antiviral Therapy

Clinical Relevance of Torque Teno Virus (TTV) in HIV/HCV Coinfected and HCV Monoinfected Patients Treated with Direct-Acting Antiviral Therapy

The Molecular Epidemiology and Phylogeny of Torque Teno Virus (TTV) in Jordan

Current Views of Torque Teno Virus (TTV) in Liver Diseases

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