Detection and differential diagnosis of colon cancer by a cumulative analysis of promoter methylation

Yang, Qiong; Dong, Ying; Wu, Wei; Zhu, Chunlei; Chong, Hui; Lu, Jie; Yu, Dehai; Liu, Libing; Lv, Fengting; Wang, Shu

doi:10.1038/ncomms2209

Cited by 70 publications

(68 citation statements)

References 35 publications

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“…Previous work by the Wang research group used cationic conjugated polymers (CCP) as the energy donor to quantitatively detect gene methylation and achieve high sensitivity for colon cancer detection (Feng et al, 2008(Feng et al, , 2010Yang et al, 2012;Zhang et al, 2014). When compared with organic fluorophores, QDs have several advantages, including a narrow emission spectrum, broad absorption spectrum, negligible photobleaching, and high photochemical stability Clapp et al, 2006;ReschGenger et al, 2008;Hötzer et al, 2012;Su et al, 2014).…”

Section: Principle and Feature Of Qds-based Fret Methods For Methylatimentioning

confidence: 99%

A panel of promoter methylation markers for invasive and noninvasive early detection of NSCLC using a quantum dots-based FRET approach

Bai

Mao

et al. 2016

Biosensors and Bioelectronics

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Non-small-cell lung cancer (NSCLC) leads to a significant proportion of cancer-related deaths, and early detection of NSCLC can significantly increase cancer survival rates. A promising approach has been studied to exploit DNA methylation, which is closely correlated to early cancer diagnosis. Herein, in order to realize the early detection of NSCLC, we utilized the developed quantum dots-based (QDs-based) fluorescence resonance energy transfer (FRET) nanosensor technique to analyze the promoter methylation in early stage NSCLC tissue samples and noninvasive bronchial brushing specimens. Using this method, the methylation levels can be quantitatively determined by measuring the signal amplification during FRET. A panel of three tumor suppressor genes (PCDHGB6, HOXA9 and RASSF1A) was assessed in 50 paired early stage NSCLC and their adjacent nontumorous tissue (NT) samples, and 50 early stage NSCLC bronchial brushing and normal specimens. The combined detection was able to identify not only tissue samples but noninvasive bronchial brushing specimens from control cases with a high degree of sensitivity of 92% (AUC=0.977, P<0.001) and 80% (AUC=0.907, P<0.001) respectively, indicating the versatility of promoter expression in invasive and noninvasive NSCLC samples. Therefore this approach can be used to sensitively analyze the methylation levels of cancer-related genes, which might be a potential tool for noninvasive early clinical diagnosis of cancers.

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Section: Principle and Feature Of Qds-based Fret Methods For Methylatimentioning

confidence: 99%

A panel of promoter methylation markers for invasive and noninvasive early detection of NSCLC using a quantum dots-based FRET approach

Bai

Mao

et al. 2016

Biosensors and Bioelectronics

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“…222, 225, 226 Indeed, nano-cytology and nano-cytoarchitecture have been proposed as screening targets for field carcinogenesis. 226, 227 It is therefore conceivable that the sophistication of future biosensors will enable accurate assessment of in-vivo real-time changes in the microenvironmental interactome with high resolution (i.e., in-vivo molecular pathology).…”

Section: Advantages and Implications Of The “Etiologic Field Effect” mentioning

confidence: 99%

Etiologic field effect: reappraisal of the field effect concept in cancer predisposition and progression

et al. 2015

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The term “field effect” (also known as field defect, field cancerization, or field carcinogenesis) has been used to describe a field of cellular and molecular alteration, which predisposes to the development of neoplasms within that territory. We explore an expanded, integrative concept, “etiologic field effect”, which asserts that various etiologic factors (the exposome including dietary, lifestyle, environmental, microbial, hormonal, and genetic factors) and their interactions (the interactome) contribute to a tissue microenvironmental milieu that constitutes a “field of susceptibility” to neoplasia initiation, evolution, and progression. Importantly, etiological fields predate the acquisition of molecular aberrations commonly considered to indicate presence of filed effect. Inspired by molecular pathological epidemiology (MPE) research, which examines the influence of etiologic factors on cellular and molecular alterations during disease course, an etiologically-focused approach to field effect can: 1) broaden the horizons of our inquiry into cancer susceptibility and progression at molecular, cellular, and environmental levels, during all stages of tumor evolution; 2) embrace host-environment-tumor interactions (including gene-environment interactions) occurring in the tumor microenvironment; and, 3) help explain intriguing observations, such as shared molecular features between bilateral primary breast carcinomas, and between synchronous colorectal cancers, where similar molecular changes are absent from intervening normal colon. MPE research has identified a number of endogenous and environmental exposures which can influence not only molecular signatures in the genome, epigenome, transcriptome, proteome, metabolome and interactome, but also host immunity and tumor behavior. We anticipate that future technological advances will allow the development of in vivo biosensors capable of detecting and quantifying “etiologic field effect” as abnormal network pathology patterns of cellular and microenvironmental responses to endogenous and exogenous exposures. Through an “etiologic field effect” paradigm, and holistic systems pathology (systems biology) approaches to cancer biology, we can improve personalized prevention and treatment strategies for precision medicine.

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“…Methylated DNA is another promising biomarker in urine or blood that can be used for early detection of cancer and other genetic diseases [276,277]. Quantitation of cellular DNA methylation levels in colon cancer patients has shown to be a promising alternative to colonoscopy for detecting colorectal cancer [278].…”

Section: Dna-and Rna-based Diagnosismentioning

confidence: 99%

Polymeric-Based In Vitro Diagnostic Devices

Cheng

Kuan

Chen

2015

In-Vitro Diagnostic Devices

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Detection and differential diagnosis of colon cancer by a cumulative analysis of promoter methylation

Cited by 70 publications

References 35 publications

A panel of promoter methylation markers for invasive and noninvasive early detection of NSCLC using a quantum dots-based FRET approach

A panel of promoter methylation markers for invasive and noninvasive early detection of NSCLC using a quantum dots-based FRET approach

Etiologic field effect: reappraisal of the field effect concept in cancer predisposition and progression

Polymeric-Based In Vitro Diagnostic Devices

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