Detection and immunochemical characterization of a primate type C retrovirus-related p30 protein in normal human placentas.

Jerabek, L; Mellors, Robert C.; Elkon, Keith B.; Mellors, J. W.

doi:10.1073/pnas.81.20.6501

Cited by 28 publications

(12 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some of these sequences have been reported to be expressed as messenger RNA (16,22,(38)(39)(40) and also, possibly, as protein and particles (41)(42)(43)(44)(45). Antibodies to endogenous retroviruses occur in animals (46)(47)(48)(49), and the same seems to be true in humans (2,3,5).…”

Section: Discussionmentioning

confidence: 99%

Selective antibody reactivity with peptides from human endogenous retroviruses and nonviral poly(amino acids) in patients with systemic lupus erythematosus

Bengtsson

Blomberg

Nived

et al. 1996

Arthritis & Rheumatism

View full text Add to dashboard Cite

Objective. To investigate antibody responses to a broad panel of peptides derived from human endogenous retroviruses (HERVs) among unselected patients with systemic lupus erythematosus (SLE).Methods. In sera obtained from 69 patients with SLE and healthy blood donors, immunoassay was used to measure levels of antibody against synthetic peptides derived from HERVs, exogenous retroviruses, and nonviral poly(amino acids).Results. Measurement by immunoassay revealed increased frequencies of antiretroviral antibodies against 2 peptides derived from the env gene of the type C-like class, which includes ERV-9 and HERV-H, and against 2 peptides from the gag region of human T lymphotropic virus type I-related endogenous sequence 1, in patients with SLE. Antibodies to 2 nonviral peptides, polyhistidine and polyproline, were also overrepresented in patient sera. In 1 patient, longitudinal data obtained over a period of 12 years indicated that the concentrations of certain antiretroviral antibodies varied according to disease activity.

show abstract

Section: Discussionmentioning

confidence: 99%

Selective antibody reactivity with peptides from human endogenous retroviruses and nonviral poly(amino acids) in patients with systemic lupus erythematosus

Bengtsson

Blomberg

Nived

et al. 1996

Arthritis & Rheumatism

View full text Add to dashboard Cite

show abstract

“…Some associations are well known: B-cell immunoglobulinopathies (1); inflammatory diseases of the nervous system (160); autoimmune rheumatic and connective tissue diseases (94,159,180,191); and chronic fatigue immune dysfunction syndrome (40). Others have been accorded little recognition even though type C retrovirus particles occur rather abundantly in tissues, i.e., in human reproductive tissues (82,100) and psoriasis lesions (78,79). The aim of this review is to collate and appraise the data bearing on the question of whether HERVs can be implicated in specific disease entities.…”

Section: Introductionmentioning

confidence: 99%

Human endogenous retroviruses: nature, occurrence, and clinical implications in human disease

Urnovitz¹,

Murphy²

1996

Clin Microbiol Rev

175

View full text Add to dashboard Cite

Retroviral diagnostics have become standard in human laboratory medicine. While current emphasis is placed on the human exogenous viruses (human immunodeficiency virus and human T-cell leukemia virus), evidence implicating human endogenous retroviruses (HERVs) in various human disease entities continues to mount. Literature on the occurrence of HERVs in human tissues and cells was analyzed. Substantial evidence documents that retrovirus particles were clearly demonstrable in various tissues and cells in both health and disease and were abundant in the placenta and that their occurrence could be implicated in some of the reproductive diseases. The characteristics of HERVs are summarized, mechanisms of replication and regulation are outlined, and the consistent hormonal responsiveness of HERVs is noted. Clear evidence implicating HERV gene products as participants in glomerulonephritis in some cases of systemic lupus erythematosus is adduced. Data implicating HERVs as etiologic factors in reproductive diseases, in some of the autoimmune diseases, in some forms of rheumatoid arthritis and connective tissue disease, in psoriasis, and in some of the inflammatory neurologic diseases are reviewed. The current major needs are to improve methods for HERV detection, to identify the most appropriate HERV prototypes, and to develop diagnostic reagents so that the putative biologic and pathologic roles of HERVs can be better evaluated.

show abstract

“…Protein or Western blotting has been used in conjunction with immunological detection methods to investigate antigenic proteins in a number of virus studies (2,5,13). Our results showed that tile HgP0-rabbit anti-pig IgG conjugate and sera from uninfected pigs, non-specifically reacted with some of the blotted AD virus proteins.…”

Section: Discussionmentioning

confidence: 82%

Antigenically important proteins of Aujeszky's disease (pseudorabies) virus identified by immunoblotting

Todd

Hull

McNair

1987

Archives of Virology

View full text Add to dashboard Cite

Immunoblotting was used to identify those Aujeszky's disease virus proteins which elicited major antibody responses in naturally and experimentally infected pigs. Although some proteins comprising purified virus preparations reacted nonspecifically, proteins with mol. wts. of 120 K, 90 K, 71 K and 60 K were antigenically important. These corresponded in size to the virus glycoproteins identified by 3H-glucosamine labelling. Glycoproteins isolated by affinity chromatography from infected PK 15 cells solubilised by Triton X-100 (Triton-soluble glycoproteins) contained antigenic components similar in size to virus glycoproteins and produced minimal non-specific immunoblotting reactions. Antibody responses to the 120 K and 71 K proteins usually occurred together and were more pronounced than responses to other proteins especially at early times postinfection.

show abstract

Detection and immunochemical characterization of a primate type C retrovirus-related p30 protein in normal human placentas.

Cited by 28 publications

References 43 publications

Selective antibody reactivity with peptides from human endogenous retroviruses and nonviral poly(amino acids) in patients with systemic lupus erythematosus

Selective antibody reactivity with peptides from human endogenous retroviruses and nonviral poly(amino acids) in patients with systemic lupus erythematosus

Human endogenous retroviruses: nature, occurrence, and clinical implications in human disease

Antigenically important proteins of Aujeszky's disease (pseudorabies) virus identified by immunoblotting

Contact Info

Product

Resources

About