Detection and determination of selenoproteins by nuclear techniques

Stone, Susan; Bernasconi, Gabriel D.; Haselberger, N.; Makarewicz, M.; Ogris, R.; Wobrauschek, P.; Zeisler, Rolf

doi:10.1007/bf02917328

Cited by 12 publications

(9 citation statements)

References 11 publications

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“…Stone et al [12] evaluated bench-top microbeam X-ray spectrometry and energy-dispersive XRF analysis for Se detection in glutathione peroxidase after PAGE. Detection limits of Se in the gel matrix were 2.1 ng for the microbeam X-ray system and 30-60 ng using energy-dispersive XRF.…”

Section: X-ray Spectrometrymentioning

confidence: 99%

“…Excellent sensitivity and resolution are achieved with significantly reduced processing time when using modern particle counting detection systems. Cornelis [8], Behne et al [9], and Stone et al [10][11][12] have outlined the applications of radiotracers or instrumental neutron activation analysis with autoradiography for GE-based metalloproteins speciation. Nevertheless, inconvenience due to radiation hazard, radioactive waste disposal and nuclear facilities limit widespread use of the technique.…”

Section: Introductionmentioning

confidence: 99%

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Speciation of protein‐bound trace elements by gel electrophoresis and atomic spectrometry

McLeod

Tomlinson

et al. 2004

Electrophoresis

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The metabolism of trace elements, in particular their binding to proteins in biological systems is of great importance in biochemical, toxicological, and pharmacological studies. As a result there has been a sustained interest over the last two decades in the speciation of protein-bound metals. Various analytical approaches have been employed, combining efficient separation of metalloproteins by liquid chromatography or electrophoresis with high-sensitivity elemental detection. Slab-gel electrophoresis (GE) is a key platform for high-resolution protein separation, and has been combined with autoradiography and various atomic spectrometric techniques for in-gel determination of protein-bound metals. Recently, the combination of GE with state-of-the-art inductively coupled plasma-mass spectrometry (ICP-MS), particularly when linked to laser ablation (LA) for direct gel interrogation, has opened up new opportunities for rapid characterization of metalloproteins. The use of GE and atomic spectrometry for the speciation of protein-bound trace elements is reviewed in this paper. Technical requirements for gel electrophoresis/atomic spectrometric measurement are considered in terms of method compatibilities, detection capability and potential usefulness. The literature is also surveyed to illustrate current status and future trends.

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Section: X-ray Spectrometrymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Speciation of protein‐bound trace elements by gel electrophoresis and atomic spectrometry

McLeod

Tomlinson

et al. 2004

Electrophoresis

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“…Alternatively, selenoproteins separated by PAGE can be Western blotted onto nitrocellulose membranes in which Se is determined by any appropriate method [52]. Some instrumental techniques such as NAA [40,41,51,52] and XRF [54] allow the direct determination of selenium in polyacrylamide gel strips obtained after electrophoresis of selenoproteins. The absolute detection limits of these techniques were 0.1 ng and 2-30 ng, respectively.…”

Section: Polyacrylamide Gel Electrophoresis With Selenium-specific Dementioning

confidence: 99%

Species-selective determination of selenium compounds in biological materials (Technical Report)

Łobiński

Edmonds

Suzuki

et al. 2000

Pure and Applied Chemistry

157

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Republication or reproduction of this report or its storage and/or dissemination by electronic means is permitted without the need for formal IUPAC permission on condition that an acknowledgmentMembership of the Commission during the preparation of this report (1997)(1998)(1999) Species-selective determination of selenium compounds in biological materials (Technical Report)Abstract: There is substantial evidence of the complexity of selenium speciation in living organisms and of the importance of the selective determination of the particular species of this element in order to understand its metabolism and biological significance in clinical chemistry, biology, toxicology, and nutrition. The state-of-the-art of analytical techniques available for this purpose is critically evaluated with particular emphasis on the element-selective detection and identification of the detected selenium compounds. Whereas there are a number of techniques available that are able to detect various selenium species in living organisms selectively, few techniques exist that are able to identify and to characterize the species detected.

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“…different types of chromatography, are adopted for this purpose. Although nuclear analytical techniques (NAT) are thought to be unsuitable for speciation analysis, because they, in principle, are based on nuclear characteristics, rather than on atomic or electronic behavior, in recent years more and more nuclear analysts are endeavoring to use NAT for speciation studies [3,4,5,6,7,8,9,10,11,12,13,14,15]. The main techniques include molecular activation analysis (MAA), proton-induced X-ray emission spectrometry (PIXE), and synchronous radiation-based analytical methods, etc.…”

Section: Introductionmentioning

confidence: 99%

Overview of the methodology of nuclear analytical techniques for speciation studies of trace elements in the biological and environmental sciences

Chai

Mao

et al. 2002

Anal Bioanal Chem

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Recent achievements in speciation studies of trace elements in the biological and environmental sciences by nuclear analytical techniques, mainly molecular activation analysis, position-sensitive spectrometry with a variety of exciting sources, and synchronous radiation-based analytical techniques (although radioisotope or enriched stable isotope-based speciation techniques are also used), particularly in our laboratory, are outlined. In this paper the merits and drawbacks of the nuclear analytical techniques are discussed, as are reagent blanks, contamination, and artifacts.

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Detection and determination of selenoproteins by nuclear techniques

Cited by 12 publications

References 11 publications

Speciation of protein‐bound trace elements by gel electrophoresis and atomic spectrometry

Speciation of protein‐bound trace elements by gel electrophoresis and atomic spectrometry

Species-selective determination of selenium compounds in biological materials (Technical Report)

Overview of the methodology of nuclear analytical techniques for speciation studies of trace elements in the biological and environmental sciences

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