Detection and Characterization of a Thermophilic Biotin Biosynthetic Enzyme, 7-Keto-8-aminopelargonic Acid Synthase, from Various Thermophiles

Kubota, Takaaki; Izumi, Yoshikazu

doi:10.1271/bbb.110807

Cited by 4 publications

(3 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…BioF, one of the CKGs, is an enzyme that catalyzes the first step in the biotin synthesis pathway. Its structure and function have been well studied in microorganisms such as E. coli , B. subtilis , and B. sphaericus [ 68 , 69 ], but not in S. Typhimurium [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

Analysis of In Vivo Transcriptome of Intracellular Bacterial Pathogen Salmonella enterica serovar Typhmurium Isolated from Mouse Spleen

Sun

Song

et al. 2021

Pathogens

View full text Add to dashboard Cite

Salmonella enterica serovar Typhimurium (S. Typhimurium) is an important intracellular pathogen that poses a health threat to humans. This study tries to clarify the mechanism of Salmonella survival and reproduction in the host. In this study, high-throughput sequencing analysis was performed on RNA extracted from the strains isolated from infected mouse spleens and an S. Typhimurium reference strain (ATCC 14028) based on the BGISEQ-500 platform. A total of 1340 significant differentially expressed genes (DEGs) were screened. Functional annotation revealed DEGs associated with regulation, metabolism, transport and binding, pathogenesis, and motility. Through data mining and literature retrieval, 26 of the 58 upregulated DEGs (FPKM > 10) were not reported to be related to the adaptation to intracellular survival and were classified as candidate key genes (CKGs) for survival and proliferation in vivo. Our data contribute to our understanding of the mechanisms used by Salmonella to regulate virulence gene expression whilst replicating inside mammalian cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Analysis of In Vivo Transcriptome of Intracellular Bacterial Pathogen Salmonella enterica serovar Typhmurium Isolated from Mouse Spleen

Sun

Song

et al. 2021

Pathogens

View full text Add to dashboard Cite

show abstract

“…BioF is an enzyme that catalyzes the first step in the biotin synthesis pathway. Its structure and function have been well studied in microorganisms such as E. coli, B. subtilis, and B. sphaericus [36,37], but not in S. typhimurium [35].…”

Section: Spia Of S Typhimurium Is Critical For the Virulence Of Hostmentioning

confidence: 99%

Screening and Identification of Key Genes for the Survival and Multiplication of Salmonella typhimurium in the Host

Sun

Song

Liu

et al. 2019

Preprint

View full text Add to dashboard Cite

BackgroundSalmonella typhimurium is an important intracellular pathogen that poses a health threat to humans. The key to studying the pathogenesis of Salmonella is to clarify the mechanisms responsible for its survival and reproduction in macrophages. In this study, RNA was extracted from S. typhimurium reference strain (ATCC 14028) and S. typhimurium isolated from the spleen of infected mice for RNA high-throughput sequencing analysis, based on the BGISEQ-500 platform.ResultsA total of 1,340 significant differentially expressed genes (DEGs) were screened through quantitative gene analysis and various analyses based on gene expression. Of these, 16 genes were randomly selected for fluorescence quantitative PCR verification. Two pairs of primers, 16S and pyridoxol 5ʹ-phosphate synthase (pdxJ), were used as internal references. The coincidence rate was determined to be 93.75%, which was consistent with the RNA-seq data, proving the reliability of the RNA-seq data. Functional annotation revealed DEGs associated with regulation, metabolism, transport and binding, pathogenesis, and motility. Through data mining and literature retrieval, 26 of the 58 upregulated DEGs (FPKM >10) were not reported to be related to the adaptation to intracellular survival, and were classified as candidate key genes (CKGs) for survival and proliferation in vivo. Among the CKGs, five were biotin synthetic bio family proteins. BioF is one of the first enzymes in the protein synthesis pathway. To evaluate the potential role of bioF in survival and proliferation, bioF mutants of Salmonella were constructed, and the virulence/attenuation was evaluated in vivo. Through the infection of BALB/c mice, bioF deficiency was found to significantly reduce the bacterial load and the fatality rate of mice. ConclusionsOur results indicated that the bioF gene plays an important role in the survival and proliferation of S. typhimurium in vivo. These data contribute to our understanding of the mechanisms used by Salmonella to regulate virulence gene expression whilst replicating inside mammalian cells.

show abstract

“…A plausible explanation is that BioI is only present in a limited number of microorganisms, and its pathway may have been obstructed during long-term evolution. The BioF protein is also identified in various thermophiles with higher thermostability, facilitating its application for microbial biotin production at elevated temperatures [ 54 ].…”

Section: Introductionmentioning

confidence: 99%

Advances and prospects in microbial production of biotin

Ma,

Du,

Fang

et al. 2024

Microb Cell Fact

View full text Add to dashboard Cite

Biotin, serving as a coenzyme in carboxylation reactions, is a vital nutrient crucial for the natural growth, development, and overall well-being of both humans and animals. Consequently, biotin is widely utilized in various industries, including feed, food, and pharmaceuticals. Despite its potential advantages, the chemical synthesis of biotin for commercial production encounters environmental and safety challenges. The burgeoning field of synthetic biology now allows for the creation of microbial cell factories producing bio-based products, offering a cost-effective alternative to chemical synthesis for biotin production. This review outlines the pathway and regulatory mechanism involved in biotin biosynthesis. Then, the strategies to enhance biotin production through both traditional chemical mutagenesis and advanced metabolic engineering are discussed. Finally, the article explores the limitations and future prospects of microbial biotin production. This comprehensive review not only discusses strategies for biotin enhancement but also provides in-depth insights into systematic metabolic engineering approaches aimed at boosting biotin production.

show abstract

Detection and Characterization of a Thermophilic Biotin Biosynthetic Enzyme, 7-Keto-8-aminopelargonic Acid Synthase, from Various Thermophiles

Cited by 4 publications

References 34 publications

Analysis of In Vivo Transcriptome of Intracellular Bacterial Pathogen Salmonella enterica serovar Typhmurium Isolated from Mouse Spleen

Analysis of In Vivo Transcriptome of Intracellular Bacterial Pathogen Salmonella enterica serovar Typhmurium Isolated from Mouse Spleen

Screening and Identification of Key Genes for the Survival and Multiplication of Salmonella typhimurium in the Host

Advances and prospects in microbial production of biotin

Contact Info

Product

Resources

About