Detection and expression of genes for phosphorus metabolism in picocyanobacteria from the Laurentian Great Lakes

Kutovaya, Olga A.; McKay, R. Michael L.; Bullerjahn, George S.

doi:10.1016/j.jglr.2013.09.009

Cited by 17 publications

(9 citation statements)

References 61 publications

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“…In the marine environment, the cyanobacterium Trichodesmium erythraeum has been found to carry gene cassettes encoding the enzymes for the conversion of MPNs to CH 4 (Dyhrman et al 2006; Beversdorf et al 2010). Similar genes have been found in the freshwater cyanobacterium Picocyanobacterium (Kutovaya et al 2013; Yao et al 2016). However, in previous studies, enrichment of Lake Stechlin water with MPNs has produced conflicting results in stimulating CH 4 production (Grossart et al 2011; Bizic‐Ionescu et al 2018).…”

Section: Discussionsupporting

confidence: 77%

Photosynthesis‐driven methane production in oxic lake water as an important contributor to methane emission

Günthel

Klawonn

Woodhouse

et al. 2020

Limnology & Oceanography

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Recent discovery of methane (CH4) production in oxic waters challenges the conventional understanding of strict anoxic requirement for biological CH4 production. High‐resolution field measurements in Lake Stechlin, as well as incubation experiments, suggested that oxic‐water CH4 production occurred throughout much of the water column and was associated with phytoplankton especially diatoms, cyanobacteria, green algae, and cryptophytes. In situ concentrations and δ13C values of CH4 in oxic water were negatively correlated with soluble reactive phosphorus concentrations. Using 13C‐labeling techniques, we showed that bicarbonate was converted to CH4, and the production exceeded oxidation at day, but was comparable at night. These experimental data, along with complementary field observations, indicate a clear link between photosynthesis and the CH4 production‐consumption balance in phosphorus‐limited epilimnic waters. Comparison between surface CH4 emission data and experimental CH4 production rates suggested that the oxic CH4 source significantly contributed to surface emission in Lake Stechlin. These findings call for re‐examination of the aquatic CH4 cycle and climate predictions.

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Section: Discussionsupporting

confidence: 77%

Photosynthesis‐driven methane production in oxic lake water as an important contributor to methane emission

Günthel

Klawonn

Woodhouse

et al. 2020

Limnology & Oceanography

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“…However, RH714 failed to grow on medium supplemented with DNA (Figure ). Phosphonates, which are organic P compounds containing a covalent carbon–phosphorus (C–P) bond, are detectable components of DOP in some freshwater environments. , Several reports indicate that the genes involved in phosphonate uptake and assimilation are widespread in environments where freshwater cyanobacteria thrive. , However, two kinds of phosphonates, namely aminoethylphosphonate and methylphosphonate, were unable to support the growth of wild-type Syn 7942 or strain RH714 (Figure ), probably because of the absence of genes for phosphonate uptake and/or assimilation. To evaluate the effect of the presence of P sources in freshwater ecosystems, we performed growth assays using BG11 media prepared using sterilized pond or river water.…”

Section: Resultsmentioning

confidence: 99%

Synthetic Phosphorus Metabolic Pathway for Biosafety and Contamination Management of Cyanobacterial Cultivation

et al. 2018

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Recent progress in genetic engineering and synthetic biology have greatly expanded the production capabilities of cyanobacteria, but concerns regarding biosafety issues and the risk of contamination of cultures in outdoor culture conditions remain to be resolved. With this dual goal in mind, we applied the recently established biological containment strategy based on phosphite (HPO, Pt) dependency to the model cyanobacterium Synechococcus elongatus PCC 7942 ( Syn 7942). Pt assimilation capability was conferred on Syn 7942 by the introduction of Pt dehydrogenase (PtxD) and hypophosphite transporter (HtxBCDE) genes that allow the uptake of Pt, but not phosphate (HPO, Pi). We then identified and disrupted the two indigenous Pi transporters, pst (Synpcc7942_2441 to 2445) and pit (Synpcc7942_0184). The resultant strain failed to grow on any media containing various types of P compounds other than Pt. The strain did not yield any escape mutants for at least 28 days with a detection limit of 3.6 × 10 per colony forming unit, and rapidly lost viability in the absence of Pt. Moreover, growth competition of the Pt-dependent strain with wild-type cyanobacteria revealed that the Pt-dependent strain could dominate in cultures containing Pt as the sole P source. Because Pt is rarely available in aquatic environments this strategy can contribute to both biosafety and contamination management of genetically engineered cyanobacteria.

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“…The prokaryotic structure of the picocyanobacteria cells provides them with the minimum costs for metabolism, and this factor has been considered as the main reason for their success in oligotrophic conditions (Weisse and Kenter, 1991). Besides, the ability to adapt to low-P conditions by accessing multiple forms of organic P also contributed to their success in oligotrophic conditions (Kutovaya et al, 2013; Callieri, 2017). In a 4-year study of picophytoplankton communities in Lake Maggiore, the abundance of picocyanobacteria gradually increased as the lake’s nutrient loads declined (Stockner, 1991).…”

Section: Discussionmentioning

confidence: 99%

The Community Structure of Picophytoplankton in Lake Fuxian, a Deep and Oligotrophic Mountain Lake

Shi

et al. 2019

Front. Microbiol.

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Spatial and seasonal dynamics of picophytoplankton were investigated by flow cytometry over a year in Lake Fuxian, a deep and oligotrophic mountain lake in southwest China. The contribution of picophytoplankton to the total Chl-a biomass and primary production were 50.1 and 66.1%, respectively. Picophytoplankton were mainly composed of phycoerythrin-rich picocyanobacteria (PE-cells) and photosynthetic picoeukaryotes (PPEs). PPEs were dominant in spring, reaching a maximum cell density of 3.0 × 104 cell mL–1, while PE-cells were prevalent in other seasons. PE-cell abundance was relatively similar throughout the year, except for a decrease in summer during the stratification period, when nutrient concentration was low. High-throughput sequencing results from the sorted samples revealed that Synechococcus was the major PE-cell type, while Chrysophyceae, Dinophyceae, Chlorophyceae, Eustigmatophyceae, and Prymnesiophyceae were equally important PPEs. In spring, PPEs were mainly composed of Chlorophyceae and Trebouxiophyceae, while in summer, their dominance was replaced by that of Chrysophyceae and Prymnesiophyceae. Eustigmatophyceae and Chlorophyceae became the major PPEs in autumn, and Dinophyceae became the most abundant in winter. Single cells of Microcystis were usually detected in summer in the south, suggesting the deterioration of the water quality in Lake Fuxian.

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Detection and expression of genes for phosphorus metabolism in picocyanobacteria from the Laurentian Great Lakes

Cited by 17 publications

References 61 publications

Photosynthesis‐driven methane production in oxic lake water as an important contributor to methane emission

Photosynthesis‐driven methane production in oxic lake water as an important contributor to methane emission

Synthetic Phosphorus Metabolic Pathway for Biosafety and Contamination Management of Cyanobacterial Cultivation

The Community Structure of Picophytoplankton in Lake Fuxian, a Deep and Oligotrophic Mountain Lake

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