Detection and isolation of small ruminant lentivirus in the amniotic fluid of goats

Araújo, Juscilânia Furtado; Pinheiro, Alyson Andrade; Pinheiro, R. R.; Peixoto, Renato Mesquita; Sousa, Ana Lídia Madeira de; Azevedo, Dalva Alana Aragão de; Lima, Ana Milena César; Nobre, Juliana A.; Amaral, Gabriel Paula; Brandão, Iane Sousa; Teixeira, Maria Fátima da Silva

doi:10.1016/j.cimid.2021.101693

Cited by 3 publications

(1 citation statement)

References 34 publications

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“…Hence, the data obtained in this study performed with co-culture of SC of colostrum and SC of milk and OTE cells after use of ethanolic extracts of A. indica and M. azedarach may reveal a possible antiviral activity against CLV. This fact was observed mainly in the organic fraction of ethyl acetate, with an action time of 90 min, with marked inhibition of syncytium formation and cell destruction, which are considered the main characteristic cytopathic effects of CVL elucidated even in previous research studies, where the adoption of a qualitative evaluation through the visualization of their occurrence levels in cultured cells is standard 19,[42][43][44][45][46] .…”

Section: Discussionmentioning

confidence: 95%

In vitro antiviral effect of ethanolic extracts from Azadirachta indica and Melia azedarach against goat lentivirus in colostrum and milk

Sousa

Pinheiro

Araújo³

et al. 2023

Sci Rep

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This study aimed to evaluate, in vitro, the use of leaf extracts of Azadirachta indica (A. indica) and Melia azedarach (M. azedarach) as antivirals against caprine lentivirus (CLV) in colostrum and milk of goat nannies. These were collected from eight individuals and infected with the standard strain of CLV. Samples were then subdivided into aliquots and treated with 150 µg/mL of crude extract, and with ethyl acetate and methanol fractions for 30, 60, and 90 min. Next, somatic cells from colostrum and milk were co-cultured with cells from the ovine third eyelid. After this step, viral titers of the supernatants collected from treatments with greater efficacy in co-culture were assessed. The organic ethyl acetate fractions of both plants at 90 min possibly inhibited the viral activity of CLV by up to a thousandfold in colostrum. In milk, this inhibition was up to 800 times for the respective Meliaceae. In conclusion, the ethanolic fraction of ethyl acetate from both plants demonstrated efficacy against CLV in samples from colostrum and milk when subjected to treatment, which was more effective in colostrum.

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Section: Discussionmentioning

confidence: 95%

In vitro antiviral effect of ethanolic extracts from Azadirachta indica and Melia azedarach against goat lentivirus in colostrum and milk

Sousa

Pinheiro

Araújo³

et al. 2023

Sci Rep

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In vivo evaluation of the antiretroviral activity of Melia azedarach against small ruminant lentiviruses in goat colostrum and milk

Souza,

Pinheiro,

Peixoto

et al. 2023

Braz J Microbiol

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Susceptibility of different TMEM154 genotypes in three Italian sheep breeds infected by different SRLV genotypes

Moretti

Sartore

Colitti

et al. 2022

Vet Res

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Small ruminant lentiviruses (SRLV) belong to the Retroviridae family and can cause various diseases. One of the most impacting diseases is visna-maedi, a complex disease characterized by long latencies and chronic progressive inflammatory events affecting the nervous system, lungs, mammary gland, and articular joints. A single nucleotide polymorphism (rs408593969, c.103G>A, missense mutation E35K) in the ovine transmembrane protein gene 154 (TMEM154) was identified as protective against small ruminant lentivirus infection in different herds worldwide. However, there is evidence in the scientific literature of a breed-specificity of this protective effect and, furthermore, there are still limited studies regarding the association between the animal genotype and the infecting virus genotype. Thus, the aim of this study was to further investigate the association between the animal genotype for the suggested protective mutation and the infecting virus genotype, in three different sheep breeds reared in northern Italy. The results obtained only partially confirmed the data available in the literature, as the protective effect was confirmed only for SRLV genotype A clusters, while other genotypes (namely B and E) infected AA and GA animals. Further studies with an experimental infection of specific virus genotypes in hosts with specific genotypes are required to confirm the larger number of cases the results obtained in this study.

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Detection and isolation of small ruminant lentivirus in the amniotic fluid of goats

Cited by 3 publications

References 34 publications

In vitro antiviral effect of ethanolic extracts from Azadirachta indica and Melia azedarach against goat lentivirus in colostrum and milk

In vitro antiviral effect of ethanolic extracts from Azadirachta indica and Melia azedarach against goat lentivirus in colostrum and milk

In vivo evaluation of the antiretroviral activity of Melia azedarach against small ruminant lentiviruses in goat colostrum and milk

Susceptibility of different TMEM154 genotypes in three Italian sheep breeds infected by different SRLV genotypes

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