Detection and Localization of Two Constitutive NOS Isoforms in Bull Spermatozoa

Meiser, Heribert; Schulz, Rüdiger

doi:10.1111/j.1439-0264.2003.00459.x

Cited by 37 publications

(34 citation statements)

References 23 publications

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“…NO has been demonstrated to play a role in a variety of functions in the human reproductive tract, including sperm motility (Lewis et al 1996), chemotaxis (Miraglia et al 2007), and sperm-zona pellucida binding ability (Sengoku et al 1998). NOS isoforms have been localized in the acrosome and tail of human, mouse, and bovine spermatozoa (Herrero et al 1996, Meiser & Schulz 2003, and low motility spermatozoa have been shown to exhibit aberrant patterns of NOS3 immunostaining (O'Bryan et al 1998). It has been reported that low concentrations of NO (25-100 nM sodium nitroprusside) enhance the motility of human spermatozoa (Hellstrom et al 1994, Zhang & Zheng 1996.…”

Section: Introductionmentioning

confidence: 99%

Nitric oxide stimulates human sperm motility via activation of the cyclic GMP/protein kinase G signaling pathway

Miraglia¹,

Angelis²,

Gazzano³

et al. 2011

Reproduction

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Nitric oxide (NO), a modulator of several physiological processes, is involved in different human sperm functions. We have investigated whether NO may stimulate the motility of human spermatozoa via activation of the soluble guanylate cyclase (sGC)/cGMP pathway. Sperm samples obtained by masturbation from 70 normozoospermic patients were processed by the swim-up technique. The kinetic parameters of the motile sperm-rich fractions were assessed by computer-assisted sperm analysis. After a 30-90 min incubation, the NO donor S-nitrosoglutathione (GSNO) exerted a significant enhancing effect on progressive motility (77, 78, and 78% vs 66, 65, and 62% of the control at the corresponding time), straight linear velocity (44, 49, and 48 mm/s vs 34, 35, and 35.5 mm/s), curvilinear velocity (81, 83, and 84 mm/s vs 68 mm/s), and average path velocity (52, 57, and 54 mm/s vs 40, 42, and 42 mm/s) at 5 mM but not at lower concentrations, and in parallel increased the synthesis of cGMP. A similar effect was obtained with the NO donor spermine NONOate after 30 and 60 min. The GSNO-induced effects on sperm motility were abolished by 1H-[1,2,4]oxadiazolo-[4,3-a]quinoxalin-1-one (a specific sGC inhibitor) and mimicked by 8-bromo-cGMP (8-Br-cGMP; a cell-permeating cGMP analog); the treatment with Rp-8-Br-cGMPS (an inhibitor of cGMP-dependent protein kinases) prevented both the GSNO-and the 8-Br-cGMP-induced responses. On the contrary, we did not observe any effect of the cGMP/PRKG1 (PKG) pathway modulators on the onset of hyperactivated sperm motility. Our results suggest that NO stimulates human sperm motility via the activation of sGC, the subsequent synthesis of cGMP, and the activation of cGMP-dependent protein kinases.

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Section: Introductionmentioning

confidence: 99%

Nitric oxide stimulates human sperm motility via activation of the cyclic GMP/protein kinase G signaling pathway

Miraglia¹,

Angelis²,

Gazzano³

et al. 2011

Reproduction

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“…NO is synthesized in mature sperm by the NO synthase (NOS), which exists as three isoforms -neural NOS (nNOS), endothelial NOS (eNOS), and inducible NOS -and localize in sperm head and midpiece (Meiser & Schulz 2003). The constitutively expressed nNOS and eNOS isoforms are Ca 2C /calmodulin (CaM) dependent (Herrero & Gagnon 2001).…”

Section: Introductionmentioning

confidence: 99%

SPINK3 modulates mouse sperm physiology through the reduction of nitric oxide level independently of its trypsin inhibitory activity

Zalazar

Lancellotti²,

Clementi

et al. 2012

Reproduction

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Serine protease inhibitor Kazal-type (SPINK3)/P12/PSTI-II is a small secretory protein from mouse seminal vesicle which contains a KAZAL domain and shows calcium (Ca 2C )-transport inhibitory (caltrin) activity. This molecule was obtained as a recombinant protein and its effect on capacitated sperm cells was examined. SPINK3 inhibited trypsin activity in vitro while the fusion protein GST-SPINK3 had no effect on this enzyme activity. The inactive GST-SPINK3 significantly reduced the percentage of spermatozoa positively stained for nitric oxide (NO) with the specific probe DAF-FM DA and NO concentration measured by Griess method in capacitated mouse sperm; the same effect was observed when sperm were capacitated under low Ca 2C concentration, using either intracellular (BAPTA-AM) or extracellular Ca 2C (EDTA) chelators. The percentage of sperm showing spontaneous and progesterone-induced acrosomal reaction was significantly lower in the presence of GST-SPINK3 compared to untreated capacitated spermatozoa. Interestingly, this decrease was overcome by the exogenous addition of the NO donors, sodium nitroprusside (SNP), and S-nitrosoglutathione (GSNO). Phosphorylation of sperm proteins in tyrosine residues was partially affected by GST-SPINK3, however, only GSNO was able to reverse this effect. Sperm progressive motility was not significantly diminished by GST-SPINK3 or BAPTA-AM but enhanced by the addition of SNP. This is the first report that demonstrates that SPINK3 modulates sperm physiology through a downstream reduction of endogenous NO concentration and independently of SPINK3 trypsin inhibitory activity.

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“…Thus nitric oxide appears to be crucial for the processes driving successful fertilization (reviewed in [46,47]). NOS isoforms were found in sperm of different mammal species such as mice [48], bulls [49], boars [50], and humans [51]. The abovementioned and well-known duality of NO's effects, depending upon concentrations, was also described in spermatozoa.…”

Section: No Roles In Spermatozoamentioning

confidence: 95%

From Nitric Oxide Toward S-Nitrosylation: Expanding Roles in Gametes and Embryos

Ješeta¹,

Sedmı́ková²,

Bodart³

2017

Nitric Oxide Synthase - Simple Enzyme-Complex Roles

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Nitric oxide (NO) is a gasotransmitter involved in various aspects of reproduction. The observational data from different species, such as sea urchin, ascidians, amphibians, rodents, porcine, bovine, and human, suggest that NO might have a significant role in reproduction through several mechanisms. This proposed role might appear preserved through evolution; however, the effects of NO also depend on the species or stages considered. There has been debate over the physiological relevance of NO, though the benefits of its use in assisted reproduction are now widely recognized. Over the past years, S-nitrosylation has provided a new angle to decipher the mechanisms through which NO exerts its actions. This chapter summarizes, in a nonexhaustive manner, research that explores the role of NO in gametes and embryos.

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Detection and Localization of Two Constitutive NOS Isoforms in Bull Spermatozoa

Cited by 37 publications

References 23 publications

Nitric oxide stimulates human sperm motility via activation of the cyclic GMP/protein kinase G signaling pathway

Nitric oxide stimulates human sperm motility via activation of the cyclic GMP/protein kinase G signaling pathway

SPINK3 modulates mouse sperm physiology through the reduction of nitric oxide level independently of its trypsin inhibitory activity

From Nitric Oxide Toward S-Nitrosylation: Expanding Roles in Gametes and Embryos

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