Detection and Characterization of Chickpea Chlorotic Stunt Virus in Syria

Abstract

Field surveys were conducted in Syria during the 2005 ⁄ 2006 and 2006 ⁄ 2007 growing seasons to identify viruses which affect cool-season food legumes, volunteer crops, and weeds with yellowing, reddening and ⁄ or stunting symptoms. Serological tests (tissue blot immunoassay) showed that Faba bean necrotic yellows virus and luteoviruses including, Beet western yellows virus, Bean leafroll virus and Soybean dwarf virus were the main viruses identified. In addition, a large number of samples reacted positively with the broad spectrum legume luteovirus (5G4) monoclonal antibody but not with any other specific luteovirus monoclonal antibodies suggesting the occurrence of new luteovirus species ⁄ strains. RT-PCR assay of 27 of such samples revealed the presence of Chickpea chlorotic stunt virus (CpCSV; genus Polerovirus, family: Luteoviridae). The coat protein nucleotide sequence analysis of the Syrian CpCSV isolate from chickpea showed a high homology (98%) to that of the Ethiopian CpCSV isolate. However, there were some differences in host range and aphid transmission characteristics between the Syrian and Ethiopian isolates. This is the first report of CpCSV naturally affecting eight legume species (Cicer arietinum L., Lens culinaris Medik., Pisum sativum L., Vicia faba L., Vicia sativa L., Vicia ervilia L. Willd., Vicia narbonensis L. and Medicago sp.) and four wild non-legume plant species [Apium sp. (Apiaceae), Euphorbia sp. (Euphorbiaceae), Physalis longifolia Nutt. (Solanaceae) and Sinapis arvensis L. (Brassicaceae)] in Syria.

Genetic diversity and recombination between turnip yellows virus strains in Australia

“…TBIAs were processed as described by Filardo and Sharman [23]. The primary antibody used to detect poleroviruses, including TuYV, was the broad-spectrum luteovirid monoclonal antibody BLRV-5G4 [2,34]. [44], as per manufacturer's instructions.…”

Viruses infecting chickpea and lentil crops in Azerbaijan

“…The most important viruses causing yellowing and stunting symptoms reported to infect chickpea and lentil in Central and West Asia and North Africa (CWANA) region are: Bean leafroll virus (BLRV, genus Luteovirus, family Luteoviridae), Beet western yellows virus (BWYV, genus Polerovirus, family Luteoviridae), Chickpea chlorotic dwarf virus (CpCDV, genus Mastrevirus, family Geminiviridae), and Faba bean necrotic yellows virus (FBNYV, genus Nanovirus, family Nanoviridae). Recently, Chickpea chlorotic stunt virus (CpCSV, genus Polerovirus, family Luteoviridae) has been characterized and reported to affect chickpea and other legume crops in Ethiopia, Eritrea, Syria, Iran, Morocco, Egypt and Sudan (Abraham et al 2006(Abraham et al , 2009Kumari et al 2008;Asaad et al 2009;Bananej et al 2010). CpCSV may have been identified in earlier surveys in CWANA region as BWYV or unidentified Luteoviridae species, due to the lack of specific serological diagnostic tools (Horn et al 1995b;Makkouk et al 2003).…”

Breeding for biotic stress resistance in chickpea: progress and prospects

“…Significant yield losses due to CpCDV, of 60-100 %, have been reported in Sudan and India (Horn et al 1995). CpCSV (genus Polerovirus, family Luteoviridae) is a recently described virus that infects cool-season food legumes (Abraham et al 2006(Abraham et al , 2009Asaad et al 2009) and is a phloem-limited virus that is present in very low concentration and transmitted by aphids (Aphis craccivora Koch. and Aphis pisum Harris) in a persistent manner (Abraham et al 2006;Asaad et al 2009).…”

“…CpCSV (genus Polerovirus, family Luteoviridae) is a recently described virus that infects cool-season food legumes (Abraham et al 2006(Abraham et al , 2009Asaad et al 2009) and is a phloem-limited virus that is present in very low concentration and transmitted by aphids (Aphis craccivora Koch. and Aphis pisum Harris) in a persistent manner (Abraham et al 2006;Asaad et al 2009). CpCSV symptoms cannot be distinguished from those caused by other yellowing viruses like BLRV or BWYV.…”