Detection and characterisation of human metapneumovirus from children with acute respiratory symptoms in north-west England, UK

Hopkins, Mark; Redmond, Catherine; Shaw, Janet M.; Hart, Ian; Smyth, Rosalind L; Semple, Malcolm G

doi:10.1016/j.jcv.2008.03.011

Cited by 16 publications

(14 citation statements)

References 33 publications

(42 reference statements)

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“…Co-circulation of two or more sub-clusters is not unusual as similar findings have been reported from countries all over the world, e.g. United States [9], Australia [12], [44], United Kingdom [45], Croatia [36], Japan [46], China [47], South Korea [48], Brazil [49] and France [50]. It was suggested, that outbreaks of HMPV subgroups and sub-clusters appear to be a local phenomenon unlike to influenza, which spreads across the continents with two or three viruses [30].…”

Section: Discussionsupporting

confidence: 83%

Human Metapneumovirus: Insights from a Ten-Year Molecular and Epidemiological Analysis in Germany

et al. 2014

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Human metapneumovirus (HMPV) is a cause of respiratory tract illness at all ages. In this study the epidemiological and molecular diversity among patients of different ages was investigated. Between 2000–2001 and 2009–2010, HMPV was detected in 3% (138/4,549) of samples from outpatients with influenza-like illness with a new, sensitive real-time RT-PCR assay. Several hundred (797) clinical specimens from hospitalized children below the age of 4 years with acute respiratory illness were investigated and HMPV was detected in 11.9% of them. Investigation of outpatients revealed that HMPV infections occurred in individuals of all ages but were most prevalent in children (0–4 years) and the elderly (>60 years). The most present clinical features of HMPV infections were cough, bronchitis, fever/shivers and pneumonia. About two thirds of HMPV-positive samples were detected in February and March throughout the study period. Molecular characterization of HMPV revealed a complex cyclic pattern of group dominance where HMPV subgroup A and B viruses predominated in general for three consecutive seasons. German HMPV represented all genetic lineages including A1, A2, B1, B2, sub-clusters A2a and A2b. For Germany, not only time-dependent circulation of lineages and sub-clusters was observed but also co-circulation of two or three predominant lineages. Two newly emerging amino acid substitutions (positions 223 and 280) of lineage B2 were detected in seven German HMPV sequences. Our study gives new insights into the molecular epidemiology of HMPV in in- and outpatients over a time period of 10 years for the first time. It is one of only few long-term surveillance studies in Europe, and allows comparative molecular analyses of HMPV circulating worldwide.

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Section: Discussionsupporting

confidence: 83%

Human Metapneumovirus: Insights from a Ten-Year Molecular and Epidemiological Analysis in Germany

et al. 2014

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“…Although identification of HMPV in clinical samples is usually not a major diagnostic challenge, genetic heterogeneity among HMPV strains can lead to false negative RT-PCR assays, leading some investigators to seek further improvement in these assays 4,5. Our findings imply that the DOP-PCR based universal virus assay is useful for finding viruses in patient samples where other methods have failed to identify a pathogen.…”

Section: Discussionmentioning

confidence: 88%

Use of a universal virus detection assay to identify human metapneumovirus in a hematopoietic stem cell transplant recipient with pneumonia of unknown origin

Uhlenhaut

Cohen

Fedorko

et al. 2009

Journal of Clinical Virology

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“…Three hundred fifty-two partial or full N sequences were aligned with published real-time RT-PCR assays targeting the N gene 14-18,20,21 . Many of these were partial and non-overlapping, so that the number of viral sequences compared against any single primer/probe ranged from 45 to 85 (not shown).…”

Section: Resultsmentioning

confidence: 99%

Real-time reverse transcriptase PCR assay for improved detection of human metapneumovirus

Ali

Johnson

et al. 2012

Journal of Clinical Virology

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Background Human metapneumovirus (HMPV) is a paramyxovirus with multiple genetic lineages that is a leading cause of acute respiratory disease. Several RT-PCR assays have been described based on limited available sequence data. Objectives To develop a broadly reactive real-time RT-PCR assay for HMPV that allows for a rapid, sensitive, and specific detection in a clinical or research setting. Study Design Three published assays for HMPV were modified based on analysis of multiple HMPV sequences obtained from GenBank. Original and modified assays were tested against prototype HMPV strains from each genetic sublineage, multiple isolates of HMPV from different years, a collection of clinical specimens, and commercial validation panels. Results A number of potential sequence mismatches with diverse HMPV strains were identified. Modifications were made to oligonucleotides to improve annealing efficiency. Primers and probes based on newer sequence data offered enhanced detection of all subgroups, especially for low titer specimens. The new primers and probe detected multiple clinical isolates of HMPV collected over a twenty-year period. The modified assay improved detection of HMPV in a panel of clinical specimens, and correctly identified HMPV samples in two commercial validation sets. Conclusions We report a modified real-time RT-PCR assay for HMPV that detects all genetic lineages with high sensitivity.

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Detection and characterisation of human metapneumovirus from children with acute respiratory symptoms in north-west England, UK

Cited by 16 publications

References 33 publications

Human Metapneumovirus: Insights from a Ten-Year Molecular and Epidemiological Analysis in Germany

Human Metapneumovirus: Insights from a Ten-Year Molecular and Epidemiological Analysis in Germany

Use of a universal virus detection assay to identify human metapneumovirus in a hematopoietic stem cell transplant recipient with pneumonia of unknown origin

Real-time reverse transcriptase PCR assay for improved detection of human metapneumovirus

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