Detection and molecular characterisation of noroviruses and sapoviruses in asymptomatic swine and cattle in Slovenian farms

Mijovski, Janet Zimšek; Poljšak-Prijatelj, Mateja; Steyer, Andrej; Barlič–Maganja, Darja; Koren, Simon

doi:10.1016/j.meegid.2009.11.010

Cited by 44 publications

(49 citation statements)

References 41 publications

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“…This is in agreement with other studies which found various genotype distributions, usually with GII.18 or GII.11 as the predominant strains (5,15,19). The GII.11-like NoV-positive samples showed no amplicons using primers G2SKF and G2SKR for capsid sequencing; therefore, it is possible that some of these strains are misclassified because only a short fragment from the polymerase region was sequenced using primers p290 and p110.…”

Section: Discussionsupporting

confidence: 91%

“…Sapoviruses (SaVs), which belong to Sapovirus genus of Caliciviridae, are also important enteric pathogens affecting both humans and swine (3,4). Subclinical infections with NoVs and SaVs are common and can occur in humans as well as in pigs (1,5,6). Noroviruses have mainly been found in finisher pigs, while SaVs have been detected in all age groups of pigs (7,8).…”

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confidence: 99%

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Prevalence of Porcine Noroviruses, Molecular Characterization of Emerging Porcine Sapoviruses from Finisher Swine in the United States, and Unified Classification Scheme for Sapoviruses

et al. 2013

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Noroviruses (NoVs) and sapoviruses (SaVs) are important human pathogens. Although the involvement of porcine NoVs in disease in pigs is unclear, they are genetically and antigenically closely related to human NoVs. Human NoV-like strains have been detected in pigs, raising public health concerns of potential interspecies transmission. Porcine SaVs are highly diverse and emerging in swine populations. Recently, at least three new genogroups of porcine SaVs have been proposed. In this study, we tested 413 pooled fecal samples collected from apparently healthy finisher pigs in North Carolina swine farms during 2009. Reverse transcription (RT)-PCR coupled hybridization assays were performed to detect known porcine NoVs. The overall prevalence of porcine NoVs determined was 18.9% based on this method. Samples were then tested by RT-PCR targeting the 5= end of the capsid region for genogroup II (GII) NoVs, a group which includes human NoVs, followed by sequence analysis. All NoVs identified belonged to typical porcine NoV genotypes, and no human NoV-like strains were detected in specimens from these pigs. Porcine NoV-negative samples (n ‫؍‬ 335) were subsequently screened using universal calicivirus primers, and 17 SaV strains were confirmed by sequencing. Based on the partial RNA-dependent RNA polymerase (RdRp) region, they clustered with GIII, GVII, and GVIII and with currently unclassified SaVs. According to analysis of the complete capsid sequences, 7 representative strains clustered with GVII, GVIII, and GIX? SaVs. We tentatively classified SaVs into 14 genogroups based on the complete capsid protein VP1. In summary, porcine NoVs and highly divergent SaVs were present in North Carolina finisher pigs.

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Section: Discussionsupporting

confidence: 91%

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confidence: 99%

Prevalence of Porcine Noroviruses, Molecular Characterization of Emerging Porcine Sapoviruses from Finisher Swine in the United States, and Unified Classification Scheme for Sapoviruses

et al. 2013

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“…Molecular diagnostics performed in different countries have established a worldwide distribution of NoV in pigproduction systems (Wolf et al 2009;Keum et al 2009;L'Homme et al 2009;Mijovski et al 2010;Cunha et al 2010a). In general, the reported rates of PoNoV detection were low, ranging from 0 to 2 % (Martínez et al 2006;Keum et al 2009;Mijovski et al 2010) to 20 % (Wang et al 2006b).…”

Section: Resultsmentioning

confidence: 99%

“…NoVs have been detected in human and in different animal species, such as bovine, swine, ovine, canine and feline (Martella et al 2007(Martella et al , 2008Patel et al 2009;Wolf et al 2009;Mijovski et al 2010;Pinto et al 2012). Human and animal NoVs are non-enveloped and have a positive-sense, single-stranded RNA genome of approximately 7.5 kb (Green 2007).…”

Section: Introductionmentioning

confidence: 99%

High frequency of porcine norovirus infection in finisher units of Brazilian pig-production systems

Silva

Alfieri

Barry

et al. 2014

Trop Anim Health Prod

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Norovirus (NoV) is a member of the Caliciviridae family and is considered an emerging human enteric pathogen. NoVs are detected in farm animals such as cattle, sheep and pigs. Porcine NoV (PoNoV) is widespread worldwide, but frequency of infection is often low. This study aimed to investigate the natural PoNoV infection from adult animals of an important Brazilian pig-production region. Faecal samples (n = 112) of asymptomatic pigs aged 9 to 24 weeks old were collected from 16 grower-to-finish herds located in Paraná state, Brazilian Southern region, and evaluated for PoNoV presence. A reverse transcription-polymerase chain reaction (RT-PCR) assay was performed using specific primers that target a conserved region of the virus capsid gene (VP1). PoNoV was detected in 58 (51.8%) of the 112 faecal samples and in 14 (87.5%) of the 16 herds evaluated. Six of the obtained amplicons were submitted to phylogenetic genotyping analysis. The higher nucleotide (86.5-97.4%) and amino acid (100%) similarities of the sequences in this study were with the representative strains of the porcine NoV genogroup II genotype 11 (PoNoV GII-11). These results reveal that PoNoV infection is endemic in one of the most important pork production areas of Brazil and that the PoNoV GII-11 is prevalent in this region.

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“…in asymptomatic swine in Slovenia [25] and 0% in US pigs [26]. The difference in the occurrence rate for detecting Norovirus in swine may be attributed to the geographical sites, ages of swine and variation in detection methods being used [27].…”

Section: Discussionmentioning

confidence: 99%

Occurrence of Norovirus in pig faecal samples in the Eastern Cape, South Africa

Taku

Iweriebor

Nwodo

et al. 2017

APJTD

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Detection and molecular characterisation of noroviruses and sapoviruses in asymptomatic swine and cattle in Slovenian farms

Cited by 44 publications

References 41 publications

Prevalence of Porcine Noroviruses, Molecular Characterization of Emerging Porcine Sapoviruses from Finisher Swine in the United States, and Unified Classification Scheme for Sapoviruses

Prevalence of Porcine Noroviruses, Molecular Characterization of Emerging Porcine Sapoviruses from Finisher Swine in the United States, and Unified Classification Scheme for Sapoviruses

High frequency of porcine norovirus infection in finisher units of Brazilian pig-production systems

Occurrence of Norovirus in pig faecal samples in the Eastern Cape, South Africa

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