Detection and Behavior Study of Some Genetically Engineered Microorganisms Released in Soil Using Plate Counting, Gene Transfer Assay and Specific PCR Methods
Abstract:The objective of this study was monitoring and behavior study of genetically engineered microorganisms (GEMs) that might be accidentally or deliberately released into the environments. Three methods (plate count, gene transfer and specific PCR) were used with four genetically engineered E. coli strains (A:pJan25, B: pJan25, pGWB533 and pGWB404) harboring antibiotics resistance genes with additional gfp and gus genes as a molecular markers. The GEMs were added to soil microcosms at 10 7 cells/g soil and incubat… Show more
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