Detection and beyond: challenges and advances in aptamer-based biosensors

Yoo, Hyebin; Jo, Hyesung; Oh, Seung Soo

doi:10.1039/d0ma00639d

Cited by 174 publications

(93 citation statements)

References 189 publications

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“…This is exemplified in Figure 15A with the assembly of a DNA tweezer triggered by ligand-aptamer complexes into "open" and "closed" configurations [204]. The tweezer in the closed state I is composed of two arms (15) and (16) interlocked by two bridging strands, (17) and (18), that hybridize with counter complementary domains associated with the arms (15) and (16). The arms (15) and (16) include sequence-specific domains corresponding to the adenosine monophosphate aptamer, AMP aptamer.…”

Section: Dna Motor Systems Driven By Aptamer-ligand Complexesmentioning

confidence: 99%

“…The tweezer in the closed state I is composed of two arms (15) and (16) interlocked by two bridging strands, (17) and (18), that hybridize with counter complementary domains associated with the arms (15) and (16). The arms (15) and (16) include sequence-specific domains corresponding to the adenosine monophosphate aptamer, AMP aptamer. In the presence of AMP, the formation of the AMP ligand/AMP aptamer complexes releases the strand (18), resulting in the formation of the open tweezer configuration, state II.…”

Section: Dna Motor Systems Driven By Aptamer-ligand Complexesmentioning

confidence: 99%

“…The sequence-specific binding properties of aptamers have been widely applied to develop sensing platforms [ 12 , 13 , 14 , 15 ]. By the integration of aptamers with nanomaterials, hybrid optical sensing platforms that combine the specific recognition properties of aptamers with the unique optical or physical properties of nanomaterials were assembled [ 87 , 88 , 89 ].…”

Section: Hybrid Aptamer Nanostructures For Sensing Applicationsmentioning

confidence: 99%

“…The eliciting of sequence-specific nucleic acids (aptamers) recognizing low-molecular-weight ligands, macromolecules and even cells by the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) procedure [ 1 , 2 , 3 , 4 ] introduced revolutionary means to apply nucleic acids as functional materials for bioanalytical [ 5 , 6 ], catalytic [ 7 , 8 , 9 ] and biomedical [ 9 , 10 , 11 , 12 ] applications. The selective formation of aptamer-ligand complexes enabled the application of aptamers as versatile recognition elements for the development of sensor devices and sensing platforms, aptasensors [ 12 , 13 , 14 , 15 ]. By the immobilization of aptamers on appropriate transducers, numerous electrochemical [ 16 , 17 , 18 , 19 ], photoelectrochemical [ 20 , 21 , 22 , 23 ], surface plasmon resonance (SPR) [ 24 , 25 , 26 ], microgravimetric quartz-crystal-microbalance (QCM) [ 27 , 28 , 29 , 30 ], magnetic [ 31 , 32 , 33 ] and acoustic [ 34 , 35 , 36 ] sensors were demonstrated.…”

Section: Introductionmentioning

confidence: 99%

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Aptamer-Functionalized Hybrid Nanostructures for Sensing, Drug Delivery, Catalysis and Mechanical Applications

Vázquez‐González

Willner

2021

IJMS

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Sequence-specific nucleic acids exhibiting selective recognition properties towards low-molecular-weight substrates and macromolecules (aptamers) find growing interest as functional biopolymers for analysis, medical applications such as imaging, drug delivery and even therapeutic agents, nanotechnology, material science and more. The present perspective article introduces a glossary of examples for diverse applications of aptamers mainly originated from our laboratory. These include the introduction of aptamer-functionalized nanomaterials such as graphene oxide, Ag nanoclusters and semiconductor quantum dots as functional hybrid nanomaterials for optical sensing of target analytes. The use of aptamer-functionalized DNA tetrahedra nanostructures for multiplex analysis and aptamer-loaded metal-organic framework nanoparticles acting as sense-and-treat are introduced. Aptamer-functionalized nano and microcarriers are presented as stimuli-responsive hybrid drug carriers for controlled and targeted drug release, including aptamer-functionalized SiO2 nanoparticles, carbon dots, metal-organic frameworks and microcapsules. A further application of aptamers involves the conjugation of aptamers to catalytic units as a means to mimic enzyme functions “nucleoapzymes”. In addition, the formation and dissociation of aptamer-ligand complexes are applied to develop mechanical molecular devices and to switch nanostructures such as origami scaffolds. Finally, the article discusses future challenges in applying aptamers in material science, nanotechnology and catalysis.

