Detection and interpretation of 8-oxodG and 8-oxoGua in urine, plasma and cerebrospinal fluid

Poulsen, Henrik E.; Nadal, Laia Llovera; Broedbaek, Kasper; Nielsen, Peter E.; Weimann, Allan

doi:10.1016/j.bbagen.2013.06.009

Cited by 144 publications

(118 citation statements)

References 82 publications

Supporting

Mentioning

112

Contrasting

Unclassified

Order By: Relevance

“…8‐Oxo‐2′‐dG is also clearly associated with kidney function. In most previous studies, the failure to adjust for renal clearance clearly undermines conclusions based on free 8‐oxo‐2′‐dG levels in the circulation or urine 21. Certainly, in our study, some of the association between plasma 8‐oxo‐2′‐dG levels and mortality was explained by the confounding effect of renal impairment.…”

Section: Discussioncontrasting

confidence: 65%

See 1 more Smart Citation

Relationship Between Plasma 8‐OH‐Deoxyguanosine and Cardiovascular Disease and Survival in Type 2 Diabetes Mellitus: Results From the ADVANCE Trial

Thomas

Woodward

et al. 2018

JAHA

View full text Add to dashboard Cite

Background8‐Oxo‐2′‐deoxyguanosine (8‐oxo‐2′‐dG) is a biomarker of oxidative DNA damage that is associated with cardiovascular disease and premature mortality in the general population. Although oxidative stress has a proven role in cardiovascular complications in diabetes mellitus, evidence for a relationship between plasma 8‐oxo‐2′‐dG and major cardiovascular outcomes in diabetes mellitus is weak.Methods and ResultsA case‐cohort study was performed in 3766 participants with prevalent diabetes mellitus in the ADVANCE (Action in Diabetes and Vascular Disease: Preterax and Diamicron Modified Release Controlled Evaluation) trial (http://ClinicalTrials.gov number NCT00145925). The hazard ratios for mortality and major acute cardiovascular events were derived using Cox regression models. During a median of 5 years of follow‐up, 695 (18.4%) participants in this enriched cohort died (including 354 deaths from cardiovascular disease). Individuals with higher levels of 8‐oxo‐2′‐dG were more likely to die. After adjusting for cardiovascular disease risk factors, the hazard ratio for a 1‐SD increase in plasma 8‐oxo‐2′‐dG was 1.10 (95% confidence interval, 1.01–1.20; P=0.03). This was driven by an independent association between plasma 8‐oxo‐2′‐dG and cardiovascular death (hazard ratio, 1.23; 95% confidence interval, 1.10–1.37 [P<0.001]). By contrast, no association was seen between 8‐oxo‐2′‐dG and noncardiovascular disease death (of which cancer was the major single cause). 8‐Oxo‐2′‐dG was also not significantly associated with either nonfatal myocardial infarction or nonfatal stroke.ConclusionsIn adults with type 2 diabetes mellitus, increased levels of 8‐oxo‐2′‐dG are independently associated with all‐cause mortality and cardiovascular mortality in adults with longstanding type 2 diabetes mellitus who participated in the ADVANCE trial, consistent with the role of oxidative damage in the development and progression of cardiovascular decompensation in diabetes mellitus.Clinical Trial Registration URL: http://www.clinicaltrials.gov. Unique identifier: NCT00145925.

show abstract

Section: Discussioncontrasting

confidence: 65%

“…Although widely considered a marker of oxidative DNA damage, 8‐oxo‐2′‐dG may also partly reflect the rate of its liberation via DNA repair mechanisms1, 21, 22 and oxidatively damaged 2′‐deoxyguanosine from the nucleotide pool. 8‐Oxo‐2′‐dG is also clearly associated with kidney function.…”

Section: Discussionmentioning

confidence: 99%

Relationship Between Plasma 8‐OH‐Deoxyguanosine and Cardiovascular Disease and Survival in Type 2 Diabetes Mellitus: Results From the ADVANCE Trial

Thomas

Woodward

et al. 2018

JAHA

View full text Add to dashboard Cite

show abstract

“…Hence, elevated levels of urinary 8-OHdG as a biomarker of oxidative stress (ROS) are observed in patients with type 1 and 2 DM as well as in cancer 22,23 .…”

Section: Resultsmentioning

confidence: 99%

Untitled

2017

IJPSR

View full text Add to dashboard Cite

Both diabetes mellitus and cancer are characterised by higher levels of oxidative stress and production of free radicals, which further complicate the control and outcomes of these diseases. The aim of this study was to assess the level of urinary 8-Hydroxydeoxyguanosine in diabetic cancer patients as a biomarker of cellular oxidative stress for both disease progression and chemotherapy administration. A controlled prospective observational study carried out on 100 diabetic patients newly diagnosed with diverse cancer types eligible for different chemotherapeutic protocols at the oncology unit. Urinary 8-Hydroxydeoxyguanosine level assessed at the baseline (before the required chemotherapy protocol schedule), and the 2 nd reading (at the end of the required chemotherapy protocol schedule). Results showed that there was a significant (p<0.05) increase the urinary 8-Hydroxydeoxyguanosine level between the baseline and 2 nd readings (27.04±4.33 ng/dl) vs (30.77±4.63ng/dl), and between the baseline and 2 nd readings at 7-day course (25.96±4.21ng/dl) vs (28.16±5.27ng/dl), at 14-day course (27.76±5.33 ng/dl) vs (31.56±4.47ng/dl), and at 21-day course (27.22±4.16 ng/dl) vs (31.40±4.24ng/dl). In conclusion, this study presented that oxidative stress based on elevation of the urinary 8-OHdG level is related to diabetes mellitus and cancer which is further boosted during chemotherapy administration.

show abstract

“…Для регистрации образующихся 8,5′-циклопурин-2′-деоксинуклеозидов (в том числе наиболее по-пулярных в качестве маркеров окисления ДНК и РНК 8-оксогуанозина и 8-оксо-2′-деоксигуанозина), различных видов повреждения оснований и саха-ров, одно-и двухцепочечных разрывов, сшивок и хромосомных аберраций используют биологические (иммуноферментный анализ, метод ДНК-комет, щелочная элюция) и химические методы (газовая хроматография / масс-спектрометрия и жидкостная хроматография / масс-спектрометрия с одним или двумя масс-анализаторами и использованием иониза-ции распылением в электрическом поле; жидкостная хроматография с электрохимическим или ампероме-трическим детектированием) [13,31].…”

Section: методы регистрации окислительного повреждения биомолекулunclassified

Modern Approaches to Oxidative Stress Estimation, or How to Measure the Immeasurable

Меньщикова¹,

Menshchikova²,

Zenkov³

et al. 2017

Бюллетень Восточно-Сибирского Научного Центра Сибирского Отделения Российской Академии Медицинских Наук

View full text Add to dashboard Cite

Detection and interpretation of 8-oxodG and 8-oxoGua in urine, plasma and cerebrospinal fluid

Cited by 144 publications

References 82 publications

Relationship Between Plasma 8‐OH‐Deoxyguanosine and Cardiovascular Disease and Survival in Type 2 Diabetes Mellitus: Results From the ADVANCE Trial

Relationship Between Plasma 8‐OH‐Deoxyguanosine and Cardiovascular Disease and Survival in Type 2 Diabetes Mellitus: Results From the ADVANCE Trial

Untitled

Modern Approaches to Oxidative Stress Estimation, or How to Measure the Immeasurable

Contact Info

Product

Resources

About