Detection and genetic characterization of the novel torque teno virus group 6 in Taiwanese general population

Hsiao, Kuang-Liang; Wang, Li-Yu; Cheng, Ju‐Chien; Cheng, Yu-Jung; Lin, Chiung‐Ling; Liu, Hsin‐Fu

doi:10.1098/rsos.210938

Cited by 7 publications

(3 citation statements)

References 54 publications

(79 reference statements)

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“…Since TTV was first detected in 1997 in a Japanese patient who died from post-transfusion hepatitis, the disease has been detected in humans worldwide. It is the most common component of the human blood virome ( 1 – 3 ). The first time, TTVs were found in the serum of a patient with non-A-E hepatitis, but later, TTVs were found in various human tissues ( 4 ).…”

Section: Introductionmentioning

confidence: 99%

Coinfection of torque teno virus (TTV) and human papillomavirus (HPV) in cervical samples of women living in Tehran, Iran

Siahpoush¹,

Noorbazargan²,

Kalantari³

et al. 2022

IJM

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Background and Objectives: Torque Teno virus or transfusion-transmitted virus (TTV) is a non-enveloped virus with a single strand circular DNA genome that currently is classified in the Alphatorquevirus genus and the family of Anelloviridae. Unlike other DNA viruses, TTV has an extremely wide genomic diversity. This virus, based on previous studies, infects both healthy people, as well as those who have HCV and human papillomavirus (HPV). This study aimed to evaluate the coinfec- tion of torque teno virus (TTV) and HPV in cervical samples from Iranian women. Materials and Methods: In this case-control study, the fresh cervical cytobrush specimens were collected from 150 women referred to Dena laboratory in Tehran. Viral DNA was extracted from samples. The HPV-DNA was detected and genotyped. Then, nested polymerase chain reaction (Nested PCR) was performed for TTV using specific primers. Results: Among 50 cervical specimens without HPV, 14 were TTV positive (28%); among 50 low-risk HPV cervical spec- imen, 23 were TTV positive (46%), and from 50 high-risk HPV cervical specimen, 48 were TTV positive (96%). There is a significantly higher prevalence of TTV virus in low-risk and high-risk papillomavirus-infected specimens than in healthy specimens (p 0.0001). Additionally, TTV is more prevalent in samples containing high-risk papillomaviruses than in samples with low-risk papillomaviruses (P = 0.048). Conclusion: The higher prevalence of TTV among people infected with papillomavirus than in non-infected people indi- cates that both viruses are transmitted by the same mechanism (sexual route). In addition, the prevalence of TTV in samples containing high-risk papillomavirus is significantly higher than that in samples containing low-risk papillomavirus. The presence of papillomaviruses, particularly high-risk types, may be associated with TTV proliferation, which requires further research in the future.

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Section: Introductionmentioning

confidence: 99%

Coinfection of torque teno virus (TTV) and human papillomavirus (HPV) in cervical samples of women living in Tehran, Iran

Siahpoush¹,

Noorbazargan²,

Kalantari³

et al. 2022

IJM

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“…First discovered in a serum samples from Hepatitis B (HBV) and Hepatitis C (HCV) patients ( Khudair et al, 2019 ), TTV are single-stranded circular DNA (ssDNA) viruses, with a ~ 3.8 kb genome size and are currently classified into the Anelloviridae family under the genus Alphatorquevirus ( Lolomadze and Rebrikov, 2020 ). TTV are known to be a diverse viral group with over 20 genotypes and 40% viral genome heterogeneity with frequent recombination in the N22 region of the open reading frame (ORF) 1 gene ( Manni et al, 2002 ; Hino and Miyata, 2007 ; Hsiao et al, 2021 ). The TTV-like mini virus (TTMV) was added to the TTV group in 2000 ( Takahashi et al, 2000 ) and has a genome size of ~2.9 kb ( Hino and Miyata, 2007 ).…”

Section: Discussionmentioning

confidence: 99%

Comparison of de novo assembly using long-read shotgun metagenomic sequencing of viruses in fecal and serum samples from marine mammals

Vigil,

2023

Front. Microbiol.

