Detection and Classification of Soft Rot Erwinia of Vegetables in Thailand by Dna Polymerase Chain Reaction

Studies on pectinase activities of isolates of Erwinia carotovora and Rhizopus sp. causing soft rot in cabbage (Brassica oleracea var capitata L.)

“…Results clearly indicate that there is considerable variation among isolates of E. carotovora with respect to macerating ability and it is also evident that the soft rot caused by bacteria is more rapid than that caused by the fungal species used in the study. Studies depicting the variability among soft rot causing isolates of E. carotovora with respect to their disease inducing ability are in conformity with those of Phokum et al (2006) who also reported that Erwinia isolate from carrot was most severe when compared with isolates of ten different varieties of vegetables. Smith and Bartz (1990) also reported significant differences among the strains of E. carotovora for their aggressiveness on tubers and fruits of some vegetables.…”

“…The results on enzyme activities by all the methods are similar and hence confirming their accuracy with each other and that of maceration test with only the exception that assay for pectin methyl esterase (PME) cannot be carried out with viscometric assay as pectin methyl esterase (PME) cannot reduce viscosity. Phokum et al (2006) also reported variations in pectin lyase and polygalacturonases (PG) by spectrometer and found that Erwinia isolate from carrot was more severe as it produced higher amount of enzymes and macerated more tissue than the ten other isolates of pathogen. El-Shaieb and Milibari (1995) also reported variation among isolates of E. caratovora with respect to their enzyme activities of PME and PG (polygalacturonase).…”

“…There have been several reports in the past suggesting pathogenic superiority of strains E. carotovora associated with the production of greater quantity of pecteolytic enzymes (Gregg, 1952;El-Shaieb and Milibari, 1995). Certain other reports of high level of aggressiveness associated with strains of E. carotovora have been associated with their abilities to secrete higher amounts of macerating enzymes (Phokum et al, 2006;Smith and Bartz, 1990). Present investigations were carried with the objectives to detect differences in aggressiveness or macerating ability of different soft rot causing isolates of E. carotovora and differences in their abilities to produce pectic enzymes like Pectin methylestraces (PME) and chain splitting enzymes that is, hydrolases and lyases in cell free culture filtrates of different Erwinia isolates.…”

Antagonistic bioagent mechanisms of controlling potato soft rot

“…In addition, bacterial soft rot has been reported to affect most cruciferous and other crops, such as cabbages, cauliflowers, carrots, turnips, radishes, rape, tomatoes, lettuces, onions, beetroots, spinach, mangoes, celery, sunflowers, chicory, coriander, arracacha, cucumbers, giant pumpkins (Takimoto 1931;Smith 1944;Chakravarti & Rangarajan 1966;Arsenijevic 1970;Wimalajeewa 1976;Guevara et al 1980;Romeiro et al 1988;Gallois et al 1992;Schuerger & Batzer 1993;Phokim et al 2006). Soft rot is responsible for about 30% of losses in potato tubers (Farrar et al 2009).…”

PCR-identification of bacteria belonging to the genus Pectobacterium – agents of cucumber soft rot and wilting in Ukraine

“…Зважаючи на це, метою наших досліджень була видова ідентифікація ізольованих нами Pectobacterium sp., колекційних «Erwinia toxica» штамів і типових представників окремих видів родів Pectobacterium і Dickeya за допомогою ПЛР. [5,8,9]. Деякі науковці, спираючись на результати власних досліджень, позиціонують даний підхід як спосіб ідентифікації виключно представників виду Pectobacterium carotovorum.…”