Detection and identification of the extended- spectrum β-lactamases (bla SHV and bla CTX –M) Klebsiella pneumonia by PCR technique

Abstract

Klebsiella pneumonia was the most important nosocomial infections pathogen. It was causing several morbidity and mortality in sick animals and human. Its identification and detection performed by usage of conventional cultural characters, biochemical tests and Polymerase chain reaction technique (PCR). One-hundred clinical samples were divided into (50) samples of sheep suffering from pneumonia and (50) samples of human with UTI, collected from different regions of Al-Diwaniyah city. Thirty-four (68%) sheep samples were positive for K. pneumonia identification, while 38 (76%) urine samples of a human with UTI cases gave K. pneumoniae isolates . The results show only (72) isolates were identification by PCR technique. Thirty isolates of human samples (78.9%) were positive for detection of bla CTX-M ESBLs, while its detection did not determine in sheep. Sixty-three isolates from total isolates were positive to detection of bla SHV, these positive isolates divided into 53 isolate for a human and 28 sheep. Molecular characterization of ESBL provide information about the prevalence of ESBL producing K. pneumoniae in Al-Diwaniyah. The aim of this study was determination of CTX-M and SHV genes presence in extended-spectrum β-lactamase (ESBL) producing Klebsiella pneumoniae.