Detection and Genotyping of Human Papillomavirus DNA in Formalin-Fixed Paraffin-Embedded Specimens with the HPV Direct Flow CHIP System

Herraez-Hernandez, Elsa; Preda, Ovidiu; Alonso, Sonia; Pardo, Rosario Serrano; Olmo, Alberto

doi:10.2174/1874357920130927004

Cited by 15 publications

(11 citation statements)

References 10 publications

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“…Nowadays, wide spectrum of diagnostic HPV tests are available on the market, used in routine diagnostics, such as INNO-LiPA, Linear Array HPV Genotyping test, PapilloCheck HPV test, Digene Hybrid Capture 2, CLART HPV2, etc. Nevertheless, these methods show wide differences regarding genotypespeci c sensitivity and sample types that are validated in the applied method [29][30][31][32].…”

Section: Discussionmentioning

confidence: 99%

Wide Spectrum Analysis of HPV Genotypes in External Anogenital Warts among Hungarian Patients

Rideg

Oszter

Makk

et al. 2021

Preprint

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Background: External anogenital warts (EGW) are primarily associated with the low-risk Human papillomavirus (HPV) genotype 6 and 11, though coinfection with other low-risk and oncogenic high-risk HPV genotypes also occurs. Though the many studies about HPV-associated disease, the epidemiology, and the prevalence of HPV genotypes associated with EGW are not well characterized. The objective of our study was to determine the incidence of HPV genotypes in a Hungarian cohort diagnosed with EGW.Methods: A total number of 94 patients’ archived formalin-fixed paraffin-embedded (FFPE) blocks were enrolled in our retrospective study. HPV genotypes were determined, applying HPV Direct Flow Chip Test.Results: The overall prevalence of HPV DNA in EGW cases was 100%. 72.34% of the cases were mono while 27.65% were multi infections. Cumulative prevalence of HPV 6 and 11 was 98.93%; while a total of 19.4% of the cases occurred with at least one high-risk genotype. The most frequent HPV genotypes were HPV 6 (86.17%), 11 (12.76%), 42 (6.38%) followed by HPV 40, 56, 59, 66, 73 (3.19%).Conclusion: Data of our study could provide invaluable information concerning the relative frequencies of HPV types in the Hungarian population, enabling improved assessment of the actual and future efficacy of vaccination programs and forecast changes in infection patterns.

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Section: Discussionmentioning

confidence: 99%

Wide Spectrum Analysis of HPV Genotypes in External Anogenital Warts among Hungarian Patients

Rideg

Oszter

Makk

et al. 2021

Preprint

View full text Add to dashboard Cite

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“…For the HPV testing, specimens were analyzed by the HPV Direct Flow Chip system, which is intended for simultaneous detection and genotyping of 36 HPV types (high-risk HPV [16,18,26 , by direct PCR, followed by reverse dot blot automatic hybridization, based on DNA-flow technology and colorimetric detection with the automatic e-BRID R System [13]. The amplification was carried out by PCR from crude-cell extracts, by using the PCR Mix and Phire Hot Start II DNA Polymerase (MAD-003930MU-P-E-30, Master Diagnostica, Granada, Spain).…”

Section: Data Collectionmentioning

confidence: 99%

“…HPV chip membranes contained immobilized probes for hybridization control, beta-globin gene (for quality control of amplification), HPV-consensus sequences and genotype-specific HPV detection. The hybridization was performed automatically in sets of 15 samples using the e-BRID System (Master Diagnostica) [13,14]. HPV Direct Flow Chip had a sensibility of 100% and a specificity of 100% [15].…”

Section: Data Collectionmentioning

confidence: 99%

Prevalence of HPV Among HIV-Negative Women of Child-Bearing Age in Lomé, Togo

et al. 2019

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Aim: This study aimed to assess the prevalence and the distribution of HPV genotypes among HIV-negative women of child-bearing age in Lomé, Togo. Materials & methods: From April 2014 to September 2015, a cross-sectional study was conducted among HIV-negative women attending gynecological consultation in six health centers in Lomé. Cervical swabs were obtained from 324 women. HPV test was performed using HPV Direct Flow Chip. Results: The prevalence of any type and oncogenic HPV was 9.3 and 8.3%, respectively. A total of 13 different genotypes HPV, high risk (16, 18, 35, 45, 52, 53, 68, 82) and low risk (6, 40, 43, 44/65, 62/81), were found. Conclusion: Findings from this study provide essential insights for planning future public health strategies, including HPV vaccination programs.

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“…Muestra una elevada sensibilidad clínica, por lo que es adecuada para su utilización en el cribado poblacional. En los resultados positivos no se han observado reacciones cruzadas con VPH-BR 191 .…”

Section: Cobasunclassified

Guía de cribado del cáncer de cuello de útero en España, 2014

Bladé

Saladrigues

Gimferrer³

et al. 2014

Progresos de Obstetricia y Ginecología

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Justificación de la Guía de cribado del cáncer de cuello de útero en España, 2014El cáncer de cuello uterino (CCU) es la tercera neoplasia más frecuente en el mundo en las mujeres. El cribado de mujeres sanas mediante citología cervical ha demostrado claramente su eficacia, puesto que su aplicación de forma adecuada y sistemática en determinados paí-ses ha conseguido reducir en un 70-80% la incidencia y mortalidad por CCU. Este beneficio se debe a la detección de lesiones premalignas asintomáticas cuyo diagnóstico y tratamiento evita su progresión a carcinoma invasor.En las dos últimas décadas, múltiples estudios han aportado una só-lida evidencia que confirma al virus del papiloma humano (VPH) como agente causal de la práctica totalidad de los casos de CCU y de sus lesiones precursoras. Un número limitado de genotipos de VPH de alto riesgo oncogénico (VPH-AR) está causalmente implicado. Concretamente, los VPH 16 y 18 explican el 70% de los CCU y otros 10 tipos (VPH 45,31,33,52,58,35,59, 56, 51 y 39) explican el 25-35% de los casos restantes. Esta información ha permitido establecer un nuevo modelo de carcinogénesis basado en la persistencia de la infección por VPH como elemento necesario para el desarrollo de lesiones precursoras y CCU. Sin embargo, más del 90% de las infecciones por VPH son transitorias y, por tanto, irrelevantes desde el punto de vista oncogénico. Durante los primeros años de vida sexual se observa una elevada incidencia de infección, pero la mayoría de estas infecciones son transitorias y desaparecen espontáneamente. Las mujeres mayores de 30 años experimentan una clara disminución de la prevalencia de la infección por VPH, pero un

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Detection and Genotyping of Human Papillomavirus DNA in Formalin-Fixed Paraffin-Embedded Specimens with the HPV Direct Flow CHIP System

Cited by 15 publications

References 10 publications

Wide Spectrum Analysis of HPV Genotypes in External Anogenital Warts among Hungarian Patients

Wide Spectrum Analysis of HPV Genotypes in External Anogenital Warts among Hungarian Patients

Prevalence of HPV Among HIV-Negative Women of Child-Bearing Age in Lomé, Togo

Guía de cribado del cáncer de cuello de útero en España, 2014

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