Detection and identification of equine Theileria and Babesia species by reverse line blotting: epidemiological survey and phylogenetic analysis

Sequence heterogeneity in the 18S rRNA gene within Theileria equi and Babesia caballi from horses in South Africa

“…equi sequence from South Africa (accession number: Z15105) (Allsopp et al, 1994 Nagore et al (2004a) …”

“…Subsequently, other studies led to the identification of two genetically distinct Theileria and two other Babesia genotypes infecting Spanish horses (Criado-Fornelio et al, 2004 andNagore et al, 2004a). Another study (Criado et al, 2006) showed genetically distinct T. equi-like isolates.…”

“…Advances in molecular biological techniques have resulted in the improved detection, identification and genetic characterization of many haemoparasites (Caccio et al, 2000 andNagore et al, 2004a). PCR has been applied for the detection of many species of Babesia and Theileria and has been shown to have higher sensitivity and specificity compared with serological assays (Geysen et al, 2003, Buling et al, 2007, Jefferies et al, 2007and Sibeko et al, 2008.…”

“…A sensitive and specific reverse line blot (RLB) hybridization assay, based on a sequence specific PCR that targets parasite 18S rRNA genes, has been developed for detection of Theileria and Babesia infections, including piroplasmosis in horses (Gubbels et al, 1999 andNagore et al, 2004a). This assay enables the identification of mixed infections and has also proven to be a valuable tool in the identification of novel piroplasm species or genotypes (Georges et al, 2001, Criado-Fornelio et al, 2004, Nagore et al, 2004a, Nagore et al, 2004band Nijhof et al, 2005.…”

“…This assay enables the identification of mixed infections and has also proven to be a valuable tool in the identification of novel piroplasm species or genotypes (Georges et al, 2001, Criado-Fornelio et al, 2004, Nagore et al, 2004a, Nagore et al, 2004band Nijhof et al, 2005.…”