Detection and differentiation of <i>Fusarium oxysporum</i>
 f. sp. <i>lycopersici</i>
 race 1 using loop-mediated isothermal amplification with three primer sets

Ayukawa, Yu; Komatsu, Ken; Kashiwa, Takeshi; Akai, Kotaro; Yamada, Maki; Teraoka, Tohru; Arie, Tsutomu

doi:10.1111/lam.12597

Cited by 21 publications

(32 citation statements)

References 21 publications

(61 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our present method is unable to distinguish race 1 and race 2, but we have previously developed conventional LAMP to discriminate Fol race 1 from other races by detection of SIX4 , which is unique to Fol race 1 18 . Combination of both our LAMP methods can identify almost all Fol races.…”

Section: Discussionmentioning

confidence: 88%

“…18 SIX4-FIPGCCTCCTTGTCATCTACCGCATTTGGGGTGATAGAGAGCCTGAyukawa et al . 18 SIX4-BIPATGGCAAAGTTGTCACGCGTGTTGGGCCTTGAGTCGAATGAyukawa et al . 18 SIX4-LFGACGGAGAGCAAGTAGCGTAyukawa et al .…”

Section: Resultsmentioning

confidence: 99%

“…18 SIX4-LFGACGGAGAGCAAGTAGCGTAyukawa et al . 18 SIX4-LBCAGGAAAGCCAGGAATAGGACGAAyukawa et al . 18

*3′ ends of joint DNAs consist of UQprobe-G complementary sequences

…”

Section: Resultsmentioning

confidence: 99%

“…For this purpose, we employed the conventional LAMP developed in the previous study 18 , as well as the conventional PCR. LAMP products with the SIX4 primer set were obtained in three independent reactions when Soil DNA 1 and DNA 2 were used as a template (Table 3, Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

“…This allowed discrimination between Fol race 1 and other races by detection of SIX4 , as well as identification of Fol race 3 by primers that can detect either of the three types of single nucleotide substitutions (G121A, G134A or G137C) in SIX3 . To improve rapidity and sensitivity of Fol race distinction, we have recently developed a loop-mediated isothermal amplification (LAMP) method targeting SIX4 unique to race 1 18 . However, LAMP has never been applied to the discrimination of Fol race 3 from other races, which needs detection of point mutations in SIX3 .…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi

Ayukawa

Hanyuda

Fujita

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Tomato wilt pathogen Fusarium oxysporum f. sp. lycopersici (Fol) is grouped into three races based on their pathogenicity to different host cultivars. Rapid detection and discrimination of Fol races in field soils is important to prevent tomato wilt disease. Although five types of point mutations in secreted in xylem 3 (SIX3) gene, which are characteristic of race 3, have been reported as a molecular marker for the race, detection of these point mutations is laborious. The aim of this study is to develop a rapid and accurate method for the detection of point mutations in SIX3 of Fol. Loop-mediated isothermal amplification (LAMP) of SIX3 gene with the universal QProbe as well as two joint DNAs followed by annealing curve analysis allowed us to specifically detect Fol and discriminate race 3 among other races in about one hour. Our developed method is applicable for detection of races of other plant pathogenic fungi as well as their pesticide-resistant mutants that arise through point mutations in a particular gene.

show abstract

Section: Discussionmentioning

confidence: 88%

“…18 SIX4-FIPGCCTCCTTGTCATCTACCGCATTTGGGGTGATAGAGAGCCTGAyukawa et al . 18 SIX4-BIPATGGCAAAGTTGTCACGCGTGTTGGGCCTTGAGTCGAATGAyukawa et al . 18 SIX4-LFGACGGAGAGCAAGTAGCGTAyukawa et al .…”

Section: Resultsmentioning

confidence: 99%