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Section: Dna Motor Systems Driven By Aptamer-ligand Complexesmentioning

confidence: 99%

Section: Dna Motor Systems Driven By Aptamer-ligand Complexesmentioning

confidence: 99%

Section: Hybrid Aptamer Nanostructures For Sensing Applicationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Aptamer-Functionalized Hybrid Nanostructures for Sensing, Drug Delivery, Catalysis and Mechanical Applications

Vázquez‐González

Willner

2021

IJMS

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“…POC tests and clinical treatments require the measurement of diagnostically relevant protein biomarkers. Aptamers recognize proteins more quickly than small molecules or ions [ 75 ]. Identifying the presence or absence of pathogens or species-specific proteins is vital in clinical settings.…”

Section: Future Directionsmentioning

confidence: 99%

BipD of Burkholderia pseudomallei: Structure, Functions, and Detection Methods

et al. 2021

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Melioidosis is a severe disease caused by Burkholderia pseudomallei (B. pseudomallei), a Gram-negative environmental bacterium. It is endemic in Southeast Asia and Northern Australia, but it is underreported in many other countries. The principal routes of entry for B. pseudomallei are skin penetration, inhalation, and ingestion. It mainly affects immunocompromised populations, especially patients with type 2 diabetes mellitus. The laboratory diagnosis of melioidosis is challenging due to its non-specific clinical manifestations, which mimic other severe infections. The culture method is considered an imperfect gold standard for the diagnosis of melioidosis due to its low sensitivity. Antibody detection has low sensitivity and specificity due to the high seropositivity among healthy people in endemic regions. Antigen detection using various proteins has been tested for the rapid determination of B. pseudomallei; however, it presents certain limitations in terms of its sensitivity and specificity. Therefore, this review aims to frame the present knowledge of a potential target known as the Burkholderia invasion protein D (BipD), including future directions for its detection using an aptamer-based sensor (aptasensor).

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Biointerface Engineering with Nucleic Acid Materials for Biosensing Applications

Shi

Chen

Wang

et al. 2022

Adv Funct Materials

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Molecular recognition at the biointerface plays a critical role in sensing molecular interactions (e.g., DNA hybridization) and extracellular changes, which can directly affect the detection performance of biosensors (e.g., sensitivity, specificity, and response dynamics). However, conventional sensing biointerfaces show low molecular recognition efficiency due to limited target accessibility. Engineering sensing biointerfaces to regulate the orientation, spacing, and density of surface‐confined molecular probes offer an effective approach to improve molecular recognition at interfaces. Over the last decades, biointerface engineering with nucleic acid materials has advanced the fundamental understanding of DNA hybridization kinetics and facilitated the design of improved biosensing platforms for monitoring cellular activities and diagnosing relevant diseases. This review summarizes the recent progress in nucleic acid‐based biointerface engineering. The development of nucleic acid materials that can be applied to specific diagnostic applications is briefly introduced. Then the roles of nucleic acids in tailoring the properties of nanosurfaces, cell surfaces, and macroscopic surfaces are discussed and their biosensing applications are comprehensively highlighted. Finally, future challenges and perspectives of emerging technologies and applications in the field are presented.

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Detection and beyond: challenges and advances in aptamer-based biosensors

Abstract: Beyond traditional needs of biosensors such as high sensitivity and selectivity for analyte detection, newly emerging requirements including a real-time detection ability and in-field applicability have been gradually emphasized to...

Cited by 174 publications

References 189 publications

Aptamer-Functionalized Hybrid Nanostructures for Sensing, Drug Delivery, Catalysis and Mechanical Applications

Aptamer-Functionalized Hybrid Nanostructures for Sensing, Drug Delivery, Catalysis and Mechanical Applications

BipD of Burkholderia pseudomallei: Structure, Functions, and Detection Methods

Biointerface Engineering with Nucleic Acid Materials for Biosensing Applications

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