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IntroductionViral diseases of marine mammals are difficult to study, and this has led to a limited knowledge on emerging known and unknown viruses which are ongoing threats to animal health. Viruses are the leading cause of infectious disease-induced mass mortality events among marine mammals.MethodsIn this study, we performed viral metagenomics in stool and serum samples from California sea lions (Zalophus californianus) and bottlenose dolphins (Tursiops truncates) using long-read nanopore sequencing. Two widely used long-read de novo assemblers, Canu and Metaflye, were evaluated to assemble viral metagenomic sequencing reads from marine mammals.ResultsBoth Metaflye and Canu assembled similar viral contigs of vertebrates, such as Parvoviridae, and Poxviridae. Metaflye assembled viral contigs that aligned with one viral family that was not reproduced by Canu, while Canu assembled viral contigs that aligned with seven viral families that was not reproduced by Metaflye. Only Canu assembled viral contigs from dolphin and sea lion fecal samples that matched both protein and nucleotide RefSeq viral databases using BLASTx and BLASTn for Anelloviridae, Parvoviridae and Circoviridae families. Viral contigs assembled with Canu aligned with torque teno viruses and anelloviruses from vertebrate hosts. Viruses associated with invertebrate hosts including densoviruses, Ambidensovirus, and various Circoviridae isolates were also aligned. Some of the invertebrate and vertebrate viruses reported here are known to potentially cause mortality events and/or disease in different seals, sea stars, fish, and bivalve species.DiscussionCanu performed better by producing the most viral contigs as compared to Metaflye with assemblies aligning to both protein and nucleotide databases. This study suggests that marine mammals can be used as important sentinels to surveil marine viruses that can potentially cause diseases in vertebrate and invertebrate hosts.

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“…Anelloviruses show extreme genetic diversity. Due to frequent NGS application, many new genomic forms and genotypes are continuously being discovered [28,29]. Despite the cosmopolitan distribution of the anelloviruses, they are regarded as non-pathogenic [30].…”

Section: The Commensal Blood Donor Viromementioning

confidence: 99%

Viral Metagenomics for Identification of Emerging Viruses in Transfusion Medicine

Slavov

2022

Viruses

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Viral metagenomics has revolutionized our understanding for identification of unknown or poorly characterized viruses. For that reason, metagenomic studies gave been largely applied for virus discovery in a wide variety of clinical samples, including blood specimens. The emerging blood-transmitted virus infections represent important problem for public health, and the emergence of HIV in the 1980s is an example for the vulnerability of Blood Donation systems to such infections. When viral metagenomics is applied to blood samples, it can give a complete overview of the viral nucleic acid abundance, also named “blood virome”. Detailed characterization of the blood virome of healthy donors could identify unknown (emerging) viral genomes that might be assumed as hypothetic transfusion threats. However, it is impossible only by application of viral metagenomics to assign that one viral agent could impact blood transfusion. That said, this is a complex issue and will depend on the ability of the infectious agent to cause clinically important infection in blood recipients, the viral stability in blood derivatives and the presence of infectious viruses in blood, making possible its transmission by transfusion. This brief review summarizes information regarding the blood donor virome and some important challenges for use of viral metagenomics in hemotherapy for identification of transfusion-transmitted viruses.

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Detection and genetic characterization of the novel torque teno virus group 6 in Taiwanese general population

Cited by 7 publications

References 54 publications

Coinfection of torque teno virus (TTV) and human papillomavirus (HPV) in cervical samples of women living in Tehran, Iran

Coinfection of torque teno virus (TTV) and human papillomavirus (HPV) in cervical samples of women living in Tehran, Iran

Comparison of de novo assembly using long-read shotgun metagenomic sequencing of viruses in fecal and serum samples from marine mammals

Viral Metagenomics for Identification of Emerging Viruses in Transfusion Medicine

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