Detection and Determination of Fumonisins B1, B2, and B3 Contaminating Japanese Domestic Wine by Liquid Chromatography Coupled to Tandem Mass Spectrometry (LC–MS/MS)

Nakagawa, Hiroyuki; Hashimoto, Ruiko; Matsuo, Yosuke; Sago, Yuki; Yokoyama, Koji; Takahashi, Hitomi

doi:10.1007/s00284-020-02113-0

Cited by 10 publications

(3 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The risk of FB contamination in PFT could be due to the high temperature and humidity environmental conditions in PFT production, sales, and storage areas (Guangdong and Guangxi in China), providing an ideal condition for the growth of Fusarium sp. [ 43 ]. Notably, the percentage of positive samples for Liupao, Pu-erh, and Fu brick teas packaged in baskets or paper was 21.05%, 22.00%, and 21.43%, respectively.…”

Section: Resultsmentioning

confidence: 99%

B-Type Fumonisins in Post-Fermented Tea: Occurrence and Consumer Dietary Exposure in Guangxi, China

Qiu,

Zhu,

Zhang

et al. 2023

Toxins

View full text Add to dashboard Cite

Post-fermented tea (PFT), a commonly consumed beverage worldwide, is characterized by the rapid growth of its microbial groups and the substantial changes they undergo. Consequently, PFT may contain mycotoxins such as B-type fumonisins (FBs). This study aimed to assess the intake of FBs through the consumption of PFT among consumers in Guangxi, China. A novel quantitative method using high-performance liquid chromatography-mass spectrometry was used to determine the FB concentration in PFT products. Additionally, a PFT consumption survey was conducted using a face-to-face questionnaire, recording their body weight and PFT consumption patterns based on a three-day dietary recall method. Finally, hazard index was calculated to estimate the health risk of FBs from the consumption of PFT products in Guangxi. The results revealed that the occurrence of FBs in PFT was 20% (24/120), with a concentration ranging from 2.14 to 18.28 μg/kg. The results of the survey showed that the average daily consumption of PFT by consumers was 9.19 ± 11.14 g. The deterministic risk assessment revealed that only 0.026% of the provisional maximum tolerable daily intake of FBs was consumed through PFT, indicating that FB contamination in PFT is not a public health risk.

show abstract

Section: Resultsmentioning

confidence: 99%

B-Type Fumonisins in Post-Fermented Tea: Occurrence and Consumer Dietary Exposure in Guangxi, China

Qiu,

Zhu,

Zhang

et al. 2023

Toxins

View full text Add to dashboard Cite

show abstract

“…Mycelia of the cultivated A. tubingensis were removed by filtration with a GF/A glass microfiber filter (pore size 1.6 µm; Whatman, Brentford, UK) at 5 or 8 days (until the growth reached stationary state and metabolites were sufficiently produced) to prepare the culture broth. FBs and ochratoxins were purified from the culture broth as reported previously 23) using immunoaffinity column (IAC) with some modifications. Internal standard solution ( 13 C 34 -FB 1 0.2 mg/L in acetonitrile/water (1:1) or 13 C 20 -OTA 0.2 mg/L in acetonitrile) was individually added at a volume of 0.01 mL with a precision microliter pipette of the "Microman" model M50 (Gilson) to 0.99 mL of each culture broth, to adjust the final concentration of the internal standard ( 13 C 34 -FB 1 or 13 C 20 -OTA) to 2 μg/L.…”

Section: Metabolite Analysis Of Culture Broth Of a Tubingensis Wu-222...mentioning

confidence: 99%

Non-production of mycotoxins by citric acid hyperproducer <i>Aspergillus tubingensis</i> (<i>A. niger</i>) WU-2223L: Evidence for its biosafety based on genome sequence and metabolite analyses

2022

Self Cite

View full text Add to dashboard Cite

The fungal strains belonging to Aspergillus section Nigri such as A. niger and A. tubingensis are industrially important microorganisms. However, some of Aspergillus section Nigri strains are known to produce mycotoxins. In this study, to confirm the non-production of four major mycotoxins (aflatoxins, ochratoxins, fumonisins, and patulin) by citric acid-producing Aspergillus tubingensis (formerly A. niger) WU-2223L, genome sequence analysis and LC-MS/MS analysis were performed. Although short genetic regions homologous to the biosynthetic gene clusters of ochratoxin A and fumonisin B 2 were found in the genome of strain WU-2223L, most of the other parts were missing, indicating that a large portion of the gene clusters were deleted. The genome sequence analysis also revealed that the genomic DNA of the strain WU-2223L possessed no gene homologous to biosynthetic gene clusters for aflatoxins and patulin in addition to ochratoxins and fumonisins. Moreover, the LC-MS/ MS analysis on the broth of strain WU-2223L grown in three typical media revealed that none of the four mycotoxins were detected (below the detection limit). Therefore, the non-production of the four major mycotoxins by the strain WU-2223L was confirmed not only based on the genome sequence analysis, but also on the analysis of metabolites of the strain. Our findings indicate that A. tubingensis WU-2223L is a safe and promising strain that can be used for industrial production of valuable metabolites such as citric acid.

show abstract

“…The traditional methods such as high performance liquid chromatography (HPLC), 8 mass spectrometry (MS), 9 liquid chromatography-MS, 10 gas chromatography-MS, 11 and polymerase chain reactions (PCR) 12 are powerful techniques to detect different kinds of chemical compounds and biological elements. Despite their high sensitivity, accuracy and stability, they are time consuming, laborious and require expensive instruments.…”

Section: Introductionmentioning

confidence: 99%

Gold nanoparticle-based optical nanosensors for food and health safety monitoring: recent advances and future perspectives

et al. 2022

View full text Add to dashboard Cite

show abstract

Detection and Determination of Fumonisins B1, B2, and B3 Contaminating Japanese Domestic Wine by Liquid Chromatography Coupled to Tandem Mass Spectrometry (LC–MS/MS)

Cited by 10 publications

References 20 publications

B-Type Fumonisins in Post-Fermented Tea: Occurrence and Consumer Dietary Exposure in Guangxi, China

B-Type Fumonisins in Post-Fermented Tea: Occurrence and Consumer Dietary Exposure in Guangxi, China

Non-production of mycotoxins by citric acid hyperproducer <i>Aspergillus tubingensis</i> (<i>A. niger</i>) WU-2223L: Evidence for its biosafety based on genome sequence and metabolite analyses

Gold nanoparticle-based optical nanosensors for food and health safety monitoring: recent advances and future perspectives

Contact Info

Product

Resources

About