Detection and drug resistance profile of <i>Escherichia coli</i> from subclinical mastitis cows and water supply in dairy farms in Saraburi Province, Thailand

Hinthong, Woranich; Pumipuntu, Natapol; Santajit, Sirijan; Kulpeanprasit, Suphang; Buranasinsup, Shutipen; Sookrung, Nitat; Chaicumpa, Wanpen; Aiumurai, Pisinee; Indrawattana, Nitaya

doi:10.7717/peerj.3431

Cited by 64 publications

(50 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This indicated a wide diversity of resistance which might persist in the environment and proliferate, thus creating great chances for the spread of resistance genes via horizontal or lateral gene transfer causing negative influences on human and animal antibiotic chemotherapy [60]. The findings corroborate those of Hinthong et al [61] where 21 antibiotic-resistance patterns were elucidated. However, the overall predominant phenotypes were AP-A-GM-E-AUG, A-E-AP and A-E-TS added to C-T-E resistance patterns observed in Salmonella and E. coli species, respectively.…”

Section: Discussionsupporting

confidence: 82%

Characterisation and Antibiotic Resistance of Selected Bacterial Pathogens Recovered from Dairy Cattle Manure during Anaerobic Mono-Digestion in a Balloon-Type Digester

et al. 2018

View full text Add to dashboard Cite

Dairy cattle manure serves as a potential source of contamination and infection of animals, humans and the environment. Manure samples withdrawn from a balloon-type digester during anaerobic digestion were evaluated for the presence of antibiotic-resistant bacterial pathogens. The bacterial load of the samples was determined via a viable plate count method and the recovered isolates were subjected to characterisation and identification. These isolates were employed in antibiotic susceptibility testing using a disc diffusion method against a suite of 10 conventional antibiotics. The multiple antibiotic resistance (MAR) index was calculated and MAR phenotypes were generated. Although all the bacterial pathogens showed a certain degree of resistance to the studied antibiotics, a marked resistance was demonstrated by Campylobacter sp. to co-trimoxazole (87.5%) and nalidixic acid (81.5%). Remarkably, a high resistance (82.42%) was demonstrated against the antibiotic class, macrolide, followed by beta-lactams (40.44%), suggesting that bacterial resistance depended on the chemical structure of the antibiotics. However, individual bacterial isolates varied in resistance to particular antibiotics. Of the 83 bacterial isolates, 40(48.19%) observed MAR > 0.2 and, thus, were described as multidrug-resistant isolates. A total of 28 MAR phenotypes were revealed with the highest frequency of MAR phenotypes (37.5%) expressed against 3 antibiotics. Results indicated a high risk of exposure to various antibiotics and wide diversity of antibiotic resistance.

show abstract

Section: Discussionsupporting

confidence: 82%

Characterisation and Antibiotic Resistance of Selected Bacterial Pathogens Recovered from Dairy Cattle Manure during Anaerobic Mono-Digestion in a Balloon-Type Digester

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Indeed, isolates harboring bla CMY2 were in almost all cases, AM-resistant and FOX-susceptible or even susceptible to all blactams tested. Hinthong et al 28 detected isolates displaying the same characteristic among nonpathogenic E. coli from a water supply in Thailand. Davis et al 29 demonstrated that in the absence of a selective pressure, which can be observed in natural settings, where antibiotic presence is limited, microorganisms are more prone to accumulate mutations leading to dysfunctional pseudogenes (also antibiotic resistance pseudogene), which lost their ability to be expressed.…”

Section: Discussionmentioning

confidence: 98%

Antibiotic Susceptibility and Virulence Factors inEscherichia colifrom Sympatric Wildlife of the Apuan Alps Regional Park (Tuscany, Italy)

