Detection and characterization of anionic polypeptidic fraction binding sites in rat liver plasma membranes and cultured hepatocytes

Martigne, Monique; Melin, B; Mahlberg, Florence H.; Domingo, N.; Chanussot, F.; Lafont, Huguette; Hauton, J. C.

doi:10.1016/0005-2736(89)90254-x

Cited by 12 publications

(5 citation statements)

References 20 publications

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“…Thus, the above data suggest that the effect of PC on gallstone formation is not related to consistent changes in biliary lipid or bile acid secretion. APF was originally described as part of a vesicular bile lipoprotein complex (d ϭ 1.030 g/ml) (17). AFP is present in higher concentrations (0.4 g/l) in biliary vesicles compared with the biliary micellar phase (0.3 g/l) (the APF-PL ratio was reported to average 219 in vesicles vs. 30 in micelles) (48).…”

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confidence: 99%

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Phosphatidylcholine-enriched diet prevents gallstone formation in mice susceptible to cholelithiasis

Kasbo

Tuchweber

Perwaiz

et al. 2003

Journal of Lipid Research

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Cholesterol gallstones affect approximately 10-15% of the adult population in North America. Phosphatidylcholine (PC) is considered to be the main cholesterol solubilizer in bile. This study examined the effect of a PCenriched diet on gallstone incidence in mice susceptible to cholelithiasis. The result obtained showed that the feeding of a lithogenic (LG) diet for 4 weeks or 8 weeks resulted in cholesterol gallstone incidences of 47% and 89%, respectively. These gallstone incidences were either reduced or prevented when the LG diet was enriched with 2% or 6% PC, respectively. The cholesterol saturation index (CSI) was reduced only in mice fed with LG ؉ 6% PC diet as compared with mice fed the LG diet alone. However, in all groups, the CSI was significantly higher than in mice fed Purina chow diet. The biliary anionic polypeptide fraction (APF) was significantly increased in mice fed the LG ؉ 2% PC diet and was reduced in those fed with LG ؉ 6% PC diet. In conclusion, prevention or delay of gallstone formation was not due to a consistent effect on biliary lipid composition, suggesting a direct effect of PC on cholesterol solubilization and/or the effect of an additional nonlipid biliary component such as APF.

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“…It has been demonstrated that APF is involved in the uptake of unesterified cholesterol (15) and stimulates cholesterol and PL transport to the bile (16,17). It was also recently demonstrated that APF plays an important role in controlling the onset and rates of precipitation of calcium salts, bile pigments, and cholesterol in gallstone formation (18,19).…”

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confidence: 99%

Phosphatidylcholine-enriched diet prevents gallstone formation in mice susceptible to cholelithiasis

Kasbo

Tuchweber

Perwaiz

et al. 2003

Journal of Lipid Research

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“…Hence even after dilution in the intestinal lumen there is a relative high content of APF/CBP in the intestine. The protein itself has a low capacity for cholesterol but is has been shown to be able to mediate efflux towards bile acid micelles (7,20). In vivo experiments in an animal model will have to be carried out to validate this functional role for APF/CBP.…”

Section: Discussionmentioning

confidence: 99%

Biliary Anionic Peptide Fraction/Calcium Binding Protein Inhibits Apolipoprotein A-I-Mediated Cholesterol Efflux from Cultured Cells

Wijland

Waart

Groen

2001

Biochemical and Biophysical Research Communications

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“…Thus, it seemed of interest to compare the effects of crilvastatin and a reference cholesterol lowering drug, simvastatin, on the LDLcholesterol metabolism in bile salts of cultured Hep G2 cells or human hepatocytes in the presence of ursodeoxycholate. The cholesterol lowering effect of the two drugs was also evaluated by comparing the levels of the three main cholesterol-carrier apoproteins, apo B100, apo Al and APF, a new 7.5 kDa hydrophobic biliary protein (Martigne et al, 1989;Domingo et al, 1990). APF has also been shown to be a minor apoprotein component of plasmatic nascent HDL, HDL2 and HDL3, and to have a partial sequence homology with apo Al (Domingo et al., 1992).…”

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confidence: 99%

Differences in hypolipidaemic effects of two statins on Hep G2 cells or human hepatocytes in primary culture

Clerc

Sbarra

Domingo

et al. 1996

British J Pharmacology

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. The objective of this study was to compare in cultured human hepatocytes or Hep G2 cells, changes in the fate of unesterified low density lipoprotein (LDL)‐cholesterol induced by crilvastatin, a new cholesterol lowering drug and a reference statin, simvastatin. . The experiments were carried out for 20 h, each well contained 4.2 × 105/cm2 Hep G2 cells or 0.5 × 105/cm2 human hepatocytes, 130 μm ursodeoxycholate, 0.68 μCi or 1.59 μCi unesterified human [14C]‐LDL‐cholesterol, crilvastatin or simvastatin at 0 or 50 μm (both cell types) or 300 μm (Hep‐G2 cells). Incubation with the two drugs resulted in increased amounts of unesterified [14C]‐LDL‐cholesterol taken by the two cell types, compared to control. . Crilvastatin 50 μm led to significantly higher quantities of [14C]‐glyco‐ and [14C]‐tauro‐conjugated bile salts, compared to simvastatin. Statins reduced the apo B100 level secreted by the two cell types (simvastatin) or human hepatocytes (crilvastatin). Crilvastatin enhanced both the level of apo A1 secreted by the Hep G2 cells and the level of APF, a high density lipoprotein (HDL) and biliary apoprotein. . Crilvastatin not only acts by stimulating LDL‐cholesterol uptake by hepatocytes, but also by enhancing the catabolism of LDL‐cholesterol in bile salts and probably by stimulating HDL and/or bile component secretion. Such a mechanism was not previously described for HMG CoA reductase inhibitors. Our results on APF show that this apoprotein could be considered also as an indicator of changes in bile and/or HDL compartments. . The human hepatocyte model appeared to be a suitable and relevant model in the pharmacological***metabolic experiments carried out in this study. It led to more consistent data than those obtained with Hep G2 cells.

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Detection and characterization of anionic polypeptidic fraction binding sites in rat liver plasma membranes and cultured hepatocytes

Cited by 12 publications

References 20 publications

Phosphatidylcholine-enriched diet prevents gallstone formation in mice susceptible to cholelithiasis

Phosphatidylcholine-enriched diet prevents gallstone formation in mice susceptible to cholelithiasis

Biliary Anionic Peptide Fraction/Calcium Binding Protein Inhibits Apolipoprotein A-I-Mediated Cholesterol Efflux from Cultured Cells

Differences in hypolipidaemic effects of two statins on Hep G2 cells or human hepatocytes in primary culture

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