Detection and genetic characterization of a wide range of infectious agents in Ixodes pavlovskyi ticks in Western Siberia, Russia

Рар, В. А.; Ливанова, Н. Н.; Tkachev, Sergey; Kaverina, Galina; Tikunov, Artem; Sabitova, Yuliya; Igolkina, Yana; Panov, Victor V.; Ливанов, С. Г.; Фоменко, Н. В.; Бабкин, И. В.; Tikunova, Nina

doi:10.1186/s13071-017-2186-5

Cited by 56 publications

(33 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Among these species, Borrelia burgdorferi sensu stricto (s.s.) Borrelia garinii, Borrelia afzelii and “ Borrelia bavariensis ” sp. nov. are well-known causes of Lyme disease in North America, Europe (Casjens et al, 2011) and Russia (Rar et al, 2017). Borrelia finlandensis isolated from an Ixodes ricinus tick in Finland is closely related to B. burgdorferi s.s. and is suggested as candidate for new-species status (Casjens et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Borrelia burgdorferi sensu lato and Borrelia miyamotoi in ixodid ticks in the Far East of Russia

Pukhovskaya

Морозова

Высочина

et al. 2019

International Journal for Parasitology: Parasites and Wildlife

View full text Add to dashboard Cite

Borrelia burgdorferi sensu lato (s.l.) DNA was detected by PCR in Ixodes persulcatus Schulze, 1930, Haemaphysalis concinna Koch, 1844 , Haemaphysalis japonica douglasi Nuttall et Warburton, 1915 and Dermacentor silvarum Olenev, 1932 ticks collected in the Amur region, the Jewish Autonomous region, the Sakhalin region and on the Khabarovsk territory. Infection rate of I. persulcatus with B. burgdorferi s.l. 10–69% exceeded the corresponding values of three other tick species in all examined regions during 1999–2014 despite different tick abundance and dominance structure. Bacterial loads estimated on the base of quantitative real time PCR varied from 10 2 to 10 9 genome-equivalents per a tick with maximal values for I. persulcatus and H. japonica. Phylogenetic analysis of 16S rRNA gene and 5S—23S rRNA intergenic spacer nucleotide sequences revealed two species: 1) Borrelia garinii of Asian type NT29 with several isolates of European type 20047; 2) Borrelia afzelii with identical sequences of the majority of studied isolates and VS461 reference strain in all regions except the Sakhalin Island where B. afzelii was not found . Borrelia miyamotoi of the relapsing fever group was detected as monoinfection or in combination with B. burgdorferi s.l. in 4.0 ± 0.9% and 4.8 ± 0.9% I. persulcatus ticks, respectively. Multiple locus sequence analysis of three fragments of 16S rRNA, glpQ and p66 genes proved that all the Far Eastern B. miyamotoi isolates belonged to the Asian type identical to FR64b strain (GenBank CP004217 ) from Japan. Wide distribution of Borrelia DNA in ticks, relative genetic homogeneity with similar sequences of the coding regions and the intergenic spacer of Borrelia wild isolates and temporal stability with high homology levels of the Far Eastern isolates of B. garinii , B. afzelii and B. miyamotoi with previously described spirochetes from the surrounding regions of Russia, China and Japan allowed us to suggest multiple ecological niches as the stability factor of the parasitic system.

show abstract

Section: Introductionmentioning

confidence: 99%

Prevalence of Borrelia burgdorferi sensu lato and Borrelia miyamotoi in ixodid ticks in the Far East of Russia

Pukhovskaya

Морозова

Высочина

et al. 2019

International Journal for Parasitology: Parasites and Wildlife

View full text Add to dashboard Cite

show abstract

“…species complex than B. miyamotoi (Girard et al 2009, Margos et al 2011, Mechai et al 2015, Rar et al 2017). In California, prevalence of B. burgdorferi s.l.…”

mentioning

confidence: 98%

“…Globally, prevalence of B. miyamotoi in host-seeking Ixodes ticks ranges from 1 to 10%, with most local estimates falling closer to the low end (Scoles et al 2001, Barbour et al 2009, Rollend et al 2013, Sinski et al 2016, Rar et al 2017). In California, B. miyamotoi infection prevalences of 1.6 and 1.7% were reported in I. pacificus nymphs from Mendocino and Sonoma counties (Mun et al 2006, Crowder et al 2014), and 1.4% statewide (Padgett et al 2014).…”

