Detection and Genotyping of Viral Gastroenteritis in Hospitalized Children Below Five Years Old in Cairo, Egypt

Abstract

This work aimed to study the detection and genotyping of rotavirus, enteric adenovirus, and astrovirus in Egyptian hospitalized children below five years old, associated with non-bacterial diarrhea. In total, 119 fecal samples were obtained from the target population, admitted to Abu El-reesh hospital in Cairo, Egypt. Based on the findings, the detection rate of at least one viral infection was 36.7% in children below five years old, whereas, the overall detection rate of rotavirus, adenovirus, and coinfection was 31%, 6.7%, and 0.8%, respectively. No astrovirus infection was observed. Spring was the peak season for rotavirus and enteric adenovirus. The findings showed that higher rates of rotavirus (78%) and enteric adenovirus (100%) were identified in children less than two years of age. The dominant genotypes were G1P [8] (29.7%), G3P [8] (27%), and G1P [4] (18.9%) for rotaviruses and genotype 41 for enteric adenoviruses. Uncommon genotypes G1P [6] and G9P [8] were also detected in five (13.5%) and four (10.8%) samples, respectively. The present findings indicated that the high incidence of rotavirus and adenovirus in children below two years old. Thus, highlighting the necessity of vaccine development to reduce the incidence of acute viral gastroenteritis in Egypt.

Genotyping and interaction-reality of Acanthamoeba, enteric adenovirus and rotavirus in drinking water, Egypt.

“…Our findings for rotavirus were relatively similar to the previous reports conducted in Egypt (8.3%), South Africa (14.9) and Brazil (15.7%) (Rizk and Allayeh, 2018;Chigor and Okoh, 2012;Miagostovich et al, 2014) and disagree with the previously reports conducted globally in drinking water samples such as; China (22.4%), Ghana (48%), and Italy (37.5%) (Glass et al, 1996;Sumi et al, 2013;Grassi et al, 2010). As well our results for enteric adenovirus were very low in contrast with the previous studies conducted in sewage (10.4%) and stool samples (6.7%) collected from Egypt according to previous studies (Kamel et al, 2010;Allayeh et al, 2018) and globally as West Africa (12.9%) (Verheyen et al, 2009). These comprehensive data for identification the prevalence of different pathogens in waters from several governorates in Egypt indicated the low prevalence of viral contamination in contrast with Acanthamoeba contamination.…”

“…The present study indicated the wet seasons (winter and autumn) were the peak for rotavirus and adenovirus. This result was similar with the previous report conducted in Egypt about the incidence of both rotavirus and adenovirus (Allayeh et al, 2018).…”

“…In the present study, adenovirus type 41 (Ad41) and rotavirus G1P[8] and G2P[4] were the most common predominant genotype identified in the examined tap water and uncommon genotypes were also identified such as G4P[8] and G9P [8]. The results were completely in agreement with the previous studies reported that Ad41,G1P[8] and G2P[4] were the most common genotypes for adenovirus and rotavirus in Egypt, respectively (Kamel et al, 2010;El-Senousy et al, 2013;Rizk and Allayeh, 2018;Allayeh et al, 2018).…”

The effect of age, gender, type of feeding and receiving a treatment on the distribution of Enterovirus infection among children in Al-Diwaniyah city, Iraq

“…This result corresponds to the researcher's results, Allayeh et al [27] and this is in line with the results of many studies conducted inside Iraq and outside it, which confirms that the female infection rate is higher than the male infection rate. Temu et al [30] indicated that the female infection rate is 23%, which is slightly higher than the male infection rate which reached 19%.…”

“…This result coincided with the result of the researcher, AL-Jeboury [26] in Babylon Governorate, where the highest incidence of intestinal viruses was recorded among children aged (2-3 years). It also coincided with the results of the researcher ,Allayeh et al [27] in Egypt, who found in their results that the highest incidence of viral intestinal infection in relation to (Rota, Astro and Adenovirus) was in the age group of less than one year, as well as consistent with the results of the researcher in Thi Qar, where the highest incidence of injury in the age group was less than two years, which was estimated at (60%) [28].…”