1995
DOI: 10.1128/mcb.15.3.1769
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Detection and Characterization of a 3′ Untranslated Region Ribonucleoprotein Complex Associated with Human α-Globin mRNA Stability

Abstract: The highly stable nature of globin mRNA is of central importance to erythroid cell differentiation. We have previously identified cytidine-rich (C-rich) segments in the human ␣-globin mRNA 3 untranslated region (␣-3UTR) which are critical in the maintenance of mRNA stability in transfected erythroid cells. In the present studies, we have detected trans-acting factors which interact with these cis elements to mediate this stabilizing function. A sequence-specific ribonucleoprotein (RNP) complex is assembled aft… Show more

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Cited by 192 publications
(227 citation statements)
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“…The eluate from the poly(C)-agarose restored fully formation of the complex with HIPBS RNA or the TH transcript in the binding reactions. This finding differs from previously reported data showing that the poly(C)-eluted proteins did not restore formation of the complex associated with ␣ 2 -globin stability element and was thus considered necessary but not sufficient for the complex formation (10). Further, formation of the globin-associated complex seems to require additional protein factors such as AUF1 (hnRNPD) protein (19).…”
contrasting
confidence: 56%
See 1 more Smart Citation
“…The eluate from the poly(C)-agarose restored fully formation of the complex with HIPBS RNA or the TH transcript in the binding reactions. This finding differs from previously reported data showing that the poly(C)-eluted proteins did not restore formation of the complex associated with ␣ 2 -globin stability element and was thus considered necessary but not sufficient for the complex formation (10). Further, formation of the globin-associated complex seems to require additional protein factors such as AUF1 (hnRNPD) protein (19).…”
contrasting
confidence: 56%
“…A similar procedure was used previously to purify the protein factor binding to the cytidine-rich elements in ␣ 2 -globin mRNA (9,10). As expected, we purified the 40-kDa poly(C)-binding protein that corresponds to PCBP (12), also known as ␣CP (9, 10) or hnRNPE (17,18), as identified by the specific antibodies.…”
mentioning
confidence: 99%
“…There are many similarities between the regulation of ␣1(I) collagen mRNA in activated HSCs and the regulation of ␣-globin and lipoxygenase mRNAs in erythroid cells and the tyrosine hydroxylase mRNA in response to hypoxia (17,(47)(48)(49). These mRNAs form a class of molecules post-transcriptionally regulated via 3Ј-UTR interactions with protein(s).…”
Section: Discussionmentioning
confidence: 99%
“…Both the 3Ј-CR and 3Ј-UTR sequence in the 68-nt fragment are necessary for assembly of a unique C-binding RNP complex at this site (1). Interestingly, the stability of the ␣-globin mRNA is also regulated by a determinant on the proximal edge of the 3Ј-UTR that is dependent upon sequence in the adjacent 3Ј-CR (25,26).…”
Section: Discussionmentioning
confidence: 99%