Detection and characterization of defective interfering RNAs associated with the cocksfoot mottle sobemovirus

Mäkinen, Kristiina; Generozov, Edward V.; N, Arshava; Kaloshin, A. A.; Morozov, Sergey Y.; Завриев, С. К.

doi:10.1007/bf02759654

Cited by 8 publications

(8 citation statements)

References 36 publications

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“…Five DI RNAs of CfMV have been found, corresponding to 35-40 nucleotides of the 5'-proximal end of genomic RNA linked with 850-950 nucleotides of the 3' terminus (Mäkinen et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

An attempt to identify recombinants between two sobemoviruses in doubly infected oat plants

Meier

Truve

2006

Environ. Biosafety Res.

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Recombination in RNA viruses is considered to play a major role as a driving force in virus variability to counterbalance loss in fitness that can be due to the accumulation of detrimental mutations. Studies on mixed infections are pertinent for understanding the role of recombination in virus evolution. They also provide important baseline information for studying the biosafety of plants expressing viral sequences. To investigate the possibility of RNA recombination occurrence between two sobemoviruses under little or no selection pressure, we co-infected test plants with Cocksfoot mottle virus (CfMV) and Ryegrass mottle virus (RGMoV). CfMV and RGMoV were selected because of their overlapping host range and geographical distribution. First, symptom development of both viruses in barley (Hordeum vulgare) and oat (Avena sativa) was examined. Both viruses generated quite strong infection symptoms in oat, but synergism was not detected. RGMoV was lethal for barley, whereas CfMV infection in barley was nearly symptomless. RT-PCR analysis revealed 100% infection with both viruses in oat but not in barley. Therefore, an RNA recombination study of CfMV and RGMoV was performed in oat. 105 plants were co-inoculated with both viruses and putative recombinational hot spot regions were screened for recombination events by RT-PCR analysis at a sensitivity level down to 0.1-100 pg of viral genomic RNA. No recombination events between the two sobemoviruses were detected.

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“…Five DI RNAs of CfMV have been found, corresponding to 35-40 nucleotides of the 5'-proximal end of genomic RNA linked with 850-950 nucleotides of the 3' terminus (Mäkinen et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

An attempt to identify recombinants between two sobemoviruses in doubly infected oat plants

Meier

Truve

2006

Environ. Biosafety Res.

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“…The sgRNA has been detected both in sobemovirus particles and in infected tissues [ 65 , 75 , 78 , 79 , 80 , 81 , 82 ]. CfMV has also been reported to encapsulate at least five different viral defective interfering RNA molecules (DI RNA) corresponding to 35–40 nt of the 5′ terminus linked to 850–950 nt of the 3′ terminus [ 83 ]. Generally, the existence of DI RNA molecules is considered as a proof of the replicase-driven template switching mechanism needed for the creation of recombinant RNA molecules [ 84 ].…”

Section: Genomic Organizationmentioning

confidence: 99%

Overview on Sobemoviruses and a Proposal for the Creation of the Family Sobemoviridae

Sõmera

Sarmiento

Truve

2015

Viruses

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The genus Sobemovirus, unassigned to any family, consists of viruses with single-stranded plus-oriented single-component RNA genomes and small icosahedral particles. Currently, 14 species within the genus have been recognized by the International Committee on Taxonomy of Viruses (ICTV) but several new species are to be recognized in the near future. Sobemovirus genomes are compact with a conserved structure of open reading frames and with short untranslated regions. Several sobemoviruses are important pathogens. Moreover, over the last decade sobemoviruses have become important model systems to study plant virus evolution. In the current review we give an overview of the structure and expression of sobemovirus genomes, processing and functions of individual proteins, particle structure, pathology and phylogenesis of sobemoviruses as well as of satellite RNAs present together with these viruses. Based on a phylogenetic analysis we propose that a new family Sobemoviridae should be recognized including the genera Sobemovirus and Polemovirus. Finally, we outline the future perspectives and needs for the research focusing on sobemoviruses.

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“…(Graves et al, 1996, review) Tospovirus TSWV L RNA DI RNA Encodes polymerase protein (Nagata et al, 2000) M RNA D Virus Loss of viral envelope and probable loss of vector transmission (Nagata et al, 2000) PBNV L RNA D Virus Gowda et al (1998) Rhabdoviridae SYNV Ismail and Milner (1988) Bromoviridae AMV RNA3 D RNA Graves et al (1996) (Graves et al, 1996, review) RNAs 3 and 4 D Virus Loss of vector transmission and ability to infect roots (Graves et al, 1996, review) Pecluvirus PCV RNA2 D Virus Loss of vector transmission (Graves et al, 1996, review) Benyvirus BNYVV RNA2 D Virus Loss of vector transmission (Graves et al, 1996, review) Sobemovirus CfMV Central region DI RNA Makinen et al (2000) Group 2 Analysis of these plants revealed that the levels of p19 (suppressor of RNA silencing), the p22 (movement protein), and sg mRNA2 were reduced much more than the level of the genomic RNA replication proteins.…”

Section: B Modulation Of the Functions Of Viral Factorsmentioning

confidence: 99%

Replication of Plant Viruses

Hull

2014

Plant Virology

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Detection and characterization of defective interfering RNAs associated with the cocksfoot mottle sobemovirus

Cited by 8 publications

References 36 publications

An attempt to identify recombinants between two sobemoviruses in doubly infected oat plants

An attempt to identify recombinants between two sobemoviruses in doubly infected oat plants

Overview on Sobemoviruses and a Proposal for the Creation of the Family Sobemoviridae

Replication of Plant Viruses

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