Detection and characterization of pathogenic Pseudomonas aeruginosa from bovine subclinical mastitis in West Bengal, India

Banerjee, Sandip; Batabyal, K.; Joardar, Siddhartha Narayan; Isore, D.P.; Dey, Samir; Samanta, Indranil; Samanta, Tanmoy; Murmu, S.

doi:10.14202/vetworld.2017.738-742

Cited by 38 publications

(31 citation statements)

References 8 publications

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“…P. aeruginosa is one of the more common conditional pathogens in the clinic and is also a common bacterial infection in wounds. A literature search showed that P. aeruginosa has been isolated from mastitis tissues of bovines and caprines (Banerjee et al, 2017;Scaccabarozzi et al, 2015), but there are few reports of this pathogen infection in human mastitis. P. stutzeri is a Gramnegative, rod-shaped, aerobic, catalase-and oxidase-positive bacterium that is ubiquitously present in the environment.…”

Section: Discussionmentioning

confidence: 99%

Pathogens in patients with granulomatous lobular mastitis

Wang

et al. 2019

International Journal of Infectious Diseases

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Objective: Granulomatous lobular mastitis (GLM) is a rare inflammatory disease of the breast that clinically mimics breast cancer. However, its etiology is not completely defined. The purpose of this study was to systematically study the bacteriology of GLM using advanced detection technology. Methods: Paraffin-embedded tissue from patients with GLM was collected. DNA was extracted from the samples and analyzed using next-generation sequencing (NGS) technology, and the data were processed using bioinformatics analyses. Results: A total of 40 patients were recruited into the study. A bioinformatics analysis revealed that a total of 17 genera or 19 species of pathogens were present in 39 of the GLM patients (97.5%). These included bacteria, fungi, and Mycobacterium tuberculosis complex group. Bacteria were found in 39 of the patient cases, while fungi were present in five. Only one case tested positive for M. tuberculosis complex. In addition, a single genus of pathogen was found in nine patients (23.1%), whereas 30 patients (76.9%) tested positive for multiple pathogens. Conclusions: This study profiled the microbiota of patients with GLM using NGS technology, which provides more useful information for establishing patient treatment plans.

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Section: Discussionmentioning

confidence: 99%

Pathogens in patients with granulomatous lobular mastitis

Wang

et al. 2019

International Journal of Infectious Diseases

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“…It is an effective antibiotic agent against bovine mastitis which is primarily caused by bacteria (Saidi et al, 2019) (Ruegg, 2017). In India, mastitis causes major annual economic losses (Banerjee et al, 2017). Antibiotics are highly soluble in water and are the least usable biologically.…”

Section: Introductionmentioning

confidence: 99%

Genotoxic impact of emerging contaminant amoxicillin residue on zebra fish (Danio rerio) embryos

Chowdhury

Mandal

Mondal

2020

Heliyon

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The widest-spectrum, most-consumed β-lactam antibiotic amoxicillin (AMX) is used to treat bovine mastitis that is caused primarily by many bacteria. Excessive use of antibiotics can lead to established residual contamination of the milk even after pasteurization. The amount of antibiotic residue above Maximum Residue Limit (MRL) has a negative impact on both public health and the environment. Therefore, the objective of this study is to determine the concentration of amoxicillin residue (AMXR) in raw and pasteurized milk samples of cow suffered from mastitis, by the standard methods of HPLC compared to pure AMX drug and effect of the said residue on the developmental toxicity and genotoxicity of zebra fish at 72 hpf and 48 hpf embryo, respectively. Results obtained by HPLC showed that AMXR exhibits 574.89 and 250.75 times higher concentration in the raw and pasteurized milk than MRL in compare to pure AMX drug. This current study showed that AMXR decreased the body length and yolk sac region, while the pure AMX drug-treated group showed increased height and length of the yolk sac and shorter body length relative to the other groups. The Comet Assay measured the DNA damage caused by AMXR. The group where AMXR were applied showed the highest percentage of tail DNA and tail moment relative to other groups. So, here AMXR is considered as the genotoxic contaminant that is emerging and affect on public health.

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“…A variety of virulence factors contribute to the pathogenicity of P.aeruginosa and are targeted by PCR for its detection such as oprL gene and exoS gene (6), gyrB gene (2,7), ecfX genes (8), Quorum sensing gene (9), gene encoded to phospholipase (plcH), rhamnolipid AB (rhlAB), alkaline protease (aprA) and elastase (lasB) (10) beside exotoxin A (ETA gene) (11,12). ETA gene is species specific and conserved for P.aeruginosa species and is not present in other species of Psuedomonas genus.…”

Section: Introductionmentioning

confidence: 99%

Detection of Pseudomonas aeruginosa in Clinical Samples Using PCR Targeting ETA and gyrB Genes

Journal¹

2018

Baghdad Sci.J

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Pseudomonas aeruginosa has variety of virulence factors that contribute to its pathogenicity. Therefore, rapid detection with high accuracy and specificity is very important in the control of this pathogenic bacterium. To evaluate the accuracy and specificity of Polymerase Chain Reaction (PCR) assay, ETA and gyrB genes were targeted to detect pathogenic strains of P. aeruginosa. Seventy swab samples were taken from patients with infected wounds and burns in two hospitals in Erbil and Koya cities in Iraq. The isolates were traditionally identified using phenotypic methods, and DNA was extracted from the positive samples, to apply PCR using the species specific primers targeting ETA, the gene encoding for exotoxin A, and gyrB gene. The results of this study indicate that 100% of P. aeruginosa isolates harbored the gyrB gene, whereas 74% of these isolates harbored ETA gene. However, the specificity of PCR for detection of P. aeruginosa based on the both genes was 100%, since no amplified product obtained using DNA extracted from other bacterial species. Hence by considering the importance of rapid detection of this bacterium due to the presence of problems in biochemical methods, PCR targeting multiple virulence genes is suggested in identification of pathogenic strains of P. aeruginosa isolated from some infections which should speed diagnosis of an antimicrobial therapy.

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Detection and characterization of pathogenic Pseudomonas aeruginosa from bovine subclinical mastitis in West Bengal, India

Cited by 38 publications

References 8 publications

Pathogens in patients with granulomatous lobular mastitis

Pathogens in patients with granulomatous lobular mastitis

Genotoxic impact of emerging contaminant amoxicillin residue on zebra fish (Danio rerio) embryos

Detection of Pseudomonas aeruginosa in Clinical Samples Using PCR Targeting ETA and gyrB Genes

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