Detection and identification of Mycobacterium directly from Bactec bottles by using a DNA-rRNA probe

Chapin-Robertson, Kimberle; Dahlberg, Suzanne E.; Waycott, Sandra; Corrales, Josephino; Kontnick, C M; Edberg, Stephen C.

doi:10.1016/0732-8893(93)90097-q

Cited by 17 publications

(10 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of 359 AFB-positive BACTEC vials, 75 of the 86 vials containing MTBC (87.2%) were identified after the initial screening of broth with probes. This percentage is much higher than the 47% reported by Evans et al (6) but lower than the 100% reported by Thompson et al (16) and Chapin-Robertson et al (3). The fact that we tested many more MTBC-positive cultures than did Thompson et al (16) or Chapin-Robertson et al (3) may explain the difference between their results and ours.…”

Section: Discussioncontrasting

confidence: 81%

“…In our experience, 99 (78.5%) of the 126 cultures containing MAC were initially positive by probe analysis. This percentage is much lower than the 90, 100, and 89% reported previously (3,6,16). Possible explanations for our low percentage include the GIs of falsenegative vials and the centrifugation conditions used to prepare pellets for probe analysis.…”

Section: Discussioncontrasting

confidence: 60%

See 1 more Smart Citation

Use of Gen-Probe AccuProbes to identify Mycobacterium avium complex, Mycobacterium tuberculosis complex, Mycobacterium kansasii, and Mycobacterium gordonae directly from BACTEC TB broth cultures

1994

View full text Add to dashboard Cite

To evaluate the utility of Gen-Probe AccuProbes for the identification of mycobacteria directly from BACTEC TB 12B vials containing acid-fast bacilli, culture results for 11,375 clinical specimens other than blood received from 1 January 1992 to 30 September 1993 were reviewed retrospectively. During this period, a total of 359 of 11,375 BACTEC vials were positive for acid-fast bacilli and were evaluated for mycobacteria with one or more probes: 224 were probed for Mycobacterium tuberculosis complex, 253 were probed for Mycobacterium avium complex, 64 were probed for Mycobacterium kansasii, and 77 were probed for Mycobacterium gordonae. After initial testing with the probes, 75 vials were positive for M. tuberculosis complex, 99 were positive for M. avium complex, 11 were positive for M. kansasii, and 55 were positive for M. gordonae. Repeat testing of vials that were initially probe negative or testing of colonies from subcultures of these vials identified an additional 11 M. tuberculosis, 27 M. avium complex, 1 M. kansasii, and 9 M. gordonae that were not detected on initial screening. On the basis of these data, the percentage of organisms identified directly from the BACTEC TB 12B vials upon initial screening with each of the four AccuProbes was 87.2% for M. tuberculosis complex, 78.6% for M. avium complex, 91.7% for M. kansasii, and 85.9%o for M. gordonae.

show abstract

Section: Discussioncontrasting

confidence: 81%

Section: Discussioncontrasting

confidence: 60%

Use of Gen-Probe AccuProbes to identify Mycobacterium avium complex, Mycobacterium tuberculosis complex, Mycobacterium kansasii, and Mycobacterium gordonae directly from BACTEC TB broth cultures

1994

View full text Add to dashboard Cite

show abstract

“…Nine per cent of 750 Bactec 13A blood culture bottles were positive for mycobacteria. This recovery rate is similar to those reported, ranging from 7% to 13% [9,10]. As previously observed [3,11], MAC was the most commonly recovered mycobacterium in our study (74%) and M. genavense was recovered in only 5% of positive samples, when considering only a 6-week incubation.…”

Section: Discussionsupporting

confidence: 92%

The detection of mycobacteria in blood cultures using the Bactec system: 6 weeks versus 12 weeks of incubation? routine terminal Ziehl—Neelsen?

Greub¹,

Jaton²,

Beer³

et al. 1998

Clinical Microbiology and Infection

View full text Add to dashboard Cite

Methods: In this prospective study, all blood cultures submitted for mycobacteria detection, taken mainly from HIV‐infected patients, were incubated for 12 weeks. The clinical impact of a late positive blood culture result was assessed retrospectively. Results: From a total of 750 blood cultures, 68 had a growth index (GI) >10 due to the presence of mycobacteria. Of 545 negative blood cultures with a GI <10 within 12 weeks examined by Ziehl—Neelsen, one bottle revealed acid‐fast bacilli further identified as Mycobacterium genavense by PCR‐restriction fragment length polymorphism analysis of the hsp65 gene. For six of 39 patients with positive blood cultures, the delay to positivity was > 6 weeks (one M. tuberculosis, three M. genavense, two M. avium intracellulare complex). The prolonged incubation and the systematic terminal Ziehl—Neelsen increased the recovery of M. genavense from 5% to 14.5%. However, for only three patients did the late microbiological result lead to the introduction of antimycobacterial therapy. Conclusions: Neither a prolonged incubation longer than 6 weeks nor a terminal Ziehl—Neelsen‐stained smear of the negative blood cultures at 12 weeks seem to be clinically justified.

show abstract

“…In the majority of these, the commercial AccuProbe was used [8][9][10]; the reported sensitivities ranged from 33% to 83%. In only one study were nine of nine positive cultures detected [11]. A commercial amplification assay (Amplicor MTB; Roche Molecular Systems, Somerville, NJ, USA) has also been investigated for the same purpose; the sensitivities obtained ranged from 66% to 100% [12][13][14].…”

Section: Resultsmentioning

confidence: 99%

Early identification of Mycobacterium tuberculosis complex in BACTEC cultures by ligase chain reaction

Wang

Tay

2002

Journal of Medical Microbiology

View full text Add to dashboard Cite

A total of 1431 acid-fast bacilli (AFB) in Bactec 1 culture vials from 1427 patients was differentiated by the Bactec NAP 1 method and tested by the LCx Mycobacterium tuberculosis ligase chain reaction system. In all, 1321 of 1325 M. tuberculosis complex (MTBC) isolates were correctly detected by the LCx assay. All the 106 non-tuberculous mycobacteria (NTM) isolates were negative by the LCx assay. No false MTBC-positive result was obtained from NTM isolates. However, the LCx assay failed to detect four MTBC isolates from one patient. Sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) were 99.7, 100%, 100% and 96.4%, respectively. These data suggest that the LCx system can be used to identify MTBC in AFB-positive Bactec broth cultures when the growth index is >100. The method gives a 100% PPV and allows a faster turnaround time for MTBC than the NAP test.

show abstract

Detection and identification of Mycobacterium directly from Bactec bottles by using a DNA-rRNA probe

Cited by 17 publications

References 14 publications

Use of Gen-Probe AccuProbes to identify Mycobacterium avium complex, Mycobacterium tuberculosis complex, Mycobacterium kansasii, and Mycobacterium gordonae directly from BACTEC TB broth cultures

Use of Gen-Probe AccuProbes to identify Mycobacterium avium complex, Mycobacterium tuberculosis complex, Mycobacterium kansasii, and Mycobacterium gordonae directly from BACTEC TB broth cultures

The detection of mycobacteria in blood cultures using the Bactec system: 6 weeks versus 12 weeks of incubation? routine terminal Ziehl—Neelsen?

Early identification of Mycobacterium tuberculosis complex in BACTEC cultures by ligase chain reaction

Contact Info

Product

Resources

About