Detection and isolation of proctolin-like immunoreactivity in arachnids: Possible cardioregulatory role for proctolin in the orb-weaving spiders Argiope and Araneus

Groome, James R.; Townley, Mark A.; Tschaschell, Marianne de; Tillinghast, Edward K.

doi:10.1016/0022-1910(91)90013-p

Cited by 43 publications

(30 citation statements)

References 33 publications

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“…The specimens were dissected under a dissecting light microscope in a drop of spider Ringer's solution consisting of 160 mM NaCl, 7.5 mM KC1, 4 mM CaCl, 1 mM MgCl, 4 mM NaHCCh, and 20 mM glucose, pH 7.4 (Groome et al, 1991). For light microscopic observation, the tissues were fixed with buffered, neutral formalin solution, and were dehydrated using ethanol and embedded in Paraplast plus medium (Pofyscience) via xylene.…”

Section: Methodsmentioning

confidence: 99%

Fine structure of the glandular epithelium during secretory silk production in the black widow spiderlatrodectus mactans

Moon

Tillinghast

2002

Korean Journal of Biological Sciences

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Section: Methodsmentioning

confidence: 99%

Fine structure of the glandular epithelium during secretory silk production in the black widow spiderlatrodectus mactans

Moon

Tillinghast

2002

Korean Journal of Biological Sciences

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“…Female specimens were dissected under light microscope in a drop of spider Ringer's solution consisting of 160mM NaCI, 7.5 mM KCI, 4mM CaClg, 1 mM MgCl 2 , 4 mM NaCI, 7.5 mM glucose, pH 7.4 (Groome et al, 1991). The silk glands were gently removed, and then fixed in a mixture of 2% paraformaldehyde and 2.5% glutaraldehyde buffered with cacodylate buffer (Karnovsky, 1965).…”

Section: Methodsmentioning

confidence: 99%

Fine structural analysis of secretory silk production in the black widow spider,Latrodectus mactans

Moon¹,

Townley²,

Tillinghast³

1998

Korean Journal of Biological Sciences

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“…Paramount among these may be the existence of significant homology between many vertebrate and invertebrate peptides (49). Furthermore, some of the insect neuropeptides have shown activity in vertebrate (255,256) and non-insect, invertebrate systems (257,258).…”

Section: Discussionmentioning

confidence: 99%

“…The diapause hormone, isolated from 55,000 complexes of the subesophageal and first thoracic ganglion of day-1, B. mori pupae, was recently sequenced (257). It is a 24-residue peptide (Thr-Asp-Met-Lys-Asp-Glu-Ser-AspArg-Gly-Ala-His-Ser-Glu-Arg-Gly-Ala-Leu-Cys-Phe-Gly-Pro-Arg-Leu-NHU) containing the C-terminal pentapeptide sequence (Phe-X-Pro-Arg-Leu-NH^j necessary for activity (252) and homologous to the C-terminal sequences of the PBANs and the myotropic pyrokinins (see previous sections).…”

Section: Novel Insect Neurohormonesmentioning

confidence: 99%

Insect Neuropeptides

Kelly

Masler

Menn

1993

Natural and Engineered Pest Management Agents

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The physiological processes regulated by insect neuropeptides are extensive and include growth, development, molting, reproduction, diapause, behavior, color change, ion and water balance, and muscle contraction. Nearly 80 novel insect neuropeptides have been sequenced to date, 10 or so gene sequences have been determined, numerous analogs have been synthesized, and neuropeptide genes have been expressed in vector systems. Investigations into sites of synthesis and release, and tissue specificity and action, continue to reveal complexity in the (classically simple) insect neuroendocrine system. The rapidly increasing knowledge in this area suggests that useful prototypes for the design of selective pest control agents will emerge in the forseeable future.The field of insect neuroendocrinology has expanded rapidly, fostering numerous reviews within the past five years (1-21), including one book entirely devoted to insect neurohormones (22) and one to insect neuropeptides (23). These publications discuss the discovery, physiology, history and chemistry of insect neuropeptides and present potential applications to pest control. These applications may include: blocking neuropeptide synthesis through expression of antisense RNA or inhibition of specific processing enzymes; inactivation of circulating neuropeptides by binding with specific antibodies, antipeptides or by degradation with specific peptidases; disruption of target binding or signal transduction by peptidomimetics; and disruptive expression of natural or modified neuropeptides through baculoviruses or other expression vectors. As suspected in 1988 (4), the number of purified and sequenced insect neuropeptides has continued to increase, and many of the corresponding nucleotide sequences have followed in rapid succession. Nearly eighty novel amino acid sequences for insect neuropeptides have been identified, and novel nucleotide sequences approach ten. This review updates the recent progress in identification of insect neuropeptide structures and their genes and other areas relevant to the development of pest control agents, This chapter not subject to U.S.

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Detection and isolation of proctolin-like immunoreactivity in arachnids: Possible cardioregulatory role for proctolin in the orb-weaving spiders Argiope and Araneus

Cited by 43 publications

References 33 publications

Fine structure of the glandular epithelium during secretory silk production in the black widow spiderlatrodectus mactans

Fine structure of the glandular epithelium during secretory silk production in the black widow spiderlatrodectus mactans

Fine structural analysis of secretory silk production in the black widow spider,Latrodectus mactans

Insect Neuropeptides

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