mentioning

confidence: 99%

“…Intriguingly, Lyme disease spirochetes typically infect Ixodes nymphs with greater frequency (prevalence 3–49%) than B. miyamotoi in areas where the two pathogens are sympatric (Barbour et al 2009, Wagemakers et al 2015, Rar et al 2017) and there is considerably more genetic diversity within the B. burgdorferi s.l. species complex than B. miyamotoi (Girard et al 2009, Margos et al 2011, Mechai et al 2015, Rar et al 2017).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Prevalence and Geographic Distribution of Borrelia miyamotoi in Host-Seeking Ixodes pacificus (Acari: Ixodidae) Nymphs in Mendocino County, California

Lynn

Graham

Horiuchi

et al. 2018

Journal of Medical Entomology

View full text Add to dashboard Cite

Borrelia miyamotoi is an increasingly recognized human pathogen transmitted by Ixodes ticks in the Northern Hemisphere. In North America, infection prevalences of B. miyamotoi are characteristically low (<10%) in Ixodes scapularis (Say; Acari: Ixodidae) and Ixodes pacificus (Cooley & Kohls; Acari: Ixodidae), both of which readily bite humans. We tested 3,255 host-seeking I. pacificus nymphs collected in 2004 from 79 sites throughout Mendocino County in north-coastal California for presence of B. miyamotoi. The collection sites represented a variety of forest types ranging from hot, dry oak woodlands in the southeast, to coastal redwoods in the west, and Ponderosa pine and Douglas fir-dominated areas in the northern part of the county. We found that B. miyamotoi was geographically widespread, but infected I. pacificus nymphs infrequently (cumulative prevalence of 1.4%). Infection prevalence was not significantly associated with geographic region or woodland type, and neither density of host-seeking nymphs, nor infection with Borrelia burgdorferi sensu stricto was associated with B. miyamotoi infection status in individual ticks. Because B. burgdorferi prevalence at the same sites was previously associated with woodland type and nymphal density, our results suggest that despite sharing a common vector, the primary modes of enzootic maintenance for the two pathogens are likely different.

show abstract

“…miyamotoi has been detected in various Ixodes spp. in North America, Europe, and Russia [4, 12, 13]. In South Korea, B .…”

Section: Introductionmentioning

confidence: 99%

Detection of Borrelia miyamotoi in Ixodes nipponensis in Korea

et al. 2019

View full text Add to dashboard Cite

Background This study investigated Borrelia species prevalence in ticks from vegetation, through a molecular method, in Gwangju Metropolitan City, South Korea. Methodology/Principal findings A total of 484 ticks were collected through flagging and dragging in a suburban area of Gwangju Metropolitan City, South Korea, in 2014. These ticks were morphologically identified and subjected to nested PCR, targeting Borrelia –specific CTP synthase ( pyrG ), outer surface protein A ( ospA ) and flagellin ( flaB ) genes. Molecular biological species identification of Borrelia -positive ticks was conducted via 16S rRNA PCR assays. Of the 484 ticks collected, 417 (86.2%) were identified as Haemaphysalis longicornis , 42 (8.7%) as H . flava , and 25 (5.2%) as Ixodes nipponensis . All the ixodid ticks containing Borrelia species bacteria were confirmed to be I . nipponensis adults, by both morphological and molecular methods. Of the 25 I . nipponensis ticks collected, four (16%) were positive for Borrelia species, three of which were B . afzelii and one B . miyamotoi . Conclusions/Significance Our study has shown the harboring of B . miyamotoi by I . nipponensis in South Korea. Morphological and molecular genetic analyses revealed that, in South Korea, I . nipponensis could potentially transmit B . miyamotoi to humans.

show abstract

Detection and genetic characterization of a wide range of infectious agents in Ixodes pavlovskyi ticks in Western Siberia, Russia

Cited by 56 publications

References 69 publications

Prevalence of Borrelia burgdorferi sensu lato and Borrelia miyamotoi in ixodid ticks in the Far East of Russia

Prevalence of Borrelia burgdorferi sensu lato and Borrelia miyamotoi in ixodid ticks in the Far East of Russia

Prevalence and Geographic Distribution of Borrelia miyamotoi in Host-Seeking Ixodes pacificus (Acari: Ixodidae) Nymphs in Mendocino County, California

Detection of Borrelia miyamotoi in Ixodes nipponensis in Korea

Contact Info

Product

Resources

About