Detection and characterization of human rotavirus in hospitalized patients in the cities of Ponta Grossa, Londrina and Assai - Pr, Brazil

Nozawa, Carlos; Kerntopf, Gustavo Franthesco; Czernisz, Érika da Silva; Albuquerque, Daniele Menezes; Romanin, Priscila; Freitas, José Felipe Eliseu; Santos, Norma; Benati, Fabrício José; Pietruchinski, Eduardo; Linhares, Rosa Elisa Carvalho

doi:10.1016/s1413-8670(10)70111-2

Cited by 3 publications

(2 citation statements)

References 35 publications

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“…However, in the last few years Argentina, Chile and Brazil have reported new genotypes that have apparently spread in Latin America. Knowledge of their distribution, including detection of emerging genotypes, is crucial for rotavirus vaccination programs 8,9,10 .…”

Section: Introductionmentioning

confidence: 99%

Diarrhoea caused by rotavirus in a regional Peruvian hospital: determination of circulating genotypes

Espejo

Peralta

Pacheres

et al. 2014

Transactions of the Royal Society of Tropical Medicine and Hygi

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Section: Introductionmentioning

confidence: 99%

Diarrhoea caused by rotavirus in a regional Peruvian hospital: determination of circulating genotypes

Espejo

Peralta

Pacheres

et al. 2014

Transactions of the Royal Society of Tropical Medicine and Hygi

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“…Many studies have focused on molecular characterisation of the NSP4 gene of HuRVA collected from different regions of Brazil ( Mascarenhas et al 2006 , Araújo et al 2007b, Tavares et al 2008 , Nozawa et al 2010 , Fumian et al 2011 , Gómez et al 2011 ); however, no studies have examined HuRVA strains collected in the state of São Paulo (SP). The aim of this study was to characterise the E genotypes and NSP4 aa sequences of 41 HuRVA strains from SP that were previously identified as G2P[4].…”

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confidence: 99%

Molecular characterisation of the NSP4 gene of group A human rotavirus G2P[4] strains circulating in São Paulo, Brazil, from 1994 and 2006 to 2010

Bertól

Fregolente

Caruzo

et al. 2015

Mem. Inst. Oswaldo Cruz

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Group A human rotaviruses (HuRVA) are causative agents of acute gastroenteritis. Six viral structural proteins (VPs) and six nonstructural proteins (NSPs) are produced in RV-infected cells. NSP4 is a diarrhoea-inducing viral enterotoxin and NSP4 gene analysis revealed at least 15 (E1-E15) genotypes. This study analysed the NSP4 genetic diversity of HuRVA G2P[4] strains collected in the state of São Paulo (SP) from 1994 and 2006-2010 using reverse transcription-polymerase chain reaction, sequencing and phylogenetic analysis. Forty (97.6%) G2P[4] strains displayed genotype E2; one strain (2.4%) displayed genotype E1. These results are consistent with the proposed linkage between VP4/VP7 (G2P[4]) and the NSP4 (E2) genotype of HuRVA. NSP4 phylogenetic analysis showed distinct clusters, with grouping of most strains by their genotype and collection year, and most strains from SP were clustered together with strains from other Brazilian states. A deduced amino acid sequence alignment for E2 showed many variations in the C-terminal region, including the VP4-binding domain. Considering the ability of NSP4 to generate host immunity, monitoring NSP4 variations, along with those in the VP4 or VP7 protein, is important for evaluating the circulation and pathogenesis of RV. Finally, the presence of one G2P[4]E1 strain reinforces the idea that new genotype combinations emerge through reassortment and independent segregation.

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Review of global rotavirus strain prevalence data from six years post vaccine licensure surveillance: Is there evidence of strain selection from vaccine pressure?

Dóró

Békési

Martella

et al. 2014

Infection, Genetics and Evolution

195

217

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Comprehensive reviews of pre licensure rotavirus strain prevalence data indicated the global importance of six rotavirus genotypes, G1P[8], G2P[4], G3P[8], G4P[8], G9P[8] and G12P[8]. Since 2006, two vaccines, the monovalent Rotarix (RV1) and the pentavalent RotaTeq (RV5) have been available in over 100 countries worldwide. Of these, 60 countries have already introduced either RV1 or RV5 in their national immunization programs. Post licensure vaccine effectiveness is closely monitored worldwide. This review aimed at describing the global changes in rotavirus strain prevalence over time. The genotype distribution of the nearly 47,000 strains that were characterized during 2007-2012 showed similar picture to that seen in the preceding period. An intriguing finding was the transient predominance of heterotypic strains, mainly in countries using RV1. Unusual and novel antigen combinations continue to emerge, including some causing local outbreaks, even in vaccinated populations. In addition, vaccine strains have been found in both vaccinated infants and their contacts and there is evidence for genetic interaction between vaccine and wild-type strains. In conclusion, the post-vaccine introduction strain prevalence data do not show any consistent pattern indicative of selection pressure resulting from vaccine use, although the increased detection rate of heterotypic G2P[4] strains in some countries following RV1 vaccination is unusual and this issue requires further monitoring.

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Detection and characterization of human rotavirus in hospitalized patients in the cities of Ponta Grossa, Londrina and Assai - Pr, Brazil

Abstract: The data showed similar electropherotypes and genotypes G, P and NSP4 of the inland wild circulating strains of RV.

Cited by 3 publications

References 35 publications

Diarrhoea caused by rotavirus in a regional Peruvian hospital: determination of circulating genotypes

Diarrhoea caused by rotavirus in a regional Peruvian hospital: determination of circulating genotypes

Molecular characterisation of the NSP4 gene of group A human rotavirus G2P[4] strains circulating in São Paulo, Brazil, from 1994 and 2006 to 2010

Review of global rotavirus strain prevalence data from six years post vaccine licensure surveillance: Is there evidence of strain selection from vaccine pressure?

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