Turchi

Dec

Bertelloni

et al. 2019

Microbial Drug Resistance

View full text Add to dashboard Cite

Today a growing number of studies are focusing on antibiotic resistance in wildlife. This is due to the potential role of wild animals as reservoirs and spreaders of pathogenic and resistant bacteria. This study focused on isolating and identifying Escherichia coli from the feces of wild animals living in the Apuan Alps Regional Park (Tuscany, Italy) and evaluating some of their antibiotic resistance and pathogenicity traits. Eighty-five fecal samples from different species were studied. Seventy-one E. coli were identified by matrix assisted laser desorption ionization-time of flight mass spectrometry analysis, subjected to antibiograms and polymerase chain reaction for the detection of antibiotic resistance genes and pathogenicity factors. The highest resistance rates were found against cephalothin (39.4%) and ampicillin (33.8%), followed by amoxicillin/clavulanic acid (15.5%), streptomycin (12.7%), and tetracycline (5.6%). Regarding resistance genes, 39.4% of the isolates were negative for all tested genes. The remaining isolates were positive for bla CMY-2 , sul2, strA-strB and aadA1, tet(B), and tet(A), encoding resistance to beta-lactams, trimethoprim/sulfamethoxazole, streptomycin, and tetracycline, respectively. With regard to virulence factors, 63.4% of the isolates were negative for all genes; 21.1% carried astA alone, which is associated with different pathotypes, 9.9% carried both escV and eaeA (aEPEC); single isolates (1.4%) harbored escV (aEPEC), escV associated with astA and eaeA (aEPEC), astA with stx2 and hlyA (EHEC) or astA and stx1, stx2, and hlyA (EHEC). These results show that wildlife from nonanthropized environments can be a reservoir for antibiotic-resistant microorganisms and suggest the need for a deeper knowledge on their origin and diffusion mechanisms through different ecological niches.

show abstract

“…There is ample evidence that the widespread use of antibiotics in agriculture and medicine is the main reason for the high resistance rate of Gram-negative bacteria (Bothyna & Randa, 2018). Various food and environmental sources contain bacteria resistant to one or more antimicrobial agents used in human or veterinary medicine and animal food production (Hinthong, Pumipuntu, & Santajit, 2017).…”

Section: E Coli Isolated From Retail Foodsmentioning

confidence: 99%

Antimicrobial resistance of Escherichia coli isolated from retail foods in northern Xinjiang, China

Zhang

Luo

et al. 2020

Food Science & Nutrition

View full text Add to dashboard Cite

To determine antimicrobial resistance, 431 samples of retail foods purchased at different supermarkets in Northern Xinjiang were examined in this study. There were 112 Escherichia coli strains that were isolated, with approximately 26% of the samples contaminated by E. coli. The detection rate of E. coli isolated from pork was the highest (59.6%), followed by mutton (52.6%), retail fresh milk (52.4%), duck (36.4%), beef (35.3%), chicken (33.3%), and ready-to-eat food (12.9%); the E. coli detection rate for fish and vegetables was <11%. The result showed that the 112 isolates were mostly resistant to tetracycline (52%), followed by ampicillin (42%), compound trimethoprim/sulfamethoxazole (37%), amoxicillin (33%), and nalidixic acid (32%), imipenem resistance was not detected. One hundred isolates carried at least one antimicrobial resistance gene. The detection rate of resistance genes of our study was as follows:tetA (38%), tetB (27%), bla OXA (40%), bla TEM (20%), floR (20%), sul1 (16%), sul2 (27%), aad Ala (19%), aadB (11%), strA (28%), and strB (24%); tetC and bla PSE were not detected. Virulence genes fimC, agg, stx2, fimA, fyuA, papA, stx1, and eaeA were found in 52, 34, 21, 19, 6, 3, 2, and 2 isolates, respectively; papC was not detected. There was a statistically significant association between fimC and resistance to ciprofloxacin (p = .001), gentamicin (p = .001), amikacin (p = .001), levofloxacin (p = .001), and streptomycin (p = .001); between fimA and resistance to tetracycline (p = .001), ampicillin (p = .001), compound trimethoprim/sulfamethoxazole (p = .001), and amoxicillin (p = .003); between agg and resistance to gentamicin (p = .001), tetracycline (p = .001), ciprofloxacin (p = .017), and levofloxacin (p = .001); and between stx2 and resistance to ampicillin (p = .001), tetracycline (p = .001), compound trimethoprim/ sulfamethoxazole (p = .002), and amoxicillin (p = .015).

show abstract

Detection and drug resistance profile of Escherichia coli from subclinical mastitis cows and water supply in dairy farms in Saraburi Province, Thailand

Cited by 64 publications

References 36 publications

Characterisation and Antibiotic Resistance of Selected Bacterial Pathogens Recovered from Dairy Cattle Manure during Anaerobic Mono-Digestion in a Balloon-Type Digester

Characterisation and Antibiotic Resistance of Selected Bacterial Pathogens Recovered from Dairy Cattle Manure during Anaerobic Mono-Digestion in a Balloon-Type Digester

Antibiotic Susceptibility and Virulence Factors inEscherichia colifrom Sympatric Wildlife of the Apuan Alps Regional Park (Tuscany, Italy)

Antimicrobial resistance of Escherichia coli isolated from retail foods in northern Xinjiang, China

Contact Info

Product

Resources

About