Detection and immune cell response of natural maedi visna virus (MVV) infection in Indian sheep and goats

Kumar, K. Sathish; Kumar, Pawan; Kotha, Sindhoora; Valecha, Shweta; Kumar, Rahul; Singh, Vidya; Singh, Rajendra

doi:10.1016/j.micpath.2022.105467

Cited by 4 publications

(3 citation statements)

References 24 publications

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“…In MVV IHC, viral antigen was localized mainly in the cytoplasm of macrophages, confirming the results of a previous study [ 41 ]. In our study, CAEV antigen was most frequently identified in bronchial epithelial cells with a strong cytoplasmatic and nuclear signal in areas of lymphocytic infiltration, in contrast to the findings of Storset et al [ 42 ], who detected the signal mainly in the cytoplasm of macrophages.…”

Section: Discussionsupporting

confidence: 90%

A Combined Approach for Detection of Ovine Small Ruminant Retrovirus Co-Infections

Rosato

Abril

Hilbe

et al. 2023

Viruses

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Jaagsiekte retrovirus (JSRV)-induced ovine pulmonary adenocarcinoma (OPA) is an important ovine respiratory disease in Switzerland. Furthermore, ovine lungs with OPA frequently exhibited lesions suggestive of maedi-visna virus (MVV) or caprine arthritis encephalitis virus (CAEV) infection, indicating that co-morbidities might occur. Lungs and pulmonary lymph nodes were sampled from suspected OPA cases, inflammatory lung lesions and control lungs (total of 110 cases). Tissues were (a) processed for histology and immunohistochemistry (IHC), and (b) underwent DNA extraction and real-time PCR for JSRV, MVV and CAEV. Peptide sequences were used to generate virus-specific customized polyclonal antibodies. PCR-positive OPA cases and formalin-fixed and paraffin-embedded MVV- and CAEV-infected synovial cell pellets served as positive controls. Fifty-two lungs were histologically diagnosed with OPA. Histological evidence of MVV/CAEV infection was detected in 25 lungs. JSRV was detected by PCR in 84% of the suspected OPA cases; six were co-infected with MVV and one with CAEV. MVV was detected by PCR in 14 cases, and four lungs were positive for CAEV. Three lungs had MVV/CAEV co-infection. In IHC, JSRV was detected in 91% of the PCR-positive cases, whereas MVV and CAEV immunoreactivity was seen in all PCR-positive lungs. Although PCR showed a higher sensitivity compared to IHC, the combined approach allows for investigations on viral cell tropism and pathogenic processes in co-morbidities, including their potential interdependency. Furthermore, an immunohistochemical tool for specific differentiation of MVV and/or CAEV infection was implemented.

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Section: Discussionsupporting

confidence: 90%

A Combined Approach for Detection of Ovine Small Ruminant Retrovirus Co-Infections

Rosato

Abril

Hilbe

et al. 2023

Viruses

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“…The SRLV infection has been reported worldwide with high variation of prevalence, including in Egypt (8.52%) 9 , Mexico (0.4%) 10 , Southern Spain (23.22%) 11 , India (19.58%) 12 , Japan (10.0%) 13 , South Korea (2.73%) 14 , and Thailand (2.60%) 15 .…”

Section: Introductionmentioning

confidence: 99%

Development of in-house ELISA based on recombinant gag proteins of small ruminant lentiviruses isolated from goats in Thailand

Mongkonwattanaporn,

Lertwatcharasarakul,

Rukkwamsuk

2024

Sci Rep

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Small ruminant lentiviruses (SRLVs), are grouped in Retroviridae family, remain a significant loss in the small ruminant husbandry. As a result of unavailability of vaccine and effective treatment, the diagnosis plays a crucial role for the control of SRLV infection. However, the major challenge of diagnosis of SRLV infection is the genetic and antigenic variability of the viruses that can lead to a failure in serological detection. This study investigated the circulating strains of the viruses in goats in Thailand and an in-house ELISA was developed. The coding sequences for gag protein were optimized, synthesized, and expressed in Escherichia coli for increasing the sensitivity of ELISA test. A total of 365 serum samples were examined against the recombinant protein in an in-house ELISA. The results showed that the recombinant gag achieves 96.67% sensitivity and 93.18% specificity as compared with the commercially available ELISA test kit.

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“…It is a member of the Lentivirus genus (Pepin et al, 1998) [11] . The onset of multisystemic disease problems, such as interstitial pneumonia, arthritis, chronic mastitis, etc., causes significant economic losses associated with maedi illness (Kumar et al, 2022) [9] . The prevalence is substantially higher in farms where the practice of feeding lambs or kids pool of colostrum or milk from the tank (Gomez-Lucia et al, 2018) [7] .…”

Section: Introductionmentioning

confidence: 99%

Detection of Maedi Visna Virus (MVV) in milk samples of Indian goats

Yadav,

et al. 2024

Int. J. Adv. Biochem. Res.

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Background: Maedi is a retroviral illness with a protracted incubation period that afflicts the lungs of sheep and goats. Maedi is lymphoproliferative inflammatory disease condition affecting multiple organs including lungs, and caused by lentivirus. Aim: The aim of present study was molecular detection of MVV in milk samples. Methods: 75 goat milk samples were collected and PCR assay was performed to detect the nucleic acid of MVV in the milk samples. Results: MVV nucleic acid was detected in 2.66% (2/75) of samples. Conclusion: Milk/colostrums and infectious droplets are important modes of transmission for SRLV to susceptible animal population. Detection of MVV nucleic acid in the milk samples of Indian goats was reported first time in country.

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Detection and immune cell response of natural maedi visna virus (MVV) infection in Indian sheep and goats

Cited by 4 publications

References 24 publications

A Combined Approach for Detection of Ovine Small Ruminant Retrovirus Co-Infections

A Combined Approach for Detection of Ovine Small Ruminant Retrovirus Co-Infections

Development of in-house ELISA based on recombinant gag proteins of small ruminant lentiviruses isolated from goats in Thailand

Detection of Maedi Visna Virus (MVV) in milk samples of Indian goats

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