Detection and genome characterization of <i>Potato virus</i> Y isolates infecting potato (<i>Solanum tuberosum</i> L.) in La Union (Antioquia, Colombia)

Abstract

Potato virus Y (PVY) is one of the most severe viruses affecting the production of potato (Solanum tuberosum) in the world. This study presents a detailed molecular analysis using nextgeneration sequencing (NGS), IC-RT-qPCR and RT-PCR on the PVY isolates infecting seed-tubers and foliage of potato plants cv. Diacol-Capiro in La Union (Antioquia, Colombia). Analysis of incidence by IC-RT-qPCR in 15 random leaf samples of three cultivation plots and fifteen sprouting tuber eye-buds reveal infection levels between 13.4 and 80%; a higher incidence of 86.7% was observed in seed-tuber samples with threshold cycle (Ct) values as low as 24.3. Genome assembly from a bulk of foliage samples resulted in a consensus PVY genome (PVY_LaUnionF) of 9,702 nt and 399 polymorphic sites within the polyprotein ORF; while the assembled genome from sprouts of tubers has 9,704 nt (PVY_LaUnionT) and contained only six polymorphic nucleotide sites. Phylogenetic analysis demonstrates that the PVY isolates from leaf samples are in the recombinant PVYNTN group (sequence identity &gt;99%); while those from tuber sprouts are in the PVYN/NTN group with identities above 95%. Sanger sequencing of viral capsid suggests the presence of a third variant related to PVYO, a prevalent strain reported in potato fields worldwide.

Pyracantha as a promising functional food: A comprehensive review on bioactive characteristics, pharmacological activity, and industrial applications

“…To treat bodily pain, Pyracantha roots are boiled and harvested in the Limpopo Province of South Africa (Semenya et al., 2012). Pyracantha is also applied in bouquets owing to its beautiful and charming foliage and berries (Gutiérrez et al., 2007). In the environment, it can be used not only for biological monitoring of copper and nickel but also to prevent land loss (Olmez et al., 2007).…”